################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## AatII AccIII AclI AgeI Aor51HI ApaI AscI AspI AviII BbrPI BcgI BglI BsaAI BseAI BsiEI BsiWI BspEI BsrBI BsrFI BssHII BstBI Cfr10I CpoI DrdI EagI EclXI Eco47III Eco52I FseI FspI MfeI MluI MroI MunI NaeI NgoMIV NheI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI Sse8387I SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcyI 2487 GGCGCC G[GA]^CG[CT]C AflII 4541 CTTAAG C^TTAAG Alw44I 3592 GTGCAC G^TGCAC AocI 875 CCTGAGG CC^T[ACGT]AGG ApaLI 3592 GTGCAC G^TGCAC BamHI 6200 GGATCC G^GATCC BbeI 2487 GGCGCC GGCGC^C BbvCI 1175 GCTGAGG CCTCAGC(-5/-2) BclI 5854 TGATCA T^GATCA BfrI 4541 CTTAAG C^TTAAG BsaBI 4927 GATCGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2487 GGCGCC G[GA]^CG[CT]C BsmBI 1833 CGTCTC CGTCTC(1/5) BsmI 2958 GAATGC GAATGC(1/-1) BspDI 4928 ATCGAT AT^CGAT BssSI 2772 CACGAG CACGAG(-5/-1) Bst1107I 4276 GTATAC GTA^TAC BstEII 763 GGTCACC G^GT[ACGT]ACC BstPI 763 GGTCACC G^GT[ACGT]ACC BstXI 1855 CCATGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 4276 GTATAC GTA^TAC Bsu36I 875 CCTGAGG CC^T[ACGT]AGG ClaI 4928 ATCGAT AT^CGAT DraIII 2382 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 875 CCTGAGG CC^T[ACGT]AGG EcoO65I 763 GGTCACC G^GT[ACGT]ACC EcoT22I 3542 ATGCAT ATGCA^T FbaI 5854 TGATCA T^GATCA HaeII 2487 GGCGCC [GA]GCGC^[CT] Hin1I 2487 GGCGCC G[GA]^CG[CT]C HincII 3690 GTTAAC GT[CT]^[GA]AC HindII 3690 GTTAAC GT[CT]^[GA]AC HpaI 3690 GTTAAC GTT^AAC KasI 2487 GGCGCC G^GCGCC KspI 912 CCGCGG CCGC^GG MamI 4927 GATCGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 2487 GGCGCC GG^CGCC NsiI 3542 ATGCAT ATGCA^T PshAI 3334 GACTAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 912 CCGCGG CCGC^GG SfoI 2487 GGCGCC GGC^GCC SphI 3540 GCATGC GCATG^C ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Acc65I 685 GGTACC G^GTACC 4759 GGTACC Asp718I 685 GGTACC G^GTACC 4759 GGTACC AvaI 315 CTCGAG C^[CT]CG[GA]G 2053 CTCGAG AvrII 2651 CCTAGG C^CTAGG 4331 CCTAGG BcnI 1780 CCCGG CC^[GC]GG 2119 CCGGG BglII 5456 AGATCT A^GATCT 5542 AGATCT BlnI 2651 CCTAGG C^CTAGG 4331 CCTAGG BlpI 350 GCTCAGC GC^T[ACGT]AGC 7267 GCTCAGC Bpu1102I 350 GCTCAGC GC^T[ACGT]AGC 7267 GCTCAGC BsaI 2647 GAGACC GGTCTC(1/5) 1150 GGTCTC BsaWI 126 ACCGGA [AT]^CCGG[AT] 1751 TCCGGT BsoBI 315 CTCGAG C^[CT]CG[GA]G 2053 CTCGAG CelII 350 GCTCAGC GC^T[ACGT]AGC 7267 GCTCAGC EcoNI 4200 CCTTTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4525 CCTCTCAAAGG EcoRI 6109 GAATTC G^AATTC 6685 GAATTC HgaI 1832 GCGTC GACGC(5/10) 5895 GCGTC KpnI 685 GGTACC GGTAC^C 4759 GGTACC MaeII 2599 ACGT A^CGT 6358 ACGT NciI 1780 CCCGG CC^[GC]GG 2119 CCGGG NdeI 4754 CATATG CA^TATG 5958 CATATG PaeR7I 315 CTCGAG C^TCGAG 2053 CTCGAG SacI 2141 GAGCTC GAGCT^C 5433 GAGCTC SpeI 2548 ACTAGT A^CTAGT 4974 ACTAGT XcmI 42 CCAGCAGCAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1126 CCAAGCTGAAAGTGG XhoI 315 CTCGAG C^TCGAG 2053 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 128 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AhdI AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspEI AspHI AvaII BalI BanI BanII BbsI BbvI BciVI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspHI BspLU11I BspMI BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI Eam1105I EarI EcoO109I EcoRII EcoRV EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PshBI PstI PvuII RcaI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XhoII XmnI