##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII Acc65I AccIII AgeI AscI
AseI AsnI Asp718I AvrII BbeI
BcgI BciVI BlnI BseAI BsiWI
BsmBI BspEI BspLU11I BsrBI BssHII
CpoI EagI EclXI Eco52I FseI
KasI KpnI KspI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NruI PacI PinAI PmeI
PshBI PvuI RsrII SacII SalI
SapI SbfI SfiI SfoI SgrAI
SnaBI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AflII 5564 CTTAAG C^TTAAG
Alw44I 1260 GTGCAC G^TGCAC
Aor51HI 2012 AGCGCT AGC^GCT
ApaI 4637 GGGCCC GGGCC^C
ApaLI 1260 GTGCAC G^TGCAC
BamHI 4465 GGATCC G^GATCC
BfrI 5564 CTTAAG C^TTAAG
BglII 2691 AGATCT A^GATCT
BlpI 1822 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1822 GCTCAGC GC^T[ACGT]AGC
BsaBI 4852 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 744 ACCGGA [AT]^CCGG[AT]
BsiEI 1863 CGACCG CG[GA][CT]^CG
Bsp1407I 684 TGTACA T^GTACA
BspDI 947 ATCGAT AT^CGAT
BsrGI 684 TGTACA T^GTACA
Bst1107I 5176 GTATAC GTA^TAC
BstEII 2311 GGTCACC G^GT[ACGT]ACC
BstPI 2311 GGTCACC G^GT[ACGT]ACC
BstZ17I 5176 GTATAC GTA^TAC
CelII 1822 GCTCAGC GC^T[ACGT]AGC
ClaI 947 ATCGAT AT^CGAT
DraI 5641 TTTAAA TTT^AAA
Eco47III 2012 AGCGCT AGC^GCT
EcoO65I 2311 GGTCACC G^GT[ACGT]ACC
EcoRI 3827 GAATTC G^AATTC
EcoT22I 4906 ATGCAT ATGCA^T
HpaI 5687 GTTAAC GTT^AAC
MamI 4852 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 3746 GCCGGC GCC^GGC
NgoMIV 3746 GCCGGC G^CCGGC
NsiI 4906 ATGCAT ATGCA^T
PaeR7I 839 CTCGAG C^TCGAG
PflMI 979 CCAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI 3450 AGTACT AGT^ACT
SpeI 2534 ACTAGT A^CTAGT
SphI 3299 GCATGC GCATG^C
SspBI 684 TGTACA T^GTACA
Van91I 979 CCAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 955 TCTAGA T^CTAGA
XhoI 839 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AclI 5555 AACGTT AA^CGTT
6503 AACGTT
AcyI 988 GGCGTC G[GA]^CG[CT]C
3317 GACGCC
AhdI 368 GACATTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3259 GACAGCAGGTC
AspEI 368 GACATTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3259 GACAGCAGGTC
AspI 232 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1952 GACACAGTC
AviII 2397 TGCGCA TGC^GCA
4078 TGCGCA
BclI 316 TGATCA T^GATCA
3150 TGATCA
BsaHI 988 GGCGTC G[GA]^CG[CT]C
3317 GACGCC
BsmI 670 GAATGC GAATGC(1/-1)
6377 GCATTC
BsrDI 4114 GCAATG GCAATG(2/0)
4158 GCAATG
BsrFI 3746 GCCGGC [GA]^CCGG[CT]
5382 GCCGGT
BstBI 441 TTCGAA TT^CGAA
4004 TTCGAA
Cfr10I 3746 GCCGGC [GA]^CCGG[CT]
5382 GCCGGT
DrdI 2303 GACCAGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4937 GACATCCGAGTC
Eam1105I 368 GACATTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3259 GACAGCAGGTC
FbaI 316 TGATCA T^GATCA
3150 TGATCA
FspI 2397 TGCGCA TGC^GCA
4078 TGCGCA
HaeII 497 AGCGCC [GA]GCGC^[CT]
2012 AGCGCT
Hin1I 988 GGCGTC G[GA]^CG[CT]C
3317 GACGCC
NspV 441 TTCGAA TT^CGAA
4004 TTCGAA
PflFI 232 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1952 GACACAGTC
PshAI 2819 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4749 GACCTCGGTC
Psp1406I 5555 AACGTT AA^CGTT
6503 AACGTT
PstI 226 CTGCAG CTGCA^G
5475 CTGCAG
SfuI 441 TTCGAA TT^CGAA
4004 TTCGAA
SmaI 3822 CCCGGG CCC^GGG
4641 CCCGGG
StuI 831 AGGCCT AGG^CCT
1480 AGGCCT
Tth111I 232 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1952 GACACAGTC
XmaCI 3822 CCCGGG C^CCGGG
4641 CCCGGG
XmaI 3822 CCCGGG C^CCGGG
4641 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbrPI BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoRV EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PmaCI PmlI PpuMI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI