##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AclI AflIII AgeI AocI Aor51HI
AscI AseI AsnI Asp700I AviII
BalI BamHI BbrPI BclI BsaAI
BsaBI BspDI BspHI BspLU11I BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
FbaI FspI MamI MfeI MluI
MluNI MscI MunI NcoI NdeI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SbfI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
TfiI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 3227 GACGTC GACGT^C
Acc65I 4845 GGTACC G^GTACC
AccI 2484 GTCGAC GT^[AC][GT]AC
AccIII 4015 TCCGGA T^CCGGA
AcsI 3743 GAATTT [GA]^AATT[CT]
AflII 254 CTTAAG C^TTAAG
AhdI 957 GACCGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 42 GTGCAC G^TGCAC
ApaLI 42 GTGCAC G^TGCAC
ApoI 3743 GAATTT [GA]^AATT[CT]
Asp718I 4845 GGTACC G^GTACC
AspEI 957 GACCGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4942 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 344 GTATCC GTATCC(6/5)
BfrI 254 CTTAAG C^TTAAG
BglI 3360 GCCGGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 4015 TCCGGA [AT]^CCGG[AT]
BseAI 4015 TCCGGA T^CCGGA
BsmI 4778 GCATTC GAATGC(1/-1)
Bsp1407I 4181 TGTACA T^GTACA
BspEI 4015 TCCGGA T^CCGGA
BsrDI 4615 GCAATG GCAATG(2/0)
BsrGI 4181 TGTACA T^GTACA
Eam1105I 957 GACCGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 2950 GATATC GAT^ATC
EcoT22I 3079 ATGCAT ATGCA^T
FseI 1017 GGCCGGCC GGCCGG^CC
HpaI 3872 GTTAAC GTT^AAC
KpnI 4845 GGTACC GGTAC^C
KspI 1395 CCGCGG CCGC^GG
MroI 4015 TCCGGA T^CCGGA
NsiI 3079 ATGCAT ATGCA^T
PflFI 4942 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2920 CCATGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4199 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 1395 CCGCGG CCGC^GG
SalI 2484 GTCGAC G^TCGAC
SexAI 4726 ACCAGGT A^CC[AT]GGT
SphI 2275 GCATGC GCATG^C
SspBI 4181 TGTACA T^GTACA
Tth111I 4942 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2920 CCATGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AvrII 2931 CCTAGG C^CTAGG
3941 CCTAGG
BbsI 114 GAAGAC GAAGAC(2/6)
954 GAAGAC
BglII 4890 AGATCT A^GATCT
5026 AGATCT
BlnI 2931 CCTAGG C^CTAGG
3941 CCTAGG
BpuAI 114 GAAGAC GAAGAC(2/6)
954 GAAGAC
BsiEI 482 CGACCG CG[GA][CT]^CG
5573 CGGTCG
BsmBI 1212 GAGACG CGTCTC(1/5)
2100 GAGACG
BssSI 2397 CACGAG CACGAG(-5/-1)
3289 CACGAG
DraI 3814 TTTAAA TTT^AAA
5032 TTTAAA
EaeI 661 TGGCCG [CT]^GGCC[GA]
2371 TGGCCG
StuI 631 AGGCCT AGG^CCT
4277 AGGCCT
XbaI 2096 TCTAGA T^CTAGA
4898 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbeI BbvCI
BbvI BcgI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiWI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DrdI DsaI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI SspI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII