################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AatII AclI AgeI AocI AscI AviII BbeI BbrPI BcgI BlpI Bpu1102I BsiWI Bsp1407I BspDI BspHI BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I CelII ClaI CpoI DrdI Eco81I EcoT22I FseI FspI HindIII HpaI KasI KspI MfeI MluI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I SspBI SspI SwaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### Acc65I 3339 GGTACC G^GTACC AccIII 3250 TCCGGA T^CCGGA AcsI 4941 GAATTC [GA]^AATT[CT] AflII 3192 CTTAAG C^TTAAG AhdI 634 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 4941 GAATTC [GA]^AATT[CT] AseI 2367 ATTAAT AT^TAAT AsnI 2367 ATTAAT AT^TAAT Asp718I 3339 GGTACC G^GTACC AspEI 634 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BciVI 1907 GTATCC GTATCC(6/5) BclI 3652 TGATCA T^GATCA BfrI 3192 CTTAAG C^TTAAG BsaAI 416 CACGTA [CT]AC^GT[GA] BsaBI 3650 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1307 GAGACC GGTCTC(1/5) BsaWI 3250 TCCGGA [AT]^CCGG[AT] BseAI 3250 TCCGGA T^CCGGA BsmBI 2834 CGTCTC CGTCTC(1/5) BspEI 3250 TCCGGA T^CCGGA BspLU11I 2298 ACATGT A^CATGT BssSI 3382 CTCGTG CACGAG(-5/-1) DraI 5114 TTTAAA TTT^AAA DraIII 1131 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 634 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 4941 GAATTC G^AATTC EcoRV 1551 GATATC GAT^ATC FbaI 3652 TGATCA T^GATCA KpnI 3339 GGTACC GGTAC^C MamI 3650 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 3250 TCCGGA T^CCGGA NcoI 3343 CCATGG C^CATGG PshBI 2367 ATTAAT AT^TAAT PvuII 1152 CAGCTG CAG^CTG SacI 1236 GAGCTC GAGCT^C SpeI 4212 ACTAGT A^CTAGT XbaI 1760 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 834 CGCG CG^CG 2136 CGCG Asp700I 1734 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2189 GAACCACTTC AvrII 2705 CCTAGG C^CTAGG 4553 CCTAGG BglII 28 AGATCT A^GATCT 2350 AGATCT BlnI 2705 CCTAGG C^CTAGG 4553 CCTAGG BsiEI 1692 CGGCCG CG[GA][CT]^CG 3935 CGGCCG BsrBI 3161 CCGCTC CCGCTC(-3/-3) 536 GAGCGG BstAPI 239 GCAGAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3627 GCAGATGCTGC BstEII 318 GGTGACC G^GT[ACGT]ACC 2873 GGTGACC BstPI 318 GGTGACC G^GT[ACGT]ACC 2873 GGTGACC BstUI 834 CGCG CG^CG 2136 CGCG BstXI 1468 CCAGTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4461 CCAGGGCCCTGG EagI 1692 CGGCCG C^GGCCG 3935 CGGCCG EclXI 1692 CGGCCG C^GGCCG 3935 CGGCCG Eco52I 1692 CGGCCG C^GGCCG 3935 CGGCCG EcoO65I 318 GGTGACC G^GT[ACGT]ACC 2873 GGTGACC MvnI 834 CGCG CG^CG 2136 CGCG NspI 1223 GCATGT [GA]CATG^[CT] 2298 ACATGT PaeR7I 1327 CTCGAG C^TCGAG 4167 CTCGAG PshAI 159 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2919 GACTCTAGTC ScaI 2695 AGTACT AGT^ACT 5079 AGTACT SexAI 2857 ACCTGGT A^CC[AT]GGT 4120 ACCAGGT SmaI 934 CCCGGG CCC^GGG 3838 CCCGGG XhoI 1327 CTCGAG C^TCGAG 4167 CTCGAG XmaCI 934 CCCGGG C^CCGGG 3838 CCCGGG XmaI 934 CCCGGG C^CCGGG 3838 CCCGGG XmnI 1734 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2189 GAACCACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 129 enzymes) # ######################################### AccI AciI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI AspHI AspI AvaI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco47III EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PflFI PflMI PleI PpuMI PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII