################################## # NO CUT ENZYMES ( 55 enzymes) # ################################## Acc65I AflII Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AviII AvrII BamHI BclI BfrI BlnI BsmI BssHII Bst1107I BstBI BstZ17I Eco47III EcoRI EcoT22I FbaI FseI FspI HindIII HpaI KpnI MfeI MluI MunI NaeI NgoMIV NheI NotI NruI NsiI NspV PacI PmeI PshAI PshBI PvuI SalI SapI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 1589 GACGTC GACGT^C AccIII 570 TCCGGA T^CCGGA AclI 1338 AACGTT AA^CGTT AcsI 3019 GAATTT [GA]^AATT[CT] AgeI 5379 ACCGGT A^CCGGT AocI 3753 CCTAAGG CC^T[ACGT]AGG ApoI 3019 GAATTT [GA]^AATT[CT] AspI 455 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BcgI 2998 GCAGACAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 3772 GCCAAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 2582 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 570 TCCGGA T^CCGGA BsiWI 2463 CGTACG C^GTACG Bsp1407I 4901 TGTACA T^GTACA BspDI 971 ATCGAT AT^CGAT BspEI 570 TCCGGA T^CCGGA BspLU11I 3659 ACATGT A^CATGT BsrBI 1156 CCGCTC CCGCTC(-3/-3) BsrDI 5944 GCAATG GCAATG(2/0) BsrGI 4901 TGTACA T^GTACA BstAPI 3988 GCAGTAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 2263 CCAGGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 3753 CCTAAGG CC^T[ACGT]AGG ClaI 971 ATCGAT AT^CGAT CpoI 43 CGGTCCG CG^G[AT]CCG DraI 4184 TTTAAA TTT^AAA DrdI 4754 GACAAGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 433 CGGCCG C^GGCCG EclXI 433 CGGCCG C^GGCCG Eco52I 433 CGGCCG C^GGCCG Eco81I 3753 CCTAAGG CC^T[ACGT]AGG KspI 2475 CCGCGG CCGC^GG MamI 2582 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 570 TCCGGA T^CCGGA PaeR7I 2105 CTCGAG C^TCGAG PflFI 455 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 5379 ACCGGT A^CCGGT Psp1406I 1338 AACGTT AA^CGTT RsrII 43 CGGTCCG CG^G[AT]CCG SacI 800 GAGCTC GAGCT^C SacII 2475 CCGCGG CCGC^GG SspBI 4901 TGTACA T^GTACA SspI 3676 AATATT AAT^ATT Tth111I 455 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 5899 TCTAGA T^CTAGA XhoI 2105 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccII 2476 CGCG CG^CG 2915 CGCG AhdI 1457 GACGCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2830 GACGTACTGTC ApaI 5 GGGCCC GGGCC^C 2072 GGGCCC Asp700I 2290 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5794 GAATCTTTTC AspEI 1457 GACGCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2830 GACGTACTGTC BbeI 1976 GGCGCC GGCGC^C 2468 GGCGCC BbrPI 779 CACGTG CAC^GTG 1803 CACGTG BciVI 3749 GTATCC GTATCC(6/5) 4613 GTATCC BglII 1062 AGATCT A^GATCT 3765 AGATCT BsiEI 433 CGGCCG CG[GA][CT]^CG 1315 CGACCG BspHI 2298 TCATGA T^CATGA 3599 TCATGA BstUI 2476 CGCG CG^CG 2915 CGCG DraIII 3579 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG 5671 CACCGTGTG Eam1105I 1457 GACGCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2830 GACGTACTGTC EcoRV 974 GATATC GAT^ATC 5903 GATATC HaeII 1976 GGCGCC [GA]GCGC^[CT] 2468 GGCGCC HincII 938 GTTGAC GT[CT]^[GA]AC 4670 GTCAAC HindII 938 GTTGAC GT[CT]^[GA]AC 4670 GTCAAC KasI 1976 GGCGCC G^GCGCC 2468 GGCGCC MvnI 2476 CGCG CG^CG 2915 CGCG NarI 1976 GGCGCC GG^CGCC 2468 GGCGCC NdeI 376 CATATG CA^TATG 5012 CATATG PmaCI 779 CACGTG CAC^GTG 1803 CACGTG PmlI 779 CACGTG CAC^GTG 1803 CACGTG RcaI 2298 TCATGA T^CATGA 3599 TCATGA SexAI 456 ACCTGGT A^CC[AT]GGT 4236 ACCTGGT SfoI 1976 GGCGCC GGC^GCC 2468 GGCGCC SphI 718 GCATGC GCATG^C 5136 GCATGC StuI 248 AGGCCT AGG^CCT 5036 AGGCCT XcmI 1233 CCAAGGGCTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1920 CCACGTCCAGCCTGG XmnI 2290 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5794 GAATCTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AccI AciI AcyI AfaI AflIII AluI AlwI AlwNI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstEII BstF5I BstNI BstPI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI