##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BfrI BglII BlnI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspLU11I BsrBI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI Hin1I KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AclI 456 AACGTT AA^CGTT
AcsI 724 GAATTT [GA]^AATT[CT]
AocI 1873 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1780 AGCGCT AGC^GCT
ApoI 724 GAATTT [GA]^AATT[CT]
AspI 82 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 396 CCCGAG C^[CT]CG[GA]G
BanI 625 GGCACC G^G[CT][GA]CC
BclI 593 TGATCA T^GATCA
BcnI 853 CCCGG CC^[GC]GG
BglI 1209 GCCAGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 606 TACGTG [CT]AC^GT[GA]
BsaBI 968 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1975 GAATGC GAATGC(1/-1)
BsoBI 396 CCCGAG C^[CT]CG[GA]G
BspHI 2085 TCATGA T^CATGA
BspMI 1698 ACCTGC ACCTGC(4/8)
BsrDI 138 CATTGC GCAATG(2/0)
BsrFI 622 ACCGGC [GA]^CCGG[CT]
Bsu36I 1873 CCTCAGG CC^T[ACGT]AGG
CfoI 1781 GCGC GCG^C
Cfr10I 622 ACCGGC [GA]^CCGG[CT]
Eco47III 1780 AGCGCT AGC^GCT
Eco81I 1873 CCTCAGG CC^T[ACGT]AGG
FbaI 593 TGATCA T^GATCA
HaeII 1780 AGCGCT [GA]GCGC^[CT]
HhaI 1781 GCGC GCG^C
HinP1I 1781 GCGC G^CGC
HindIII 690 AAGCTT A^AGCTT
HpaI 2122 GTTAAC GTT^AAC
MamI 968 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 853 CCCGG CC^[GC]GG
NcoI 2003 CCATGG C^CATGG
PflFI 82 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 456 AACGTT AA^CGTT
RcaI 2085 TCATGA T^CATGA
TaqI 779 TCGA T^CGA
Tth111I 82 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 779 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
BalI 179 TGGCCA TGG^CCA
1679 TGGCCA
BlpI 1253 GCTCAGC GC^T[ACGT]AGC
1990 GCTGAGC
Bpu1102I 1253 GCTCAGC GC^T[ACGT]AGC
1990 GCTGAGC
BsaI 1905 GAGACC GGTCTC(1/5)
2007 GGTCTC
Bsp1407I 874 TGTACA T^GTACA
2323 TGTACA
BsrGI 874 TGTACA T^GTACA
2323 TGTACA
CelII 1253 GCTCAGC GC^T[ACGT]AGC
1990 GCTGAGC
DsaI 286 CCGTGG C^C[GA][CT]GG
2003 CCATGG
EaeI 179 TGGCCA [CT]^GGCC[GA]
1679 TGGCCA
EcoNI 1430 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1947 CCTTCACCAGG
EcoT14I 823 CCAAGG C^C[AT][AT]GG
2003 CCATGG
HapII 623 CCGG C^CGG
854 CCGG
HgaI 82 GACGC GACGC(5/10)
540 GACGC
HincII 1843 GTTGAC GT[CT]^[GA]AC
2122 GTTAAC
HindII 1843 GTTGAC GT[CT]^[GA]AC
2122 GTTAAC
HpaII 623 CCGG C^CGG
854 CCGG
MluNI 179 TGGCCA TGG^CCA
1679 TGGCCA
MscI 179 TGGCCA TGG^CCA
1679 TGGCCA
MslI 1151 CACTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2038 CACTTCTGTG
MspI 623 CCGG C^CGG
854 CCGG
PflMI 1037 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1508 CCAGGAAATGG
PleI 451 GACTC GAGTC(4/5)
2017 GAGTC
PpuMI 1830 GGGACCT [GA]G^G[AT]CC[CT]
1915 GGGACCC
SacI 1251 GAGCTC GAGCT^C
1449 GAGCTC
SspBI 874 TGTACA T^GTACA
2323 TGTACA
StuI 400 AGGCCT AGG^CCT
1427 AGGCCT
StyI 823 CCAAGG C^C[AT][AT]GG
2003 CCATGG
Tsp45I 1753 GTGAC ^GT[GC]AC
2101 GTCAC
Van91I 1037 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1508 CCAGGAAATGG
XbaI 1316 TCTAGA T^CTAGA
2028 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
Asp700I AspHI AvaII BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI TfiI
Tru9I TseI Tsp509I TspRI XcmI
XhoII XmnI