##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AclI AgeI AocI AscI
AviII BbeI BbrPI BcgI BlpI
Bpu1102I BsiWI Bsp1407I BspDI BspHI
BsrGI BssHII Bst1107I BstBI BstZ17I
Bsu36I CelII ClaI CpoI DrdI
Eco81I EcoT22I FseI FspI HindIII
HpaI KasI KspI MfeI MluI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SfiI SfoI SfuI SgrAI SnaBI
SphI Sse8387I SspBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 3339 GGTACC G^GTACC
AccIII 3250 TCCGGA T^CCGGA
AcsI 4941 GAATTC [GA]^AATT[CT]
AflII 3192 CTTAAG C^TTAAG
AhdI 634 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 4941 GAATTC [GA]^AATT[CT]
AseI 2367 ATTAAT AT^TAAT
AsnI 2367 ATTAAT AT^TAAT
Asp718I 3339 GGTACC G^GTACC
AspEI 634 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 1907 GTATCC GTATCC(6/5)
BclI 3652 TGATCA T^GATCA
BfrI 3192 CTTAAG C^TTAAG
BsaAI 416 CACGTA [CT]AC^GT[GA]
BsaBI 3650 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1307 GAGACC GGTCTC(1/5)
BsaWI 3250 TCCGGA [AT]^CCGG[AT]
BseAI 3250 TCCGGA T^CCGGA
BsmBI 2834 CGTCTC CGTCTC(1/5)
BspEI 3250 TCCGGA T^CCGGA
BspLU11I 2298 ACATGT A^CATGT
BssSI 3382 CTCGTG CACGAG(-5/-1)
DraI 5114 TTTAAA TTT^AAA
DraIII 1131 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 634 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4941 GAATTC G^AATTC
EcoRV 1551 GATATC GAT^ATC
FbaI 3652 TGATCA T^GATCA
KpnI 3339 GGTACC GGTAC^C
MamI 3650 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3250 TCCGGA T^CCGGA
NcoI 3343 CCATGG C^CATGG
PshBI 2367 ATTAAT AT^TAAT
PvuII 1152 CAGCTG CAG^CTG
SacI 1236 GAGCTC GAGCT^C
SpeI 4212 ACTAGT A^CTAGT
XbaI 1760 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 834 CGCG CG^CG
2136 CGCG
Asp700I 1734 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2189 GAACCACTTC
AvrII 2705 CCTAGG C^CTAGG
4553 CCTAGG
BglII 28 AGATCT A^GATCT
2350 AGATCT
BlnI 2705 CCTAGG C^CTAGG
4553 CCTAGG
BsiEI 1692 CGGCCG CG[GA][CT]^CG
3935 CGGCCG
BsrBI 3161 CCGCTC CCGCTC(-3/-3)
536 GAGCGG
BstAPI 239 GCAGAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3627 GCAGATGCTGC
BstEII 318 GGTGACC G^GT[ACGT]ACC
2873 GGTGACC
BstPI 318 GGTGACC G^GT[ACGT]ACC
2873 GGTGACC
BstUI 834 CGCG CG^CG
2136 CGCG
BstXI 1468 CCAGTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4461 CCAGGGCCCTGG
EagI 1692 CGGCCG C^GGCCG
3935 CGGCCG
EclXI 1692 CGGCCG C^GGCCG
3935 CGGCCG
Eco52I 1692 CGGCCG C^GGCCG
3935 CGGCCG
EcoO65I 318 GGTGACC G^GT[ACGT]ACC
2873 GGTGACC
MvnI 834 CGCG CG^CG
2136 CGCG
NspI 1223 GCATGT [GA]CATG^[CT]
2298 ACATGT
PaeR7I 1327 CTCGAG C^TCGAG
4167 CTCGAG
PshAI 159 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2919 GACTCTAGTC
ScaI 2695 AGTACT AGT^ACT
5079 AGTACT
SexAI 2857 ACCTGGT A^CC[AT]GGT
4120 ACCAGGT
SmaI 934 CCCGGG CCC^GGG
3838 CCCGGG
XhoI 1327 CTCGAG C^TCGAG
4167 CTCGAG
XmaCI 934 CCCGGG C^CCGGG
3838 CCCGGG
XmaI 934 CCCGGG C^CCGGG
3838 CCCGGG
XmnI 1734 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2189 GAACCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI Aor51HI
ApaI ApaLI AspHI AspI AvaI
AvaII BalI BamHI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco47III
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PflFI PflMI PleI
PpuMI PstI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII