################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## AatII AccII AclI AcyI AgeI Aor51HI ApaI AscI AvaI AvrII BbeI BcgI BciVI BclI BglII BlnI BlpI Bpu1102I BsaHI BsiEI BsiWI BsoBI Bsp1407I BspDI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I CelII Cfr10I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoNI FbaI FseI HaeII HgaI Hin1I KasI KspI MfeI MluI MunI MvnI NaeI NarI NdeI NgoMIV NotI NruI NspV PaeR7I PinAI PmeI PpuMI PshAI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 5821 GGTACC G^GTACC AccIII 2381 TCCGGA T^CCGGA AhdI 5082 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2695 CCTAAGG CC^T[ACGT]AGG Asp700I 311 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 5821 GGTACC G^GTACC AspEI 5082 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 4979 TGCGCA TGC^GCA BamHI 1082 GGATCC G^GATCC BbrPI 4938 CACGTG CAC^GTG BsaAI 4938 CACGTG [CT]AC^GT[GA] BsaBI 2096 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2381 TCCGGA [AT]^CCGG[AT] BseAI 2381 TCCGGA T^CCGGA BspEI 2381 TCCGGA T^CCGGA BstEII 999 GGTAACC G^GT[ACGT]ACC BstPI 999 GGTAACC G^GT[ACGT]ACC BstXI 531 CCAATCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2695 CCTAAGG CC^T[ACGT]AGG DraII 2432 AGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 3279 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 5082 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2695 CCTAAGG CC^T[ACGT]AGG EcoO109I 2432 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 999 GGTAACC G^GT[ACGT]ACC EcoRV 4077 GATATC GAT^ATC FspI 4979 TGCGCA TGC^GCA HindIII 4738 AAGCTT A^AGCTT KpnI 5821 GGTACC GGTAC^C MamI 2096 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2381 TCCGGA T^CCGGA NheI 1073 GCTAGC G^CTAGC PacI 4742 TTAATTAA TTAAT^TAA PmaCI 4938 CACGTG CAC^GTG PmlI 4938 CACGTG CAC^GTG ScaI 5722 AGTACT AGT^ACT SwaI 3647 ATTTAAAT ATTT^AAAT XcmI 5171 CCATCCTTGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 311 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 3111 GTAGAC GT^[AC][GT]AC 4175 GTCTAC AflIII 1402 ACATGT A^C[GA][CT]GT 3512 ACATGT BalI 1180 TGGCCA TGG^CCA 5421 TGGCCA BbsI 2460 GAAGAC GAAGAC(2/6) 5490 GTCTTC BbvCI 1307 CCTCAGC CCTCAGC(-5/-2) 3011 CCTCAGC BcnI 170 CCCGG CC^[GC]GG 3076 CCGGG BglI 51 GCCATGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1037 GCCTGGAGGGC BpuAI 2460 GAAGAC GAAGAC(2/6) 5490 GTCTTC BsaI 3626 GAGACC GGTCTC(1/5) 4099 GGTCTC BseRI 1305 CTCCTC GAGGAG(10/8) 1899 CTCCTC BsmBI 3847 CGTCTC CGTCTC(1/5) 4991 CGTCTC BspLU11I 1402 ACATGT A^CATGT 3512 ACATGT BstAPI 462 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5186 GCAGGTTGTGC EaeI 1180 TGGCCA [CT]^GGCC[GA] 5421 TGGCCA EcoRI 290 GAATTC G^AATTC 4115 GAATTC FauI 227 CCCGC CCCGC(4/6) 232 CCCGC HincII 1056 GTTAAC GT[CT]^[GA]AC 3659 GTTAAC HindII 1056 GTTAAC GT[CT]^[GA]AC 3659 GTTAAC HpaI 1056 GTTAAC GTT^AAC 3659 GTTAAC MluNI 1180 TGGCCA TGG^CCA 5421 TGGCCA MscI 1180 TGGCCA TGG^CCA 5421 TGGCCA NciI 170 CCCGG CC^[GC]GG 3076 CCGGG PflMI 335 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5243 CCAGGGAGTGG PstI 1294 CTGCAG CTGCA^G 4692 CTGCAG SacI 354 GAGCTC GAGCT^C 1063 GAGCTC StuI 2387 AGGCCT AGG^CCT 3425 AGGCCT Van91I 335 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5243 CCAGGGAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AciI AcsI AfaI AflII AluI Alw44I AlwI AlwNI ApaLI ApoI AseI AsnI AspHI AspI AvaII BanI BanII BbvI BfaI BfrI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DsaI EarI EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NsiI NspI PflFI PleI PshBI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XbaI XhoII