################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AccIII AflII Aor51HI AscI AseI AsnI AviII BbeI BbrPI BcgI BfrI BglI BglII BsaAI BsaBI BseAI BsiWI BsmBI BspDI BspEI BspHI BssHII BstBI BstEII BstPI ClaI CpoI DraIII DrdI Eco47III EcoO65I FseI FspI HaeII HpaI KasI KspI MamI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NspV PacI PaeR7I PmaCI PmeI PmlI PshBI PvuI RcaI RsrII SacII SalI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SspI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 1248 GACGTC GACGT^C Acc65I 6032 GGTACC G^GTACC AccII 1048 CGCG CG^CG AgeI 1112 ACCGGT A^CCGGT ApaI 4486 GGGCCC GGGCC^C Asp700I 409 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 6032 GGTACC G^GTACC AvaI 2178 CTCGGG C^[CT]CG[GA]G AvrII 6028 CCTAGG C^CTAGG BclI 3097 TGATCA T^GATCA BlnI 6028 CCTAGG C^CTAGG BsmI 191 GCATTC GAATGC(1/-1) BsoBI 2178 CTCGGG C^[CT]CG[GA]G BssSI 2890 CACGAG CACGAG(-5/-1) Bst1107I 4653 GTATAC GTA^TAC BstAPI 656 GCAGTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 1048 CGCG CG^CG BstZ17I 4653 GTATAC GTA^TAC EagI 269 CGGCCG C^GGCCG EclXI 269 CGGCCG C^GGCCG Eco52I 269 CGGCCG C^GGCCG EcoRI 1748 GAATTC G^AATTC EcoRV 2282 GATATC GAT^ATC FbaI 3097 TGATCA T^GATCA HincII 2729 GTCAAC GT[CT]^[GA]AC HindII 2729 GTCAAC GT[CT]^[GA]AC KpnI 6032 GGTACC GGTAC^C MvnI 1048 CGCG CG^CG NruI 1047 TCGCGA TCG^CGA PinAI 1112 ACCGGT A^CCGGT PshAI 4390 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 736 GAAGAGC GCTCTTC(1/4) SbfI 2116 CCTGCAGG CCTGCA^GG ScaI 5134 AGTACT AGT^ACT SphI 2986 GCATGC GCATG^C Sse8387I 2116 CCTGCAGG CCTGCA^GG StuI 860 AGGCCT AGG^CCT XbaI 1925 TCTAGA T^CTAGA XmnI 409 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccI 1703 GTCTAC GT^[AC][GT]AC 4653 GTATAC AclI 4283 AACGTT AA^CGTT 5482 AACGTT AcsI 413 AAATTC [GA]^AATT[CT] 1748 GAATTC AflIII 921 ACATGT A^C[GA][CT]GT 5078 ACATGT AhdI 958 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 6336 GACAAGATGTC Alw44I 5104 GTGCAC G^TGCAC 5123 GTGCAC ApaLI 5104 GTGCAC G^TGCAC 5123 GTGCAC ApoI 413 AAATTC [GA]^AATT[CT] 1748 GAATTC AspEI 958 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 6336 GACAAGATGTC BalI 163 TGGCCA TGG^CCA 4647 TGGCCA BamHI 1886 GGATCC G^GATCC 5411 GGATCC BbsI 4927 GAAGAC GAAGAC(2/6) 6198 GTCTTC BciVI 5168 GGATAC GTATCC(6/5) 3626 GTATCC BlpI 332 GCTCAGC GC^T[ACGT]AGC 2339 GCTAAGC Bpu1102I 332 GCTCAGC GC^T[ACGT]AGC 2339 GCTAAGC BpuAI 4927 GAAGAC GAAGAC(2/6) 6198 GTCTTC BsiEI 269 CGGCCG CG[GA][CT]^CG 4338 CGGTCG BspLU11I 921 ACATGT A^CATGT 5078 ACATGT BsrBI 2243 CCGCTC CCGCTC(-3/-3) 5715 GAGCGG BsrDI 5632 CATTGC GCAATG(2/0) 6181 GCAATG BsrFI 1112 ACCGGT [GA]^CCGG[CT] 1125 ACCGGC BstXI 3615 CCACTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4951 CCAGTAGGATGG CelII 332 GCTCAGC GC^T[ACGT]AGC 2339 GCTAAGC Cfr10I 1112 ACCGGT [GA]^CCGG[CT] 1125 ACCGGC DraI 6256 TTTAAA TTT^AAA 6328 TTTAAA Eam1105I 958 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 6336 GACAAGATGTC EcoT22I 2001 ATGCAT ATGCA^T 5190 ATGCAT HindIII 1209 AAGCTT A^AGCTT 6014 AAGCTT MfeI 1989 CAATTG C^AATTG 4305 CAATTG MluNI 163 TGGCCA TGG^CCA 4647 TGGCCA MscI 163 TGGCCA TGG^CCA 4647 TGGCCA MunI 1989 CAATTG C^AATTG 4305 CAATTG NcoI 5096 CCATGG C^CATGG 6079 CCATGG NsiI 2001 ATGCAT ATGCA^T 5190 ATGCAT Psp1406I 4283 AACGTT AA^CGTT 5482 AACGTT ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI AocI AspHI AspI AvaII BanI BanII BbvCI BbvI BcnI BfaI BmyI BpmI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII