##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Aor51HI AscI AseI AsnI Asp718I
AviII BclI BglI BsaWI BseAI
BsiEI BsiWI BspEI BssHII BssSI
BstBI CpoI DrdI EagI EclXI
Eco47III Eco52I EcoRV FbaI FseI
FspI HpaI KpnI KspI MluI
MroI NdeI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SfiI SfuI SgrAI SnaBI
SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccII 6 CGCG CG^CG
AflII 5146 CTTAAG C^TTAAG
AhdI 4328 GACATGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 496 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4328 GACATGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 5316 CCCGGG C^[CT]CG[GA]G
BbrPI 521 CACGTG CAC^GTG
BcgI 3595 GCATTTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 5146 CTTAAG C^TTAAG
BsaAI 521 CACGTG [CT]AC^GT[GA]
BsaBI 714 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 5316 CCCGGG C^[CT]CG[GA]G
BspDI 544 ATCGAT AT^CGAT
BsrDI 798 CATTGC GCAATG(2/0)
Bst1107I 2514 GTATAC GTA^TAC
BstAPI 4621 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 562 GGTTACC G^GT[ACGT]ACC
BstPI 562 GGTTACC G^GT[ACGT]ACC
BstUI 6 CGCG CG^CG
BstZ17I 2514 GTATAC GTA^TAC
ClaI 544 ATCGAT AT^CGAT
Eam1105I 4328 GACATGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 562 GGTTACC G^GT[ACGT]ACC
MamI 714 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3552 CAATTG C^AATTG
MunI 3552 CAATTG C^AATTG
MvnI 6 CGCG CG^CG
NaeI 73 GCCGGC GCC^GGC
NgoMIV 73 GCCGGC G^CCGGC
PmaCI 521 CACGTG CAC^GTG
PmlI 521 CACGTG CAC^GTG
PshAI 3523 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 894 CCTGCAGG CCTGCA^GG
SmaI 5316 CCCGGG CCC^GGG
Sse8387I 894 CCTGCAGG CCTGCA^GG
XbaI 1620 TCTAGA T^CTAGA
XmaCI 5316 CCCGGG C^CCGGG
XmaI 5316 CCCGGG C^CCGGG
XmnI 496 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 2514 GTATAC GT^[AC][GT]AC
5243 GTAGAC
ApaI 1118 GGGCCC GGGCC^C
2724 GGGCCC
AvrII 1976 CCTAGG C^CTAGG
2608 CCTAGG
BamHI 2220 GGATCC G^GATCC
4770 GGATCC
BbvCI 321 GCTGAGG CCTCAGC(-5/-2)
2565 GCTGAGG
BglII 236 AGATCT A^GATCT
1867 AGATCT
BlnI 1976 CCTAGG C^CTAGG
2608 CCTAGG
BsgI 1738 CTGCAC GTGCAG(16/14)
2637 GTGCAG
BspHI 405 TCATGA T^CATGA
533 TCATGA
BspLU11I 3295 ACATGT A^CATGT
3705 ACATGT
BsrBI 5202 CCGCTC CCGCTC(-3/-3)
52 GAGCGG
BsrFI 73 GCCGGC [GA]^CCGG[CT]
810 GCCGGT
Cfr10I 73 GCCGGC [GA]^CCGG[CT]
810 GCCGGT
DraIII 902 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
4925 CACTGGGTG
EcoNI 2224 CCTCCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2539 CCTGGGGTAGG
EcoT22I 2910 ATGCAT ATGCA^T
5605 ATGCAT
HindIII 1762 AAGCTT A^AGCTT
2599 AAGCTT
NheI 2078 GCTAGC G^CTAGC
3032 GCTAGC
NsiI 2910 ATGCAT ATGCA^T
5605 ATGCAT
PflMI 3688 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5289 CCAAGTGCTGG
RcaI 405 TCATGA T^CATGA
533 TCATGA
SacI 529 GAGCTC GAGCT^C
2765 GAGCTC
SexAI 972 ACCTGGT A^CC[AT]GGT
1809 ACCTGGT
SpeI 1296 ACTAGT A^CTAGT
2510 ACTAGT
StuI 1053 AGGCCT AGG^CCT
3186 AGGCCT
Van91I 3688 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5289 CCAAGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI AspHI AspI AvaII
BalI BanI BanII BbeI BbsI
BbvI BciVI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
Bsp1286I Bsp1407I BspMI BsrGI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PflFI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SfoI
SmlI SphI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XcmI XhoII