##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
Acc65I AflII Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AviII
AvrII BamHI BclI BfrI BlnI
BsmI BssHII Bst1107I BstBI BstZ17I
Eco47III EcoRI EcoT22I FbaI FseI
FspI HindIII HpaI KpnI MfeI
MluI MunI NaeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PmeI PshAI PshBI PvuI SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 1589 GACGTC GACGT^C
AccIII 570 TCCGGA T^CCGGA
AclI 1338 AACGTT AA^CGTT
AcsI 3019 GAATTT [GA]^AATT[CT]
AgeI 5379 ACCGGT A^CCGGT
AocI 3753 CCTAAGG CC^T[ACGT]AGG
ApoI 3019 GAATTT [GA]^AATT[CT]
AspI 455 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 2998 GCAGACAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 3772 GCCAAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 2582 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 570 TCCGGA T^CCGGA
BsiWI 2463 CGTACG C^GTACG
Bsp1407I 4901 TGTACA T^GTACA
BspDI 971 ATCGAT AT^CGAT
BspEI 570 TCCGGA T^CCGGA
BspLU11I 3659 ACATGT A^CATGT
BsrBI 1156 CCGCTC CCGCTC(-3/-3)
BsrDI 5944 GCAATG GCAATG(2/0)
BsrGI 4901 TGTACA T^GTACA
BstAPI 3988 GCAGTAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2263 CCAGGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3753 CCTAAGG CC^T[ACGT]AGG
ClaI 971 ATCGAT AT^CGAT
CpoI 43 CGGTCCG CG^G[AT]CCG
DraI 4184 TTTAAA TTT^AAA
DrdI 4754 GACAAGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 433 CGGCCG C^GGCCG
EclXI 433 CGGCCG C^GGCCG
Eco52I 433 CGGCCG C^GGCCG
Eco81I 3753 CCTAAGG CC^T[ACGT]AGG
KspI 2475 CCGCGG CCGC^GG
MamI 2582 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 570 TCCGGA T^CCGGA
PaeR7I 2105 CTCGAG C^TCGAG
PflFI 455 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 5379 ACCGGT A^CCGGT
Psp1406I 1338 AACGTT AA^CGTT
RsrII 43 CGGTCCG CG^G[AT]CCG
SacI 800 GAGCTC GAGCT^C
SacII 2475 CCGCGG CCGC^GG
SspBI 4901 TGTACA T^GTACA
SspI 3676 AATATT AAT^ATT
Tth111I 455 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5899 TCTAGA T^CTAGA
XhoI 2105 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 2476 CGCG CG^CG
2915 CGCG
AhdI 1457 GACGCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2830 GACGTACTGTC
ApaI 5 GGGCCC GGGCC^C
2072 GGGCCC
Asp700I 2290 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5794 GAATCTTTTC
AspEI 1457 GACGCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2830 GACGTACTGTC
BbeI 1976 GGCGCC GGCGC^C
2468 GGCGCC
BbrPI 779 CACGTG CAC^GTG
1803 CACGTG
BciVI 3749 GTATCC GTATCC(6/5)
4613 GTATCC
BglII 1062 AGATCT A^GATCT
3765 AGATCT
BsiEI 433 CGGCCG CG[GA][CT]^CG
1315 CGACCG
BspHI 2298 TCATGA T^CATGA
3599 TCATGA
BstUI 2476 CGCG CG^CG
2915 CGCG
DraIII 3579 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
5671 CACCGTGTG
Eam1105I 1457 GACGCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2830 GACGTACTGTC
EcoRV 974 GATATC GAT^ATC
5903 GATATC
HaeII 1976 GGCGCC [GA]GCGC^[CT]
2468 GGCGCC
HincII 938 GTTGAC GT[CT]^[GA]AC
4670 GTCAAC
HindII 938 GTTGAC GT[CT]^[GA]AC
4670 GTCAAC
KasI 1976 GGCGCC G^GCGCC
2468 GGCGCC
MvnI 2476 CGCG CG^CG
2915 CGCG
NarI 1976 GGCGCC GG^CGCC
2468 GGCGCC
NdeI 376 CATATG CA^TATG
5012 CATATG
PmaCI 779 CACGTG CAC^GTG
1803 CACGTG
PmlI 779 CACGTG CAC^GTG
1803 CACGTG
RcaI 2298 TCATGA T^CATGA
3599 TCATGA
SexAI 456 ACCTGGT A^CC[AT]GGT
4236 ACCTGGT
SfoI 1976 GGCGCC GGC^GCC
2468 GGCGCC
SphI 718 GCATGC GCATG^C
5136 GCATGC
StuI 248 AGGCCT AGG^CCT
5036 AGGCCT
XcmI 1233 CCAAGGGCTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1920 CCACGTCCAGCCTGG
XmnI 2290 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5794 GAATCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AciI AcyI AfaI AflIII
AluI AlwI AlwNI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BssSI BstEII
BstF5I BstNI BstPI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI