################################## # NO CUT ENZYMES ( 43 enzymes) # ################################## Acc65I AclI AflII AgeI AscI Asp718I BclI BfrI BsiWI BspLU11I BsrBI BssHII Bst1107I BstEII BstPI BstZ17I CpoI DrdI EcoO65I EcoRV FbaI FseI HindIII KpnI MluI NotI NruI PacI PaeR7I PinAI PmeI Psp1406I PvuI RsrII SalI SbfI ScaI SfiI SnaBI Sse8387I SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccIII 916 TCCGGA T^CCGGA AhdI 2140 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 5609 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2140 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 913 GGATCC G^GATCC BbeI 6429 GGCGCC GGCGC^C BciVI 5482 GGATAC GTATCC(6/5) BglI 3664 GCCTTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 612 AGATCT A^GATCT BsaBI 1450 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 916 TCCGGA [AT]^CCGG[AT] BseAI 916 TCCGGA T^CCGGA BspEI 916 TCCGGA T^CCGGA BspHI 5623 TCATGA T^CATGA BstAPI 1793 GCATCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1389 TTCGAA TT^CGAA EagI 114 CGGCCG C^GGCCG Eam1105I 2140 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 114 CGGCCG C^GGCCG Eco52I 114 CGGCCG C^GGCCG EcoRI 5385 GAATTC G^AATTC EcoT22I 5196 ATGCAT ATGCA^T HpaI 6205 GTTAAC GTT^AAC KasI 6429 GGCGCC G^GCGCC KspI 95 CCGCGG CCGC^GG MamI 1450 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 916 TCCGGA T^CCGGA NaeI 325 GCCGGC GCC^GGC NarI 6429 GGCGCC GG^CGCC NdeI 519 CATATG CA^TATG NgoMIV 325 GCCGGC G^CCGGC NsiI 5196 ATGCAT ATGCA^T NspV 1389 TTCGAA TT^CGAA PshAI 2062 GACCGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 5623 TCATGA T^CATGA SacII 95 CCGCGG CCGC^GG SfoI 6429 GGCGCC GGC^GCC SfuI 1389 TTCGAA TT^CGAA SgrAI 2 CACCGGCG C[GA]^CCGG[CT]G SpeI 5683 ACTAGT A^CTAGT SphI 5198 GCATGC GCATG^C XmnI 5609 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccII 96 CGCG CG^CG 2392 CGCG Aor51HI 1245 AGCGCT AGC^GCT 1495 AGCGCT AseI 4419 ATTAAT AT^TAAT 6457 ATTAAT AsnI 4419 ATTAAT AT^TAAT 6457 ATTAAT AspI 1096 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1471 GACGGGGTC AviII 49 TGCGCA TGC^GCA 630 TGCGCA AvrII 2110 CCTAGG C^CTAGG 3405 CCTAGG BcgI 1951 CGAAGTGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 6367 CGACCTTTCTGC BlnI 2110 CCTAGG C^CTAGG 3405 CCTAGG BsiEI 29 CGACCG CG[GA][CT]^CG 114 CGGCCG BsmI 1957 GAATGC GAATGC(1/-1) 5146 GAATGC BspDI 1514 ATCGAT AT^CGAT 4610 ATCGAT BsrFI 3 ACCGGC [GA]^CCGG[CT] 325 GCCGGC BstUI 96 CGCG CG^CG 2392 CGCG Cfr10I 3 ACCGGC [GA]^CCGG[CT] 325 GCCGGC ClaI 1514 ATCGAT AT^CGAT 4610 ATCGAT DraIII 1033 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG 5467 CACCCTGTG Eco47III 1245 AGCGCT AGC^GCT 1495 AGCGCT EcoNI 2080 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5426 CCTCCCAAAGG FspI 49 TGCGCA TGC^GCA 630 TGCGCA MfeI 5646 CAATTG C^AATTG 6032 CAATTG MunI 5646 CAATTG C^AATTG 6032 CAATTG MvnI 96 CGCG CG^CG 2392 CGCG NheI 3393 GCTAGC G^CTAGC 4891 GCTAGC PflFI 1096 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1471 GACGGGGTC PshBI 4419 ATTAAT AT^TAAT 6457 ATTAAT SacI 188 GAGCTC GAGCT^C 2753 GAGCTC SapI 1868 GAAGAGC GCTCTTC(1/4) 2260 GAAGAGC SexAI 594 ACCTGGT A^CC[AT]GGT 3546 ACCTGGT SmlI 3693 CTTGAG C^T[CT][GA]AG 5487 CTTGAG StuI 582 AGGCCT AGG^CCT 2088 AGGCCT Tth111I 1096 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1471 GACGGGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 141 enzymes) # ######################################### AatII AccI AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI AspHI AvaI AvaII BalI BanI BanII BbrPI BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BssSI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoII XmaCI XmaI