################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspI AviII AvrII BanI BbeI BbrPI BglI BlnI BsaAI BseAI BsiEI BsiWI BspEI BspLU11I BsrBI BsrFI BssHII BstEII BstPI Cfr10I CpoI DraIII DrdI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI FseI FspI HaeII HincII HindII HpaI KasI KpnI KspI MluI MroI NaeI NarI NgoMIV NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I StuI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AflII 2557 CTTAAG C^TTAAG AflIII 1411 ACGTGT A^C[GA][CT]GT AhdI 580 GACCCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 580 GACCCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 67 CCCGAG C^[CT]CG[GA]G BalI 3173 TGGCCA TGG^CCA BamHI 2239 GGATCC G^GATCC BbvCI 4594 CCTCAGC CCTCAGC(-5/-2) BfrI 2557 CTTAAG C^TTAAG BglII 1392 AGATCT A^GATCT BlpI 5006 GCTTAGC GC^T[ACGT]AGC Bpu1102I 5006 GCTTAGC GC^T[ACGT]AGC BsaWI 1545 TCCGGT [AT]^CCGG[AT] BsgI 2927 CTGCAC GTGCAG(16/14) BsmBI 480 GAGACG CGTCTC(1/5) BsmI 3620 GCATTC GAATGC(1/-1) BsoBI 67 CCCGAG C^[CT]CG[GA]G Bsp1407I 3978 TGTACA T^GTACA BspDI 3820 ATCGAT AT^CGAT BspMI 5047 ACCTGC ACCTGC(4/8) BsrGI 3978 TGTACA T^GTACA BssSI 376 CACGAG CACGAG(-5/-1) Bst1107I 3804 GTATAC GTA^TAC BstXI 2019 CCAAGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 3804 GTATAC GTA^TAC CelII 5006 GCTTAGC GC^T[ACGT]AGC ClaI 3820 ATCGAT AT^CGAT DraII 4648 GGGACCT [GA]G^G[ACGT]CC[CT] Eam1105I 580 GACCCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 4648 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRV 1056 GATATC GAT^ATC MfeI 1019 CAATTG C^AATTG MluNI 3173 TGGCCA TGG^CCA MscI 3173 TGGCCA TGG^CCA MunI 1019 CAATTG C^AATTG NcoI 4151 CCATGG C^CATGG PpuMI 4648 GGGACCT [GA]G^G[AT]CC[CT] PshAI 2788 GACTTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 72 GAGCTC GAGCT^C SapI 682 GAAGAGC GCTCTTC(1/4) SspBI 3978 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccII 3 CGCG CG^CG 56 CGCG AcyI 51 GGCGTC G[GA]^CG[CT]C 1516 GACGCC AocI 1510 CCTGAGG CC^T[ACGT]AGG 3427 CCTAAGG BanII 72 GAGCTC G[GA]GC[CT]^C 2033 GGGCTC BcgI 2392 CGACACCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4743 GCAAACTGATCG BciVI 3417 GGATAC GTATCC(6/5) 2152 GTATCC BclI 605 TGATCA T^GATCA 2589 TGATCA BcnI 25 CCGGG CC^[GC]GG 2947 CCGGG BsaBI 1479 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3961 GATTCTCATC BsaHI 51 GGCGTC G[GA]^CG[CT]C 1516 GACGCC BspHI 1139 TCATGA T^CATGA 3225 TCATGA BsrDI 3809 CATTGC GCAATG(2/0) 3399 GCAATG BstBI 1725 TTCGAA TT^CGAA 3791 TTCGAA BstUI 3 CGCG CG^CG 56 CGCG Bsu36I 1510 CCTGAGG CC^T[ACGT]AGG 3427 CCTAAGG DsaI 4151 CCATGG C^C[GA][CT]GG 4641 CCACGG EaeI 22 TGGCCG [CT]^GGCC[GA] 3173 TGGCCA Eco81I 1510 CCTGAGG CC^T[ACGT]AGG 3427 CCTAAGG EcoT22I 224 ATGCAT ATGCA^T 303 ATGCAT FbaI 605 TGATCA T^GATCA 2589 TGATCA Hin1I 51 GGCGTC G[GA]^CG[CT]C 1516 GACGCC MamI 1479 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3961 GATTCTCATC MvnI 3 CGCG CG^CG 56 CGCG NciI 25 CCGGG CC^[GC]GG 2947 CCGGG NheI 276 GCTAGC G^CTAGC 3073 GCTAGC NsiI 224 ATGCAT ATGCA^T 303 ATGCAT NspV 1725 TTCGAA TT^CGAA 3791 TTCGAA PflMI 2866 CCAAACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3630 CCAGAAGCTGG RcaI 1139 TCATGA T^CATGA 3225 TCATGA ScaI 310 AGTACT AGT^ACT 448 AGTACT SfuI 1725 TTCGAA TT^CGAA 3791 TTCGAA SmlI 1164 CTCAAG C^T[CT][GA]AG 2557 CTTAAG SphI 331 GCATGC GCATG^C 534 GCATGC Van91I 2866 CCAAACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3630 CCAGAAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BbsI BbvI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XcmI XhoII XmnI