##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AcyI AhdI
Alw44I ApaLI AscI Asp718I AspEI
AviII AvrII BamHI BbeI BclI
BlnI BsaHI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspHI BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco52I
EcoRI FbaI FseI FspI Hin1I
KasI KpnI KspI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NheI NotI NspV PacI PaeR7I
PmeI PshAI PvuI RcaI RsrII
SacI SacII SalI SapI ScaI
SfoI SfuI SgrAI SnaBI SpeI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccI 3581 GTCTAC GT^[AC][GT]AC
AclI 1234 AACGTT AA^CGTT
AflII 3172 CTTAAG C^TTAAG
AgeI 1133 ACCGGT A^CCGGT
AocI 2549 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1996 AGCGCT AGC^GCT
ApaI 454 GGGCCC GGGCC^C
AseI 3233 ATTAAT AT^TAAT
AsnI 3233 ATTAAT AT^TAAT
AspI 2596 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 985 GCTGAGG CCTCAGC(-5/-2)
BcgI 1450 CGAGGTAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2111 GGATAC GTATCC(6/5)
BfrI 3172 CTTAAG C^TTAAG
BsaBI 4457 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 3309 TGTACA T^GTACA
BspLU11I 3312 ACATGT A^CATGT
BsrBI 4322 GAGCGG CCGCTC(-3/-3)
BsrDI 3676 GCAATG GCAATG(2/0)
BsrFI 1133 ACCGGT [GA]^CCGG[CT]
BsrGI 3309 TGTACA T^GTACA
BssHII 2393 GCGCGC G^CGCGC
BstAPI 2958 GCAGTTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2119 GGTCACC G^GT[ACGT]ACC
BstPI 2119 GGTCACC G^GT[ACGT]ACC
Bsu36I 2549 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1133 ACCGGT [GA]^CCGG[CT]
DraIII 4598 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 2465 CTCTTC CTCTTC(1/4)
Eco47III 1996 AGCGCT AGC^GCT
Eco81I 2549 CCTGAGG CC^T[ACGT]AGG
EcoNI 2866 CCTTTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2119 GGTCACC G^GT[ACGT]ACC
EcoT22I 2965 ATGCAT ATGCA^T
HincII 991 GTTAAC GT[CT]^[GA]AC
HindII 991 GTTAAC GT[CT]^[GA]AC
HindIII 2769 AAGCTT A^AGCTT
HpaI 991 GTTAAC GTT^AAC
Ksp632I 2465 CTCTTC CTCTTC(1/4)
MamI 4457 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 363 CCATGG C^CATGG
NdeI 323 CATATG CA^TATG
NruI 2143 TCGCGA TCG^CGA
NsiI 2965 ATGCAT ATGCA^T
PflFI 2596 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1133 ACCGGT A^CCGGT
PpuMI 2594 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 3233 ATTAAT AT^TAAT
Psp1406I 1234 AACGTT AA^CGTT
SbfI 1933 CCTGCAGG CCTGCA^GG
SexAI 2115 ACCTGGT A^CC[AT]GGT
SmaI 2198 CCCGGG CCC^GGG
Sse8387I 1933 CCTGCAGG CCTGCA^GG
SspBI 3309 TGTACA T^GTACA
Tth111I 2596 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2692 TCTAGA T^CTAGA
XmaCI 2198 CCCGGG C^CCGGG
XmaI 2198 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 1791 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
4002 CAGTTCCTG
AvaI 1118 CCCGAG C^[CT]CG[GA]G
2198 CCCGGG
BalI 2152 TGGCCA TGG^CCA
2566 TGGCCA
BanI 1775 GGCACC G^G[CT][GA]CC
4327 GGTGCC
BbsI 142 GAAGAC GAAGAC(2/6)
2458 GTCTTC
BglI 1931 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2516 GCCAGGTCGGC
BpmI 606 CTGGAG CTGGAG(16/14)
950 CTGGAG
BpuAI 142 GAAGAC GAAGAC(2/6)
2458 GTCTTC
BsaWI 754 TCCGGT [AT]^CCGG[AT]
1133 ACCGGT
BsoBI 1118 CCCGAG C^[CT]CG[GA]G
2198 CCCGGG
DsaI 363 CCATGG C^C[GA][CT]GG
1326 CCACGG
EcoRV 538 GATATC GAT^ATC
1316 GATATC
MluNI 2152 TGGCCA TGG^CCA
2566 TGGCCA
MscI 2152 TGGCCA TGG^CCA
2566 TGGCCA
MspA1I 1853 CAGCTG C[AC]G^C[GT]G
1898 CAGCTG
PvuII 1853 CAGCTG CAG^CTG
1898 CAGCTG
SfiI 1930 GGCCCTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2515 GGCCAGGTCGGCC
SmlI 2183 CTCAAG C^T[CT][GA]AG
3172 CTTAAG
SphI 3064 GCATGC GCATG^C
4292 GCATGC
StuI 1938 AGGCCT AGG^CCT
3621 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI Asp700I AspHI
AvaII BanII BbrPI BbvI BcnI
BfaI BglII BlpI BmyI Bpu1102I
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI