##################################
# NO CUT ENZYMES ( 43 enzymes) #
##################################
Acc65I AclI AflII AgeI AscI
Asp718I BclI BfrI BsiWI BspLU11I
BsrBI BssHII Bst1107I BstEII BstPI
BstZ17I CpoI DrdI EcoO65I EcoRV
FbaI FseI HindIII KpnI MluI
NotI NruI PacI PaeR7I PinAI
PmeI Psp1406I PvuI RsrII SalI
SbfI ScaI SfiI SnaBI Sse8387I
SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 916 TCCGGA T^CCGGA
AhdI 2140 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 5609 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2140 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 913 GGATCC G^GATCC
BbeI 6429 GGCGCC GGCGC^C
BciVI 5482 GGATAC GTATCC(6/5)
BglI 3664 GCCTTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 612 AGATCT A^GATCT
BsaBI 1450 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 916 TCCGGA [AT]^CCGG[AT]
BseAI 916 TCCGGA T^CCGGA
BspEI 916 TCCGGA T^CCGGA
BspHI 5623 TCATGA T^CATGA
BstAPI 1793 GCATCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1389 TTCGAA TT^CGAA
EagI 114 CGGCCG C^GGCCG
Eam1105I 2140 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 114 CGGCCG C^GGCCG
Eco52I 114 CGGCCG C^GGCCG
EcoRI 5385 GAATTC G^AATTC
EcoT22I 5196 ATGCAT ATGCA^T
HpaI 6205 GTTAAC GTT^AAC
KasI 6429 GGCGCC G^GCGCC
KspI 95 CCGCGG CCGC^GG
MamI 1450 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 916 TCCGGA T^CCGGA
NaeI 325 GCCGGC GCC^GGC
NarI 6429 GGCGCC GG^CGCC
NdeI 519 CATATG CA^TATG
NgoMIV 325 GCCGGC G^CCGGC
NsiI 5196 ATGCAT ATGCA^T
NspV 1389 TTCGAA TT^CGAA
PshAI 2062 GACCGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 5623 TCATGA T^CATGA
SacII 95 CCGCGG CCGC^GG
SfoI 6429 GGCGCC GGC^GCC
SfuI 1389 TTCGAA TT^CGAA
SgrAI 2 CACCGGCG C[GA]^CCGG[CT]G
SpeI 5683 ACTAGT A^CTAGT
SphI 5198 GCATGC GCATG^C
XmnI 5609 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 96 CGCG CG^CG
2392 CGCG
Aor51HI 1245 AGCGCT AGC^GCT
1495 AGCGCT
AseI 4419 ATTAAT AT^TAAT
6457 ATTAAT
AsnI 4419 ATTAAT AT^TAAT
6457 ATTAAT
AspI 1096 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1471 GACGGGGTC
AviII 49 TGCGCA TGC^GCA
630 TGCGCA
AvrII 2110 CCTAGG C^CTAGG
3405 CCTAGG
BcgI 1951 CGAAGTGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
6367 CGACCTTTCTGC
BlnI 2110 CCTAGG C^CTAGG
3405 CCTAGG
BsiEI 29 CGACCG CG[GA][CT]^CG
114 CGGCCG
BsmI 1957 GAATGC GAATGC(1/-1)
5146 GAATGC
BspDI 1514 ATCGAT AT^CGAT
4610 ATCGAT
BsrFI 3 ACCGGC [GA]^CCGG[CT]
325 GCCGGC
BstUI 96 CGCG CG^CG
2392 CGCG
Cfr10I 3 ACCGGC [GA]^CCGG[CT]
325 GCCGGC
ClaI 1514 ATCGAT AT^CGAT
4610 ATCGAT
DraIII 1033 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
5467 CACCCTGTG
Eco47III 1245 AGCGCT AGC^GCT
1495 AGCGCT
EcoNI 2080 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5426 CCTCCCAAAGG
FspI 49 TGCGCA TGC^GCA
630 TGCGCA
MfeI 5646 CAATTG C^AATTG
6032 CAATTG
MunI 5646 CAATTG C^AATTG
6032 CAATTG
MvnI 96 CGCG CG^CG
2392 CGCG
NheI 3393 GCTAGC G^CTAGC
4891 GCTAGC
PflFI 1096 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1471 GACGGGGTC
PshBI 4419 ATTAAT AT^TAAT
6457 ATTAAT
SacI 188 GAGCTC GAGCT^C
2753 GAGCTC
SapI 1868 GAAGAGC GCTCTTC(1/4)
2260 GAAGAGC
SexAI 594 ACCTGGT A^CC[AT]GGT
3546 ACCTGGT
SmlI 3693 CTTGAG C^T[CT][GA]AG
5487 CTTGAG
StuI 582 AGGCCT AGG^CCT
2088 AGGCCT
Tth111I 1096 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1471 GACGGGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AatII AccI AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbrPI BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrDI BsrGI
BsrI BssKI BssSI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XcmI XhoII XmaCI
XmaI