################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## Acc65I AccIII AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BciVI BclI BlnI BsaWI BseAI BsiEI BsiWI BspDI BspEI BsrFI BssHII Bst1107I BstBI BstZ17I Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRV FbaI FseI FspI HpaI KpnI KspI MfeI MluI MroI MunI NaeI NgoMIV NheI NotI NruI NspV PacI PinAI PmeI PshAI PshBI PvuI RsrII SacII SalI SbfI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 1622 GACGTC GACGT^C AccII 3105 CGCG CG^CG AclI 191 AACGTT AA^CGTT AflII 5294 CTTAAG C^TTAAG Alw44I 4317 GTGCAC G^TGCAC ApaLI 4317 GTGCAC G^TGCAC Asp700I 5448 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbeI 1239 GGCGCC GGCGC^C BbrPI 4696 CACGTG CAC^GTG BcnI 3307 CCGGG CC^[GC]GG BfrI 5294 CTTAAG C^TTAAG BglII 2556 AGATCT A^GATCT BsaAI 4696 CACGTG [CT]AC^GT[GA] BsmI 796 GCATTC GAATGC(1/-1) Bsp1407I 3462 TGTACA T^GTACA BspLU11I 69 ACATGT A^CATGT BsrBI 3080 GAGCGG CCGCTC(-3/-3) BsrDI 4427 GCAATG GCAATG(2/0) BsrGI 3462 TGTACA T^GTACA BssSI 862 CTCGTG CACGAG(-5/-1) BstAPI 3696 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 3105 CGCG CG^CG CfoI 1240 GCGC GCG^C HaeII 1239 GGCGCC [GA]GCGC^[CT] HhaI 1240 GCGC GCG^C HinP1I 1240 GCGC G^CGC HincII 1847 GTCAAC GT[CT]^[GA]AC HindII 1847 GTCAAC GT[CT]^[GA]AC KasI 1239 GGCGCC G^GCGCC MvnI 3105 CGCG CG^CG NarI 1239 GGCGCC GG^CGCC NciI 3307 CCGGG CC^[GC]GG PmaCI 4696 CACGTG CAC^GTG PmlI 4696 CACGTG CAC^GTG Psp1406I 191 AACGTT AA^CGTT SacI 3284 GAGCTC GAGCT^C SapI 3661 GAAGAGC GCTCTTC(1/4) SexAI 135 ACCAGGT A^CC[AT]GGT SfiI 1444 GGCCCAACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1239 GGCGCC GGC^GCC SspBI 3462 TGTACA T^GTACA SspI 5650 AATATT AAT^ATT XbaI 4474 TCTAGA T^CTAGA XmnI 5448 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 65 GTAGAC GT^[AC][GT]AC 2591 GTAGAC AflIII 69 ACATGT A^C[GA][CT]GT 4697 ACGTGT ApaI 1537 GGGCCC GGGCC^C 3309 GGGCCC AvaI 1939 CTCGAG C^[CT]CG[GA]G 3345 CTCGAG BbvCI 2297 CCTCAGC CCTCAGC(-5/-2) 3208 GCTGAGG BcgI 1941 CGAGTGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4112 CGAGATTTCTGC BglI 1445 GCCCAACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4379 GCCAAGCTGGC BsaBI 373 GATTGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3382 GATGAGAATC BsmBI 1620 GAGACG CGTCTC(1/5) 2942 GAGACG BsoBI 1939 CTCGAG C^[CT]CG[GA]G 3345 CTCGAG BspHI 708 TCATGA T^CATGA 1776 TCATGA BspMI 83 ACCTGC ACCTGC(4/8) 3198 ACCTGC BstEII 2105 GGTGACC G^GT[ACGT]ACC 4045 GGTGACC BstPI 2105 GGTGACC G^GT[ACGT]ACC 4045 GGTGACC BstXI 785 CCAGAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2929 CCATGGTTTTGG DraI 4862 TTTAAA TTT^AAA 5608 TTTAAA EcoO65I 2105 GGTGACC G^GT[ACGT]ACC 4045 GGTGACC EcoT22I 2645 ATGCAT ATGCA^T 4185 ATGCAT FauI 3607 GCGGG CCCGC(4/6) 4042 GCGGG HapII 512 CCGG C^CGG 3307 CCGG HgaI 1040 GACGC GACGC(5/10) 3186 GACGC HindIII 600 AAGCTT A^AGCTT 2072 AAGCTT HpaII 512 CCGG C^CGG 3307 CCGG MamI 373 GATTGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3382 GATGAGAATC MspI 512 CCGG C^CGG 3307 CCGG NcoI 2616 CCATGG C^CATGG 2929 CCATGG NdeI 2642 CATATG CA^TATG 5674 CATATG NsiI 2645 ATGCAT ATGCA^T 4185 ATGCAT PaeR7I 1939 CTCGAG C^TCGAG 3345 CTCGAG RcaI 708 TCATGA T^CATGA 1776 TCATGA SphI 397 GCATGC GCATG^C 1320 GCATGC XhoI 1939 CTCGAG C^TCGAG 3345 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApoI AspHI AspI AvaII BalI BanI BanII BbsI BbvI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CelII Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII Hin1I HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII