##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccII AccIII AgeI AhdI Aor51HI
AscI AseI AsnI AspEI AviII
BbeI BbrPI BcgI BsaWI BseAI
BsiWI BsmBI BspDI BspEI BsrBI
BssHII BstBI BstUI ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI FspI
KasI KspI MluI MroI MvnI
NarI NheI NotI NruI NsiI
NspV PaeR7I PinAI PmaCI PmeI
PmlI PshBI RsrII SacI SacII
SfiI SfoI SfuI SgrAI SnaBI
SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 576 GACGTC GACGT^C
Acc65I 2974 GGTACC G^GTACC
AclI 2389 AACGTT AA^CGTT
Alw44I 2988 GTGCAC G^TGCAC
AocI 530 CCTGAGG CC^T[ACGT]AGG
ApaLI 2988 GTGCAC G^TGCAC
Asp718I 2974 GGTACC G^GTACC
BamHI 5755 GGATCC G^GATCC
BbvCI 3102 GCTGAGG CCTCAGC(-5/-2)
BglI 2625 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3599 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3599 GCTCAGC GC^T[ACGT]AGC
BsaBI 4756 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 4402 CGATCG CG[GA][CT]^CG
BsmI 3607 GCATTC GAATGC(1/-1)
BspHI 1660 TCATGA T^CATGA
BssSI 3486 CACGAG CACGAG(-5/-1)
Bst1107I 2549 GTATAC GTA^TAC
BstZ17I 2549 GTATAC GTA^TAC
Bsu36I 530 CCTGAGG CC^T[ACGT]AGG
CelII 3599 GCTCAGC GC^T[ACGT]AGC
DraI 1711 TTTAAA TTT^AAA
Eco81I 530 CCTGAGG CC^T[ACGT]AGG
FauI 4836 CCCGC CCCGC(4/6)
HaeII 4616 AGCGCC [GA]GCGC^[CT]
HindIII 3314 AAGCTT A^AGCTT
HpaI 1265 GTTAAC GTT^AAC
KpnI 2974 GGTACC GGTAC^C
MamI 4756 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1453 CAATTG C^AATTG
MunI 1453 CAATTG C^AATTG
NaeI 3911 GCCGGC GCC^GGC
NdeI 5429 CATATG CA^TATG
NgoMIV 3911 GCCGGC G^CCGGC
PacI 1786 TTAATTAA TTAAT^TAA
PshAI 3699 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2389 AACGTT AA^CGTT
PvuI 4402 CGATCG CGAT^CG
RcaI 1660 TCATGA T^CATGA
SalI 6026 GTCGAC G^TCGAC
SapI 6067 GCTCTTC GCTCTTC(1/4)
ScaI 1749 AGTACT AGT^ACT
SmaI 399 CCCGGG CCC^GGG
SpeI 3417 ACTAGT A^CTAGT
XbaI 4024 TCTAGA T^CTAGA
XmaCI 399 CCCGGG C^CCGGG
XmaI 399 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 576 GACGTC G[GA]^CG[CT]C
2622 GACGCC
AflII 182 CTTAAG C^TTAAG
6244 CTTAAG
Asp700I 4877 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6132 GAAATCCTTC
AspI 5605 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5744 GACAATGTC
BciVI 2286 GTATCC GTATCC(6/5)
3551 GTATCC
BclI 1438 TGATCA T^GATCA
3341 TGATCA
BfrI 182 CTTAAG C^TTAAG
6244 CTTAAG
BsaAI 3836 TACGTG [CT]AC^GT[GA]
5340 TACGTG
BsaHI 576 GACGTC G[GA]^CG[CT]C
2622 GACGCC
BsrDI 4272 CATTGC GCAATG(2/0)
785 GCAATG
BsrFI 623 ACCGGC [GA]^CCGG[CT]
3911 GCCGGC
BstAPI 5106 GCATACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5359 GCAGTTAGTGC
CfoI 4318 GCGC GCG^C
4617 GCGC
Cfr10I 623 ACCGGC [GA]^CCGG[CT]
3911 GCCGGC
DraIII 464 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
1132 CACTGAGTG
EcoNI 1489 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2878 CCTTGGATAGG
FbaI 1438 TGATCA T^GATCA
3341 TGATCA
HgaI 2622 GACGC GACGC(5/10)
52 GCGTC
HhaI 4318 GCGC GCG^C
4617 GCGC
Hin1I 576 GACGTC G[GA]^CG[CT]C
2622 GACGCC
HinP1I 4318 GCGC G^CGC
4617 GCGC
PflFI 5605 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5744 GACAATGTC
PflMI 1647 CCAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4061 CCAAATTTTGG
SbfI 1492 CCTGCAGG CCTGCA^GG
1595 CCTGCAGG
SphI 1056 GCATGC GCATG^C
4071 GCATGC
Sse8387I 1492 CCTGCAGG CCTGCA^GG
1595 CCTGCAGG
TaqI 585 TCGA T^CGA
6027 TCGA
Tth111I 5605 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5744 GACAATGTC
TthHB8I 585 TCGA T^CGA
6027 TCGA
Van91I 1647 CCAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4061 CCAAATTTTGG
XmnI 4877 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6132 GAAATCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII AvrII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglII BlnI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspLU11I BspMI
BsrGI BsrI BssKI BstEII BstF5I
BstNI BstPI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI EcoO109I EcoO65I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI SspBI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII