##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI Aor51HI ApaI AscI Asp718I
AspI AvrII BamHI BbeI BbrPI
BcgI BfrI BlnI BsaWI BseAI
BsiWI BspEI BssSI Bst1107I BstXI
BstZ17I CpoI DrdI EagI EclXI
Eco47III Eco52I FseI HpaI KasI
KpnI KspI MluI MroI NarI
NheI NotI NruI PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SnaBI
Sse8387I StuI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 4315 GACGTC GACGT^C
AcyI 4315 GACGTC G[GA]^CG[CT]C
Alw44I 2366 GTGCAC G^TGCAC
AocI 3252 CCTCAGG CC^T[ACGT]AGG
ApaLI 2366 GTGCAC G^TGCAC
Asp700I 1275 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 5012 TGCGCA TGC^GCA
BclI 4219 TGATCA T^GATCA
BglI 4909 GCCTCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2334 TACGTG [CT]AC^GT[GA]
BsaBI 1046 GATAGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 4315 GACGTC G[GA]^CG[CT]C
BsiEI 666 CGACCG CG[GA][CT]^CG
BsmBI 4303 GAGACG CGTCTC(1/5)
BspDI 4012 ATCGAT AT^CGAT
BsrBI 2244 GAGCGG CCGCTC(-3/-3)
BssHII 121 GCGCGC G^CGCGC
BstEII 4581 GGTCACC G^GT[ACGT]ACC
BstPI 4581 GGTCACC G^GT[ACGT]ACC
Bsu36I 3252 CCTCAGG CC^T[ACGT]AGG
ClaI 4012 ATCGAT AT^CGAT
Eco81I 3252 CCTCAGG CC^T[ACGT]AGG
EcoNI 5084 CCTGATATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4581 GGTCACC G^GT[ACGT]ACC
EcoRI 4602 GAATTC G^AATTC
EcoRV 1050 GATATC GAT^ATC
EcoT22I 2356 ATGCAT ATGCA^T
FbaI 4219 TGATCA T^GATCA
FspI 5012 TGCGCA TGC^GCA
Hin1I 4315 GACGTC G[GA]^CG[CT]C
HincII 833 GTCAAC GT[CT]^[GA]AC
HindII 833 GTCAAC GT[CT]^[GA]AC
HindIII 1478 AAGCTT A^AGCTT
MamI 1046 GATAGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3910 CAATTG C^AATTG
MunI 3910 CAATTG C^AATTG
NaeI 4455 GCCGGC GCC^GGC
NcoI 2888 CCATGG C^CATGG
NgoMIV 4455 GCCGGC G^CCGGC
NsiI 2356 ATGCAT ATGCA^T
PshAI 4225 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2484 GAGCTC GAGCT^C
SmaI 26 CCCGGG CCC^GGG
XbaI 3018 TCTAGA T^CTAGA
XmaCI 26 CCCGGG C^CCGGG
XmaI 26 CCCGGG C^CCGGG
XmnI 1275 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AhdI 594 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
754 GACTGTTTGTC
AspEI 594 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
754 GACTGTTTGTC
BalI 1455 TGGCCA TGG^CCA
3158 TGGCCA
BanI 3090 GGCACC G^G[CT][GA]CC
4656 GGTGCC
BbvCI 4430 CCTCAGC CCTCAGC(-5/-2)
2266 GCTGAGG
BciVI 3029 GGATAC GTATCC(6/5)
1175 GTATCC
BsmI 722 GAATGC GAATGC(1/-1)
4036 GCATTC
BspHI 2228 TCATGA T^CATGA
4216 TCATGA
BsrDI 3924 CATTGC GCAATG(2/0)
3657 GCAATG
BsrFI 2508 GCCGGT [GA]^CCGG[CT]
4455 GCCGGC
BstBI 3821 TTCGAA TT^CGAA
5520 TTCGAA
Cfr10I 2508 GCCGGT [GA]^CCGG[CT]
4455 GCCGGC
DraIII 1635 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
5551 CACATGGTG
DsaI 47 CCACGG C^C[GA][CT]GG
2888 CCATGG
EaeI 1455 TGGCCA [CT]^GGCC[GA]
3158 TGGCCA
Eam1105I 594 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
754 GACTGTTTGTC
HaeII 107 GGCGCT [GA]GCGC^[CT]
4205 GGCGCT
MluNI 1455 TGGCCA TGG^CCA
3158 TGGCCA
MscI 1455 TGGCCA TGG^CCA
3158 TGGCCA
NdeI 1593 CATATG CA^TATG
1789 CATATG
NspV 3821 TTCGAA TT^CGAA
5520 TTCGAA
PacI 1353 TTAATTAA TTAAT^TAA
5452 TTAATTAA
PflMI 3506 CCAAAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5550 CCACATGGTGG
RcaI 2228 TCATGA T^CATGA
4216 TCATGA
SfuI 3821 TTCGAA TT^CGAA
5520 TTCGAA
SpeI 4195 ACTAGT A^CTAGT
5133 ACTAGT
Van91I 3506 CCAAAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5550 CCACATGGTGG
XcmI 364 CCAGGAGGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5543 CCAGCAACCACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI AspHI AvaI AvaII BanII
BbsI BbvI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspLU11I BspMI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PaeR7I PleI PpuMI PshBI
PstI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SphI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoI XhoII