################################## # NO CUT ENZYMES ( 53 enzymes) # ################################## AatII Acc65I AccIII AgeI AscI AseI AsnI Asp718I AvrII BbeI BcgI BciVI BlnI BseAI BsiWI BsmBI BspEI BspLU11I BsrBI BssHII CpoI EagI EclXI Eco52I FseI KasI KpnI KspI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI PacI PinAI PmeI PshBI PvuI RsrII SacII SalI SapI SbfI SfiI SfoI SgrAI SnaBI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AflII 5564 CTTAAG C^TTAAG Alw44I 1260 GTGCAC G^TGCAC Aor51HI 2012 AGCGCT AGC^GCT ApaI 4637 GGGCCC GGGCC^C ApaLI 1260 GTGCAC G^TGCAC BamHI 4465 GGATCC G^GATCC BfrI 5564 CTTAAG C^TTAAG BglII 2691 AGATCT A^GATCT BlpI 1822 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1822 GCTCAGC GC^T[ACGT]AGC BsaBI 4852 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 744 ACCGGA [AT]^CCGG[AT] BsiEI 1863 CGACCG CG[GA][CT]^CG Bsp1407I 684 TGTACA T^GTACA BspDI 947 ATCGAT AT^CGAT BsrGI 684 TGTACA T^GTACA Bst1107I 5176 GTATAC GTA^TAC BstEII 2311 GGTCACC G^GT[ACGT]ACC BstPI 2311 GGTCACC G^GT[ACGT]ACC BstZ17I 5176 GTATAC GTA^TAC CelII 1822 GCTCAGC GC^T[ACGT]AGC ClaI 947 ATCGAT AT^CGAT DraI 5641 TTTAAA TTT^AAA Eco47III 2012 AGCGCT AGC^GCT EcoO65I 2311 GGTCACC G^GT[ACGT]ACC EcoRI 3827 GAATTC G^AATTC EcoT22I 4906 ATGCAT ATGCA^T HpaI 5687 GTTAAC GTT^AAC MamI 4852 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 3746 GCCGGC GCC^GGC NgoMIV 3746 GCCGGC G^CCGGC NsiI 4906 ATGCAT ATGCA^T PaeR7I 839 CTCGAG C^TCGAG PflMI 979 CCAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ScaI 3450 AGTACT AGT^ACT SpeI 2534 ACTAGT A^CTAGT SphI 3299 GCATGC GCATG^C SspBI 684 TGTACA T^GTACA Van91I 979 CCAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 955 TCTAGA T^CTAGA XhoI 839 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AclI 5555 AACGTT AA^CGTT 6503 AACGTT AcyI 988 GGCGTC G[GA]^CG[CT]C 3317 GACGCC AhdI 368 GACATTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3259 GACAGCAGGTC AspEI 368 GACATTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3259 GACAGCAGGTC AspI 232 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1952 GACACAGTC AviII 2397 TGCGCA TGC^GCA 4078 TGCGCA BclI 316 TGATCA T^GATCA 3150 TGATCA BsaHI 988 GGCGTC G[GA]^CG[CT]C 3317 GACGCC BsmI 670 GAATGC GAATGC(1/-1) 6377 GCATTC BsrDI 4114 GCAATG GCAATG(2/0) 4158 GCAATG BsrFI 3746 GCCGGC [GA]^CCGG[CT] 5382 GCCGGT BstBI 441 TTCGAA TT^CGAA 4004 TTCGAA Cfr10I 3746 GCCGGC [GA]^CCGG[CT] 5382 GCCGGT DrdI 2303 GACCAGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4937 GACATCCGAGTC Eam1105I 368 GACATTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3259 GACAGCAGGTC FbaI 316 TGATCA T^GATCA 3150 TGATCA FspI 2397 TGCGCA TGC^GCA 4078 TGCGCA HaeII 497 AGCGCC [GA]GCGC^[CT] 2012 AGCGCT Hin1I 988 GGCGTC G[GA]^CG[CT]C 3317 GACGCC NspV 441 TTCGAA TT^CGAA 4004 TTCGAA PflFI 232 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1952 GACACAGTC PshAI 2819 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4749 GACCTCGGTC Psp1406I 5555 AACGTT AA^CGTT 6503 AACGTT PstI 226 CTGCAG CTGCA^G 5475 CTGCAG SfuI 441 TTCGAA TT^CGAA 4004 TTCGAA SmaI 3822 CCCGGG CCC^GGG 4641 CCCGGG StuI 831 AGGCCT AGG^CCT 1480 AGGCCT Tth111I 232 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1952 GACACAGTC XmaCI 3822 CCCGGG C^CCGGG 4641 CCCGGG XmaI 3822 CCCGGG C^CCGGG 4641 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 134 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbrPI BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoRV EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PmaCI PmlI PpuMI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI