##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccII AclI AcyI AgeI
Aor51HI ApaI AscI AvaI AvrII
BbeI BcgI BciVI BclI BglII
BlnI BlpI Bpu1102I BsaHI BsiEI
BsiWI BsoBI Bsp1407I BspDI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I CelII Cfr10I
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I EcoNI FbaI FseI
HaeII HgaI Hin1I KasI KspI
MfeI MluI MunI MvnI NaeI
NarI NdeI NgoMIV NotI NruI
NspV PaeR7I PinAI PmeI PpuMI
PshAI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 5821 GGTACC G^GTACC
AccIII 2381 TCCGGA T^CCGGA
AhdI 5082 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2695 CCTAAGG CC^T[ACGT]AGG
Asp700I 311 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 5821 GGTACC G^GTACC
AspEI 5082 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4979 TGCGCA TGC^GCA
BamHI 1082 GGATCC G^GATCC
BbrPI 4938 CACGTG CAC^GTG
BsaAI 4938 CACGTG [CT]AC^GT[GA]
BsaBI 2096 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2381 TCCGGA [AT]^CCGG[AT]
BseAI 2381 TCCGGA T^CCGGA
BspEI 2381 TCCGGA T^CCGGA
BstEII 999 GGTAACC G^GT[ACGT]ACC
BstPI 999 GGTAACC G^GT[ACGT]ACC
BstXI 531 CCAATCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2695 CCTAAGG CC^T[ACGT]AGG
DraII 2432 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 3279 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 5082 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2695 CCTAAGG CC^T[ACGT]AGG
EcoO109I 2432 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 999 GGTAACC G^GT[ACGT]ACC
EcoRV 4077 GATATC GAT^ATC
FspI 4979 TGCGCA TGC^GCA
HindIII 4738 AAGCTT A^AGCTT
KpnI 5821 GGTACC GGTAC^C
MamI 2096 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2381 TCCGGA T^CCGGA
NheI 1073 GCTAGC G^CTAGC
PacI 4742 TTAATTAA TTAAT^TAA
PmaCI 4938 CACGTG CAC^GTG
PmlI 4938 CACGTG CAC^GTG
ScaI 5722 AGTACT AGT^ACT
SwaI 3647 ATTTAAAT ATTT^AAAT
XcmI 5171 CCATCCTTGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 311 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 3111 GTAGAC GT^[AC][GT]AC
4175 GTCTAC
AflIII 1402 ACATGT A^C[GA][CT]GT
3512 ACATGT
BalI 1180 TGGCCA TGG^CCA
5421 TGGCCA
BbsI 2460 GAAGAC GAAGAC(2/6)
5490 GTCTTC
BbvCI 1307 CCTCAGC CCTCAGC(-5/-2)
3011 CCTCAGC
BcnI 170 CCCGG CC^[GC]GG
3076 CCGGG
BglI 51 GCCATGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1037 GCCTGGAGGGC
BpuAI 2460 GAAGAC GAAGAC(2/6)
5490 GTCTTC
BsaI 3626 GAGACC GGTCTC(1/5)
4099 GGTCTC
BseRI 1305 CTCCTC GAGGAG(10/8)
1899 CTCCTC
BsmBI 3847 CGTCTC CGTCTC(1/5)
4991 CGTCTC
BspLU11I 1402 ACATGT A^CATGT
3512 ACATGT
BstAPI 462 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5186 GCAGGTTGTGC
EaeI 1180 TGGCCA [CT]^GGCC[GA]
5421 TGGCCA
EcoRI 290 GAATTC G^AATTC
4115 GAATTC
FauI 227 CCCGC CCCGC(4/6)
232 CCCGC
HincII 1056 GTTAAC GT[CT]^[GA]AC
3659 GTTAAC
HindII 1056 GTTAAC GT[CT]^[GA]AC
3659 GTTAAC
HpaI 1056 GTTAAC GTT^AAC
3659 GTTAAC
MluNI 1180 TGGCCA TGG^CCA
5421 TGGCCA
MscI 1180 TGGCCA TGG^CCA
5421 TGGCCA
NciI 170 CCCGG CC^[GC]GG
3076 CCGGG
PflMI 335 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5243 CCAGGGAGTGG
PstI 1294 CTGCAG CTGCA^G
4692 CTGCAG
SacI 354 GAGCTC GAGCT^C
1063 GAGCTC
StuI 2387 AGGCCT AGG^CCT
3425 AGGCCT
Van91I 335 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5243 CCAGGGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
Alw44I AlwI AlwNI ApaLI ApoI
AseI AsnI AspHI AspI AvaII
BanI BanII BbvI BfaI BfrI
BmyI BpmI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspHI BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DsaI EarI EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV NsiI NspI
PflFI PleI PshBI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XbaI XhoII