##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AclI AgeI AhdI Aor51HI AscI
Asp700I AspEI AspI AvrII BbvCI
BclI BglII BlnI BsiWI BspDI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI Eam1105I Eco47III EcoO65I EcoRV
FbaI FseI NdeI NheI NotI
NruI NspV PflFI PinAI PmeI
PshAI Psp1406I PvuI SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI Sse8387I SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 2963 GTCTAC GT^[AC][GT]AC
AccIII 230 TCCGGA T^CCGGA
Alw44I 4104 GTGCAC G^TGCAC
ApaLI 4104 GTGCAC G^TGCAC
AseI 497 ATTAAT AT^TAAT
AsnI 497 ATTAAT AT^TAAT
BbrPI 272 CACGTG CAC^GTG
BcgI 364 GCAGCAGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1684 GTATCC GTATCC(6/5)
BsaAI 272 CACGTG [CT]AC^GT[GA]
BsaBI 222 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 230 TCCGGA T^CCGGA
BsiEI 146 CGGCCG CG[GA][CT]^CG
BsmBI 731 CGTCTC CGTCTC(1/5)
BsmI 5368 GCATTC GAATGC(1/-1)
BspEI 230 TCCGGA T^CCGGA
BspLU11I 479 ACATGT A^CATGT
BssSI 2728 CTCGTG CACGAG(-5/-1)
CpoI 281 CGGTCCG CG^G[AT]CCG
DrdI 723 GACGTCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 146 CGGCCG C^GGCCG
EclXI 146 CGGCCG C^GGCCG
Eco52I 146 CGGCCG C^GGCCG
EcoRI 3640 GAATTC G^AATTC
HindIII 4671 AAGCTT A^AGCTT
HpaI 954 GTTAAC GTT^AAC
KspI 278 CCGCGG CCGC^GG
MamI 222 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5146 CAATTG C^AATTG
MluI 2512 ACGCGT A^CGCGT
MroI 230 TCCGGA T^CCGGA
MunI 5146 CAATTG C^AATTG
NaeI 1560 GCCGGC GCC^GGC
NgoMIV 1560 GCCGGC G^CCGGC
PacI 3558 TTAATTAA TTAAT^TAA
PmaCI 272 CACGTG CAC^GTG
PmlI 272 CACGTG CAC^GTG
PshBI 497 ATTAAT AT^TAAT
RsrII 281 CGGTCCG CG^G[AT]CCG
SacI 895 GAGCTC GAGCT^C
SacII 278 CCGCGG CCGC^GG
SapI 4838 GAAGAGC GCTCTTC(1/4)
SpeI 3431 ACTAGT A^CTAGT
SphI 4461 GCATGC GCATG^C
SspI 5650 AATATT AAT^ATT
XbaI 5625 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 723 GACGTC GACGT^C
1092 GACGTC
Acc65I 865 GGTACC G^GTACC
1630 GGTACC
AcsI 1371 AAATTC [GA]^AATT[CT]
3640 GAATTC
AflII 48 CTTAAG C^TTAAG
2951 CTTAAG
AlwNI 241 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
3393 CAGCTTCTG
ApoI 1371 AAATTC [GA]^AATT[CT]
3640 GAATTC
Asp718I 865 GGTACC G^GTACC
1630 GGTACC
BfrI 48 CTTAAG C^TTAAG
2951 CTTAAG
BglI 1107 GCCATTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3305 GCCTTCAAGGC
BsaWI 230 TCCGGA [AT]^CCGG[AT]
1456 ACCGGA
BspHI 1918 TCATGA T^CATGA
5452 TCATGA
BssHII 57 GCGCGC G^CGCGC
96 GCGCGC
EcoT22I 2339 ATGCAT ATGCA^T
5636 ATGCAT
KpnI 865 GGTACC GGTAC^C
1630 GGTACC
NcoI 1499 CCATGG C^CATGG
3778 CCATGG
NsiI 2339 ATGCAT ATGCA^T
5636 ATGCAT
PaeR7I 2524 CTCGAG C^TCGAG
3503 CTCGAG
PvuII 921 CAGCTG CAG^CTG
2060 CAGCTG
RcaI 1918 TCATGA T^CATGA
5452 TCATGA
SmaI 207 CCCGGG CCC^GGG
598 CCCGGG
StuI 1939 AGGCCT AGG^CCT
4029 AGGCCT
XhoI 2524 CTCGAG C^TCGAG
3503 CTCGAG
XmaCI 207 CCCGGG C^CCGGG
598 CCCGGG
XmaI 207 CCCGGG C^CCGGG
598 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AocI ApaI AspHI
AvaI AvaII AviII BalI BamHI
BanI BanII BbeI BbsI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII