##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AccIII AgeI AhdI Aor51HI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BamHI BciVI BclI
BlnI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BsrFI BssHII Bst1107I
BstBI BstZ17I Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV FbaI FseI FspI
HpaI KpnI KspI MfeI MluI
MroI MunI NaeI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmeI PshAI PshBI PvuI RsrII
SacII SalI SbfI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 1622 GACGTC GACGT^C
AccII 3105 CGCG CG^CG
AclI 191 AACGTT AA^CGTT
AflII 5294 CTTAAG C^TTAAG
Alw44I 4317 GTGCAC G^TGCAC
ApaLI 4317 GTGCAC G^TGCAC
Asp700I 5448 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 1239 GGCGCC GGCGC^C
BbrPI 4696 CACGTG CAC^GTG
BcnI 3307 CCGGG CC^[GC]GG
BfrI 5294 CTTAAG C^TTAAG
BglII 2556 AGATCT A^GATCT
BsaAI 4696 CACGTG [CT]AC^GT[GA]
BsmI 796 GCATTC GAATGC(1/-1)
Bsp1407I 3462 TGTACA T^GTACA
BspLU11I 69 ACATGT A^CATGT
BsrBI 3080 GAGCGG CCGCTC(-3/-3)
BsrDI 4427 GCAATG GCAATG(2/0)
BsrGI 3462 TGTACA T^GTACA
BssSI 862 CTCGTG CACGAG(-5/-1)
BstAPI 3696 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 3105 CGCG CG^CG
CfoI 1240 GCGC GCG^C
HaeII 1239 GGCGCC [GA]GCGC^[CT]
HhaI 1240 GCGC GCG^C
HinP1I 1240 GCGC G^CGC
HincII 1847 GTCAAC GT[CT]^[GA]AC
HindII 1847 GTCAAC GT[CT]^[GA]AC
KasI 1239 GGCGCC G^GCGCC
MvnI 3105 CGCG CG^CG
NarI 1239 GGCGCC GG^CGCC
NciI 3307 CCGGG CC^[GC]GG
PmaCI 4696 CACGTG CAC^GTG
PmlI 4696 CACGTG CAC^GTG
Psp1406I 191 AACGTT AA^CGTT
SacI 3284 GAGCTC GAGCT^C
SapI 3661 GAAGAGC GCTCTTC(1/4)
SexAI 135 ACCAGGT A^CC[AT]GGT
SfiI 1444 GGCCCAACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1239 GGCGCC GGC^GCC
SspBI 3462 TGTACA T^GTACA
SspI 5650 AATATT AAT^ATT
XbaI 4474 TCTAGA T^CTAGA
XmnI 5448 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 65 GTAGAC GT^[AC][GT]AC
2591 GTAGAC
AflIII 69 ACATGT A^C[GA][CT]GT
4697 ACGTGT
ApaI 1537 GGGCCC GGGCC^C
3309 GGGCCC
AvaI 1939 CTCGAG C^[CT]CG[GA]G
3345 CTCGAG
BbvCI 2297 CCTCAGC CCTCAGC(-5/-2)
3208 GCTGAGG
BcgI 1941 CGAGTGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4112 CGAGATTTCTGC
BglI 1445 GCCCAACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4379 GCCAAGCTGGC
BsaBI 373 GATTGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3382 GATGAGAATC
BsmBI 1620 GAGACG CGTCTC(1/5)
2942 GAGACG
BsoBI 1939 CTCGAG C^[CT]CG[GA]G
3345 CTCGAG
BspHI 708 TCATGA T^CATGA
1776 TCATGA
BspMI 83 ACCTGC ACCTGC(4/8)
3198 ACCTGC
BstEII 2105 GGTGACC G^GT[ACGT]ACC
4045 GGTGACC
BstPI 2105 GGTGACC G^GT[ACGT]ACC
4045 GGTGACC
BstXI 785 CCAGAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2929 CCATGGTTTTGG
DraI 4862 TTTAAA TTT^AAA
5608 TTTAAA
EcoO65I 2105 GGTGACC G^GT[ACGT]ACC
4045 GGTGACC
EcoT22I 2645 ATGCAT ATGCA^T
4185 ATGCAT
FauI 3607 GCGGG CCCGC(4/6)
4042 GCGGG
HapII 512 CCGG C^CGG
3307 CCGG
HgaI 1040 GACGC GACGC(5/10)
3186 GACGC
HindIII 600 AAGCTT A^AGCTT
2072 AAGCTT
HpaII 512 CCGG C^CGG
3307 CCGG
MamI 373 GATTGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3382 GATGAGAATC
MspI 512 CCGG C^CGG
3307 CCGG
NcoI 2616 CCATGG C^CATGG
2929 CCATGG
NdeI 2642 CATATG CA^TATG
5674 CATATG
NsiI 2645 ATGCAT ATGCA^T
4185 ATGCAT
PaeR7I 1939 CTCGAG C^TCGAG
3345 CTCGAG
RcaI 708 TCATGA T^CATGA
1776 TCATGA
SphI 397 GCATGC GCATG^C
1320 GCATGC
XhoI 1939 CTCGAG C^TCGAG
3345 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AspI AvaII BalI BanI BanII
BbsI BbvI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CelII Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII Hin1I
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII