##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccIII AclI AgeI Aor51HI
ApaI AscI AspI AviII BbrPI
BcgI BglI BsaAI BseAI BsiEI
BsiWI BspEI BsrBI BsrFI BssHII
BstBI Cfr10I CpoI DrdI EagI
EclXI Eco47III Eco52I FseI FspI
MfeI MluI MroI MunI NaeI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI Sse8387I SwaI
Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 2487 GGCGCC G[GA]^CG[CT]C
AflII 4541 CTTAAG C^TTAAG
Alw44I 3592 GTGCAC G^TGCAC
AocI 875 CCTGAGG CC^T[ACGT]AGG
ApaLI 3592 GTGCAC G^TGCAC
BamHI 6200 GGATCC G^GATCC
BbeI 2487 GGCGCC GGCGC^C
BbvCI 1175 GCTGAGG CCTCAGC(-5/-2)
BclI 5854 TGATCA T^GATCA
BfrI 4541 CTTAAG C^TTAAG
BsaBI 4927 GATCGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2487 GGCGCC G[GA]^CG[CT]C
BsmBI 1833 CGTCTC CGTCTC(1/5)
BsmI 2958 GAATGC GAATGC(1/-1)
BspDI 4928 ATCGAT AT^CGAT
BssSI 2772 CACGAG CACGAG(-5/-1)
Bst1107I 4276 GTATAC GTA^TAC
BstEII 763 GGTCACC G^GT[ACGT]ACC
BstPI 763 GGTCACC G^GT[ACGT]ACC
BstXI 1855 CCATGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4276 GTATAC GTA^TAC
Bsu36I 875 CCTGAGG CC^T[ACGT]AGG
ClaI 4928 ATCGAT AT^CGAT
DraIII 2382 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 875 CCTGAGG CC^T[ACGT]AGG
EcoO65I 763 GGTCACC G^GT[ACGT]ACC
EcoT22I 3542 ATGCAT ATGCA^T
FbaI 5854 TGATCA T^GATCA
HaeII 2487 GGCGCC [GA]GCGC^[CT]
Hin1I 2487 GGCGCC G[GA]^CG[CT]C
HincII 3690 GTTAAC GT[CT]^[GA]AC
HindII 3690 GTTAAC GT[CT]^[GA]AC
HpaI 3690 GTTAAC GTT^AAC
KasI 2487 GGCGCC G^GCGCC
KspI 912 CCGCGG CCGC^GG
MamI 4927 GATCGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 2487 GGCGCC GG^CGCC
NsiI 3542 ATGCAT ATGCA^T
PshAI 3334 GACTAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 912 CCGCGG CCGC^GG
SfoI 2487 GGCGCC GGC^GCC
SphI 3540 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 685 GGTACC G^GTACC
4759 GGTACC
Asp718I 685 GGTACC G^GTACC
4759 GGTACC
AvaI 315 CTCGAG C^[CT]CG[GA]G
2053 CTCGAG
AvrII 2651 CCTAGG C^CTAGG
4331 CCTAGG
BcnI 1780 CCCGG CC^[GC]GG
2119 CCGGG
BglII 5456 AGATCT A^GATCT
5542 AGATCT
BlnI 2651 CCTAGG C^CTAGG
4331 CCTAGG
BlpI 350 GCTCAGC GC^T[ACGT]AGC
7267 GCTCAGC
Bpu1102I 350 GCTCAGC GC^T[ACGT]AGC
7267 GCTCAGC
BsaI 2647 GAGACC GGTCTC(1/5)
1150 GGTCTC
BsaWI 126 ACCGGA [AT]^CCGG[AT]
1751 TCCGGT
BsoBI 315 CTCGAG C^[CT]CG[GA]G
2053 CTCGAG
CelII 350 GCTCAGC GC^T[ACGT]AGC
7267 GCTCAGC
EcoNI 4200 CCTTTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4525 CCTCTCAAAGG
EcoRI 6109 GAATTC G^AATTC
6685 GAATTC
HgaI 1832 GCGTC GACGC(5/10)
5895 GCGTC
KpnI 685 GGTACC GGTAC^C
4759 GGTACC
MaeII 2599 ACGT A^CGT
6358 ACGT
NciI 1780 CCCGG CC^[GC]GG
2119 CCGGG
NdeI 4754 CATATG CA^TATG
5958 CATATG
PaeR7I 315 CTCGAG C^TCGAG
2053 CTCGAG
SacI 2141 GAGCTC GAGCT^C
5433 GAGCTC
SpeI 2548 ACTAGT A^CTAGT
4974 ACTAGT
XcmI 42 CCAGCAGCAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1126 CCAAGCTGAAAGTGG
XhoI 315 CTCGAG C^TCGAG
2053 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AhdI AluI AlwI AlwNI
ApoI AseI AsnI Asp700I AspEI
AspHI AvaII BalI BanI BanII
BbsI BbvI BciVI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspHI BspLU11I BspMI
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI Eam1105I EarI
EcoO109I EcoRII EcoRV EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PshBI PstI PvuII RcaI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XhoII XmnI