##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AclI AscI AseI AsnI BbeI
BbrPI BciVI BsiEI BspLU11I BssHII
BstBI BstEII BstPI CpoI EagI
EclXI Eco52I EcoO65I EcoRI EcoRV
FseI HpaI KasI KspI MfeI
MluI MunI NarI NotI NruI
NspV PacI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SfoI SfuI SgrAI
SmaI SpeI Sse8387I SspI SwaI
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 5559 GACGTC GACGT^C
AccI 2234 GTATAC GT^[AC][GT]AC
AccIII 4192 TCCGGA T^CCGGA
AcsI 2637 AAATTT [GA]^AATT[CT]
AcyI 5559 GACGTC G[GA]^CG[CT]C
AflIII 4030 ACGTGT A^C[GA][CT]GT
AgeI 1543 ACCGGT A^CCGGT
AhdI 627 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 2637 AAATTT [GA]^AATT[CT]
AspEI 627 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3728 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 638 TGCGCA TGC^GCA
BcgI 476 CGAGTACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4648 TGATCA T^GATCA
BsaAI 3187 TACGTA [CT]AC^GT[GA]
BsaBI 4289 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 5559 GACGTC G[GA]^CG[CT]C
BseAI 4192 TCCGGA T^CCGGA
BsiWI 5202 CGTACG C^GTACG
BsmI 2311 GAATGC GAATGC(1/-1)
BspDI 2856 ATCGAT AT^CGAT
BspEI 4192 TCCGGA T^CCGGA
Bst1107I 2234 GTATAC GTA^TAC
BstAPI 2837 GCAGGCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2234 GTATAC GTA^TAC
ClaI 2856 ATCGAT AT^CGAT
DraI 5264 TTTAAA TTT^AAA
Eam1105I 627 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 2313 ATGCAT ATGCA^T
FbaI 4648 TGATCA T^GATCA
FspI 638 TGCGCA TGC^GCA
Hin1I 5559 GACGTC G[GA]^CG[CT]C
MamI 4289 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 4192 TCCGGA T^CCGGA
NaeI 4642 GCCGGC GCC^GGC
NdeI 960 CATATG CA^TATG
NgoMIV 4642 GCCGGC G^CCGGC
NsiI 2313 ATGCAT ATGCA^T
PaeR7I 1495 CTCGAG C^TCGAG
PflFI 3728 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1543 ACCGGT A^CCGGT
PshAI 5633 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1013 GAAGAGC GCTCTTC(1/4)
ScaI 478 AGTACT AGT^ACT
SexAI 1243 ACCTGGT A^CC[AT]GGT
SfiI 4952 GGCCAGACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 3187 TACGTA TAC^GTA
SphI 779 GCATGC GCATG^C
Tth111I 3728 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1495 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 3570 CGCG CG^CG
4076 CGCG
Alw44I 3785 GTGCAC G^TGCAC
4119 GTGCAC
Aor51HI 88 AGCGCT AGC^GCT
373 AGCGCT
ApaI 3602 GGGCCC GGGCC^C
5384 GGGCCC
ApaLI 3785 GTGCAC G^TGCAC
4119 GTGCAC
AvrII 4948 CCTAGG C^CTAGG
5498 CCTAGG
BbvCI 2137 CCTCAGC CCTCAGC(-5/-2)
2338 GCTGAGG
BglI 1077 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4953 GCCAGACAGGC
BlnI 4948 CCTAGG C^CTAGG
5498 CCTAGG
BsaWI 1543 ACCGGT [AT]^CCGG[AT]
4192 TCCGGA
Bsp1407I 345 TGTACA T^GTACA
3551 TGTACA
BspMI 2746 ACCTGC ACCTGC(4/8)
2363 GCAGGT
BsrBI 148 CCGCTC CCGCTC(-3/-3)
1596 GAGCGG
BsrFI 1543 ACCGGT [GA]^CCGG[CT]
4642 GCCGGC
BsrGI 345 TGTACA T^GTACA
3551 TGTACA
BssSI 2525 CTCGTG CACGAG(-5/-1)
2554 CTCGTG
BstUI 3570 CGCG CG^CG
4076 CGCG
Cfr10I 1543 ACCGGT [GA]^CCGG[CT]
4642 GCCGGC
DrdI 1907 GACCATGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3159 GACCTGAGGGTC
Eco47III 88 AGCGCT AGC^GCT
373 AGCGCT
HaeII 88 AGCGCT [GA]GCGC^[CT]
373 AGCGCT
HgaI 3728 GACGC GACGC(5/10)
3882 GACGC
HincII 36 GTCAAC GT[CT]^[GA]AC
1833 GTTGAC
HindII 36 GTCAAC GT[CT]^[GA]AC
1833 GTTGAC
MvnI 3570 CGCG CG^CG
4076 CGCG
NheI 1808 GCTAGC G^CTAGC
5054 GCTAGC
PflMI 26 CCACCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5085 CCACACCCTGG
SacI 1498 GAGCTC GAGCT^C
2654 GAGCTC
SspBI 345 TGTACA T^GTACA
3551 TGTACA
Tsp509I 58 AATT ^AATT
2638 AATT
Van91I 26 CCACCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5085 CCACACCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
Acc65I AciI AfaI AflII AluI
AlwI AlwNI AocI Asp700I Asp718I
AspHI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvI BcnI
BfaI BfrI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspHI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I TspRI
TthHB8I XcmI XhoII XmnI