################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## AccII AccIII AgeI AhdI Aor51HI AscI AseI AsnI AspEI AviII BbeI BbrPI BcgI BsaWI BseAI BsiWI BsmBI BspDI BspEI BsrBI BssHII BstBI BstUI ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FseI FspI KasI KspI MluI MroI MvnI NarI NheI NotI NruI NsiI NspV PaeR7I PinAI PmaCI PmeI PmlI PshBI RsrII SacI SacII SfiI SfoI SfuI SgrAI SnaBI SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 576 GACGTC GACGT^C Acc65I 2974 GGTACC G^GTACC AclI 2389 AACGTT AA^CGTT Alw44I 2988 GTGCAC G^TGCAC AocI 530 CCTGAGG CC^T[ACGT]AGG ApaLI 2988 GTGCAC G^TGCAC Asp718I 2974 GGTACC G^GTACC BamHI 5755 GGATCC G^GATCC BbvCI 3102 GCTGAGG CCTCAGC(-5/-2) BglI 2625 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 3599 GCTCAGC GC^T[ACGT]AGC Bpu1102I 3599 GCTCAGC GC^T[ACGT]AGC BsaBI 4756 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 4402 CGATCG CG[GA][CT]^CG BsmI 3607 GCATTC GAATGC(1/-1) BspHI 1660 TCATGA T^CATGA BssSI 3486 CACGAG CACGAG(-5/-1) Bst1107I 2549 GTATAC GTA^TAC BstZ17I 2549 GTATAC GTA^TAC Bsu36I 530 CCTGAGG CC^T[ACGT]AGG CelII 3599 GCTCAGC GC^T[ACGT]AGC DraI 1711 TTTAAA TTT^AAA Eco81I 530 CCTGAGG CC^T[ACGT]AGG FauI 4836 CCCGC CCCGC(4/6) HaeII 4616 AGCGCC [GA]GCGC^[CT] HindIII 3314 AAGCTT A^AGCTT HpaI 1265 GTTAAC GTT^AAC KpnI 2974 GGTACC GGTAC^C MamI 4756 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1453 CAATTG C^AATTG MunI 1453 CAATTG C^AATTG NaeI 3911 GCCGGC GCC^GGC NdeI 5429 CATATG CA^TATG NgoMIV 3911 GCCGGC G^CCGGC PacI 1786 TTAATTAA TTAAT^TAA PshAI 3699 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 2389 AACGTT AA^CGTT PvuI 4402 CGATCG CGAT^CG RcaI 1660 TCATGA T^CATGA SalI 6026 GTCGAC G^TCGAC SapI 6067 GCTCTTC GCTCTTC(1/4) ScaI 1749 AGTACT AGT^ACT SmaI 399 CCCGGG CCC^GGG SpeI 3417 ACTAGT A^CTAGT XbaI 4024 TCTAGA T^CTAGA XmaCI 399 CCCGGG C^CCGGG XmaI 399 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcyI 576 GACGTC G[GA]^CG[CT]C 2622 GACGCC AflII 182 CTTAAG C^TTAAG 6244 CTTAAG Asp700I 4877 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 6132 GAAATCCTTC AspI 5605 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 5744 GACAATGTC BciVI 2286 GTATCC GTATCC(6/5) 3551 GTATCC BclI 1438 TGATCA T^GATCA 3341 TGATCA BfrI 182 CTTAAG C^TTAAG 6244 CTTAAG BsaAI 3836 TACGTG [CT]AC^GT[GA] 5340 TACGTG BsaHI 576 GACGTC G[GA]^CG[CT]C 2622 GACGCC BsrDI 4272 CATTGC GCAATG(2/0) 785 GCAATG BsrFI 623 ACCGGC [GA]^CCGG[CT] 3911 GCCGGC BstAPI 5106 GCATACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5359 GCAGTTAGTGC CfoI 4318 GCGC GCG^C 4617 GCGC Cfr10I 623 ACCGGC [GA]^CCGG[CT] 3911 GCCGGC DraIII 464 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG 1132 CACTGAGTG EcoNI 1489 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2878 CCTTGGATAGG FbaI 1438 TGATCA T^GATCA 3341 TGATCA HgaI 2622 GACGC GACGC(5/10) 52 GCGTC HhaI 4318 GCGC GCG^C 4617 GCGC Hin1I 576 GACGTC G[GA]^CG[CT]C 2622 GACGCC HinP1I 4318 GCGC G^CGC 4617 GCGC PflFI 5605 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 5744 GACAATGTC PflMI 1647 CCAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4061 CCAAATTTTGG SbfI 1492 CCTGCAGG CCTGCA^GG 1595 CCTGCAGG SphI 1056 GCATGC GCATG^C 4071 GCATGC Sse8387I 1492 CCTGCAGG CCTGCA^GG 1595 CCTGCAGG TaqI 585 TCGA T^CGA 6027 TCGA Tth111I 5605 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 5744 GACAATGTC TthHB8I 585 TCGA T^CGA 6027 TCGA Van91I 1647 CCAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4061 CCAAATTTTGG XmnI 4877 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 6132 GAAATCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII AvrII BalI BanI BanII BbsI BbvI BcnI BfaI BglII BlnI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspLU11I BspMI BsrGI BsrI BssKI BstEII BstF5I BstNI BstPI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI EcoO109I EcoO65I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI SspBI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII