##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AvrII BbeI
BbrPI BciVI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BseAI BsiEI
BsiWI BspDI BspEI BssHII BssSI
Bst1107I BstBI BstZ17I CelII ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoRI FseI Hin1I
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI Van91I XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 17 enzymes) #
###################################
AviII 4432 TGCGCA TGC^GCA
BclI 5856 TGATCA T^GATCA
BsaI 6349 GGTCTC GGTCTC(1/5)
BsrBI 3933 GAGCGG CCGCTC(-3/-3)
BsrFI 6346 GCCGGT [GA]^CCGG[CT]
BstEII 402 GGTGACC G^GT[ACGT]ACC
BstPI 402 GGTGACC G^GT[ACGT]ACC
Cfr10I 6346 GCCGGT [GA]^CCGG[CT]
DrdI 5653 GACCCCTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 402 GGTGACC G^GT[ACGT]ACC
EcoRV 3917 GATATC GAT^ATC
FbaI 5856 TGATCA T^GATCA
FspI 4432 TGCGCA TGC^GCA
NdeI 3037 CATATG CA^TATG
PaeR7I 3348 CTCGAG C^TCGAG
PshAI 420 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
XhoI 3348 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 1256 CGCG CG^CG
1781 CGCG
AflII 1708 CTTAAG C^TTAAG
2082 CTTAAG
AflIII 268 ACATGT A^C[GA][CT]GT
5415 ACATGT
Alw44I 3211 GTGCAC G^TGCAC
3636 GTGCAC
AocI 790 CCTAAGG CC^T[ACGT]AGG
2891 CCTCAGG
ApaLI 3211 GTGCAC G^TGCAC
3636 GTGCAC
BalI 1210 TGGCCA TGG^CCA
2254 TGGCCA
BamHI 4846 GGATCC G^GATCC
5569 GGATCC
BanI 3425 GGTGCC G^G[CT][GA]CC
4073 GGTGCC
BbvCI 3972 GCTGAGG CCTCAGC(-5/-2)
4137 GCTGAGG
BfrI 1708 CTTAAG C^TTAAG
2082 CTTAAG
BglII 2999 AGATCT A^GATCT
4925 AGATCT
BsmBI 1777 GAGACG CGTCTC(1/5)
4168 GAGACG
BspHI 3553 TCATGA T^CATGA
4337 TCATGA
BspLU11I 268 ACATGT A^CATGT
5415 ACATGT
BspMI 4153 ACCTGC ACCTGC(4/8)
4988 GCAGGT
BstUI 1256 CGCG CG^CG
1781 CGCG
Bsu36I 790 CCTAAGG CC^T[ACGT]AGG
2891 CCTCAGG
DraI 379 TTTAAA TTT^AAA
664 TTTAAA
Eco81I 790 CCTAAGG CC^T[ACGT]AGG
2891 CCTCAGG
EcoT22I 1035 ATGCAT ATGCA^T
1565 ATGCAT
HgaI 1779 GACGC GACGC(5/10)
4840 GCGTC
HincII 1219 GTTGAC GT[CT]^[GA]AC
1342 GTCAAC
HindII 1219 GTTGAC GT[CT]^[GA]AC
1342 GTCAAC
HindIII 559 AAGCTT A^AGCTT
2388 AAGCTT
MluNI 1210 TGGCCA TGG^CCA
2254 TGGCCA
MscI 1210 TGGCCA TGG^CCA
2254 TGGCCA
MvnI 1256 CGCG CG^CG
1781 CGCG
NsiI 1035 ATGCAT ATGCA^T
1565 ATGCAT
RcaI 3553 TCATGA T^CATGA
4337 TCATGA
ScaI 2 AGTACT AGT^ACT
4762 AGTACT
SphI 243 GCATGC GCATG^C
1037 GCATGC
SspI 1394 AATATT AAT^ATT
2629 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI Asp700I
AspHI AspI AvaI AvaII BanII
BbsI BbvI BcgI BcnI BfaI
BglI BmyI BpmI BpuAI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BsrDI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XcmI XhoII
XmnI