##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspI AviII
AvrII BanI BbeI BbrPI BglI
BlnI BsaAI BseAI BsiEI BsiWI
BspEI BspLU11I BsrBI BsrFI BssHII
BstEII BstPI Cfr10I CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI FseI FspI
HaeII HincII HindII HpaI KasI
KpnI KspI MluI MroI NaeI
NarI NgoMIV NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I StuI SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AflII 2557 CTTAAG C^TTAAG
AflIII 1411 ACGTGT A^C[GA][CT]GT
AhdI 580 GACCCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 580 GACCCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 67 CCCGAG C^[CT]CG[GA]G
BalI 3173 TGGCCA TGG^CCA
BamHI 2239 GGATCC G^GATCC
BbvCI 4594 CCTCAGC CCTCAGC(-5/-2)
BfrI 2557 CTTAAG C^TTAAG
BglII 1392 AGATCT A^GATCT
BlpI 5006 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 5006 GCTTAGC GC^T[ACGT]AGC
BsaWI 1545 TCCGGT [AT]^CCGG[AT]
BsgI 2927 CTGCAC GTGCAG(16/14)
BsmBI 480 GAGACG CGTCTC(1/5)
BsmI 3620 GCATTC GAATGC(1/-1)
BsoBI 67 CCCGAG C^[CT]CG[GA]G
Bsp1407I 3978 TGTACA T^GTACA
BspDI 3820 ATCGAT AT^CGAT
BspMI 5047 ACCTGC ACCTGC(4/8)
BsrGI 3978 TGTACA T^GTACA
BssSI 376 CACGAG CACGAG(-5/-1)
Bst1107I 3804 GTATAC GTA^TAC
BstXI 2019 CCAAGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3804 GTATAC GTA^TAC
CelII 5006 GCTTAGC GC^T[ACGT]AGC
ClaI 3820 ATCGAT AT^CGAT
DraII 4648 GGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 580 GACCCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 4648 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 1056 GATATC GAT^ATC
MfeI 1019 CAATTG C^AATTG
MluNI 3173 TGGCCA TGG^CCA
MscI 3173 TGGCCA TGG^CCA
MunI 1019 CAATTG C^AATTG
NcoI 4151 CCATGG C^CATGG
PpuMI 4648 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 2788 GACTTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 72 GAGCTC GAGCT^C
SapI 682 GAAGAGC GCTCTTC(1/4)
SspBI 3978 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 3 CGCG CG^CG
56 CGCG
AcyI 51 GGCGTC G[GA]^CG[CT]C
1516 GACGCC
AocI 1510 CCTGAGG CC^T[ACGT]AGG
3427 CCTAAGG
BanII 72 GAGCTC G[GA]GC[CT]^C
2033 GGGCTC
BcgI 2392 CGACACCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4743 GCAAACTGATCG
BciVI 3417 GGATAC GTATCC(6/5)
2152 GTATCC
BclI 605 TGATCA T^GATCA
2589 TGATCA
BcnI 25 CCGGG CC^[GC]GG
2947 CCGGG
BsaBI 1479 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3961 GATTCTCATC
BsaHI 51 GGCGTC G[GA]^CG[CT]C
1516 GACGCC
BspHI 1139 TCATGA T^CATGA
3225 TCATGA
BsrDI 3809 CATTGC GCAATG(2/0)
3399 GCAATG
BstBI 1725 TTCGAA TT^CGAA
3791 TTCGAA
BstUI 3 CGCG CG^CG
56 CGCG
Bsu36I 1510 CCTGAGG CC^T[ACGT]AGG
3427 CCTAAGG
DsaI 4151 CCATGG C^C[GA][CT]GG
4641 CCACGG
EaeI 22 TGGCCG [CT]^GGCC[GA]
3173 TGGCCA
Eco81I 1510 CCTGAGG CC^T[ACGT]AGG
3427 CCTAAGG
EcoT22I 224 ATGCAT ATGCA^T
303 ATGCAT
FbaI 605 TGATCA T^GATCA
2589 TGATCA
Hin1I 51 GGCGTC G[GA]^CG[CT]C
1516 GACGCC
MamI 1479 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3961 GATTCTCATC
MvnI 3 CGCG CG^CG
56 CGCG
NciI 25 CCGGG CC^[GC]GG
2947 CCGGG
NheI 276 GCTAGC G^CTAGC
3073 GCTAGC
NsiI 224 ATGCAT ATGCA^T
303 ATGCAT
NspV 1725 TTCGAA TT^CGAA
3791 TTCGAA
PflMI 2866 CCAAACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3630 CCAGAAGCTGG
RcaI 1139 TCATGA T^CATGA
3225 TCATGA
ScaI 310 AGTACT AGT^ACT
448 AGTACT
SfuI 1725 TTCGAA TT^CGAA
3791 TTCGAA
SmlI 1164 CTCAAG C^T[CT][GA]AG
2557 CTTAAG
SphI 331 GCATGC GCATG^C
534 GCATGC
Van91I 2866 CCAAACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3630 CCAGAAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XcmI XhoII
XmnI