################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AvrII BbeI BbrPI BciVI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BsiEI BsiWI BspDI BspEI BssHII BssSI Bst1107I BstBI BstZ17I CelII ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRI FseI Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 17 enzymes) # ################################### AviII 4432 TGCGCA TGC^GCA BclI 5856 TGATCA T^GATCA BsaI 6349 GGTCTC GGTCTC(1/5) BsrBI 3933 GAGCGG CCGCTC(-3/-3) BsrFI 6346 GCCGGT [GA]^CCGG[CT] BstEII 402 GGTGACC G^GT[ACGT]ACC BstPI 402 GGTGACC G^GT[ACGT]ACC Cfr10I 6346 GCCGGT [GA]^CCGG[CT] DrdI 5653 GACCCCTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 402 GGTGACC G^GT[ACGT]ACC EcoRV 3917 GATATC GAT^ATC FbaI 5856 TGATCA T^GATCA FspI 4432 TGCGCA TGC^GCA NdeI 3037 CATATG CA^TATG PaeR7I 3348 CTCGAG C^TCGAG PshAI 420 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC XhoI 3348 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccII 1256 CGCG CG^CG 1781 CGCG AflII 1708 CTTAAG C^TTAAG 2082 CTTAAG AflIII 268 ACATGT A^C[GA][CT]GT 5415 ACATGT Alw44I 3211 GTGCAC G^TGCAC 3636 GTGCAC AocI 790 CCTAAGG CC^T[ACGT]AGG 2891 CCTCAGG ApaLI 3211 GTGCAC G^TGCAC 3636 GTGCAC BalI 1210 TGGCCA TGG^CCA 2254 TGGCCA BamHI 4846 GGATCC G^GATCC 5569 GGATCC BanI 3425 GGTGCC G^G[CT][GA]CC 4073 GGTGCC BbvCI 3972 GCTGAGG CCTCAGC(-5/-2) 4137 GCTGAGG BfrI 1708 CTTAAG C^TTAAG 2082 CTTAAG BglII 2999 AGATCT A^GATCT 4925 AGATCT BsmBI 1777 GAGACG CGTCTC(1/5) 4168 GAGACG BspHI 3553 TCATGA T^CATGA 4337 TCATGA BspLU11I 268 ACATGT A^CATGT 5415 ACATGT BspMI 4153 ACCTGC ACCTGC(4/8) 4988 GCAGGT BstUI 1256 CGCG CG^CG 1781 CGCG Bsu36I 790 CCTAAGG CC^T[ACGT]AGG 2891 CCTCAGG DraI 379 TTTAAA TTT^AAA 664 TTTAAA Eco81I 790 CCTAAGG CC^T[ACGT]AGG 2891 CCTCAGG EcoT22I 1035 ATGCAT ATGCA^T 1565 ATGCAT HgaI 1779 GACGC GACGC(5/10) 4840 GCGTC HincII 1219 GTTGAC GT[CT]^[GA]AC 1342 GTCAAC HindII 1219 GTTGAC GT[CT]^[GA]AC 1342 GTCAAC HindIII 559 AAGCTT A^AGCTT 2388 AAGCTT MluNI 1210 TGGCCA TGG^CCA 2254 TGGCCA MscI 1210 TGGCCA TGG^CCA 2254 TGGCCA MvnI 1256 CGCG CG^CG 1781 CGCG NsiI 1035 ATGCAT ATGCA^T 1565 ATGCAT RcaI 3553 TCATGA T^CATGA 4337 TCATGA ScaI 2 AGTACT AGT^ACT 4762 AGTACT SphI 243 GCATGC GCATG^C 1037 GCATGC SspI 1394 AATATT AAT^ATT 2629 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApaI ApoI Asp700I AspHI AspI AvaI AvaII BanII BbsI BbvI BcgI BcnI BfaI BglI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XhoII XmnI