##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AccIII AflII Aor51HI AscI AseI
AsnI AviII BbeI BbrPI BcgI
BfrI BglI BglII BsaAI BsaBI
BseAI BsiWI BsmBI BspDI BspEI
BspHI BssHII BstBI BstEII BstPI
ClaI CpoI DraIII DrdI Eco47III
EcoO65I FseI FspI HaeII HpaI
KasI KspI MamI MluI MroI
NaeI NarI NdeI NgoMIV NheI
NotI NspV PacI PaeR7I PmaCI
PmeI PmlI PshBI PvuI RcaI
RsrII SacII SalI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SspI SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 1248 GACGTC GACGT^C
Acc65I 6032 GGTACC G^GTACC
AccII 1048 CGCG CG^CG
AgeI 1112 ACCGGT A^CCGGT
ApaI 4486 GGGCCC GGGCC^C
Asp700I 409 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 6032 GGTACC G^GTACC
AvaI 2178 CTCGGG C^[CT]CG[GA]G
AvrII 6028 CCTAGG C^CTAGG
BclI 3097 TGATCA T^GATCA
BlnI 6028 CCTAGG C^CTAGG
BsmI 191 GCATTC GAATGC(1/-1)
BsoBI 2178 CTCGGG C^[CT]CG[GA]G
BssSI 2890 CACGAG CACGAG(-5/-1)
Bst1107I 4653 GTATAC GTA^TAC
BstAPI 656 GCAGTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1048 CGCG CG^CG
BstZ17I 4653 GTATAC GTA^TAC
EagI 269 CGGCCG C^GGCCG
EclXI 269 CGGCCG C^GGCCG
Eco52I 269 CGGCCG C^GGCCG
EcoRI 1748 GAATTC G^AATTC
EcoRV 2282 GATATC GAT^ATC
FbaI 3097 TGATCA T^GATCA
HincII 2729 GTCAAC GT[CT]^[GA]AC
HindII 2729 GTCAAC GT[CT]^[GA]AC
KpnI 6032 GGTACC GGTAC^C
MvnI 1048 CGCG CG^CG
NruI 1047 TCGCGA TCG^CGA
PinAI 1112 ACCGGT A^CCGGT
PshAI 4390 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 736 GAAGAGC GCTCTTC(1/4)
SbfI 2116 CCTGCAGG CCTGCA^GG
ScaI 5134 AGTACT AGT^ACT
SphI 2986 GCATGC GCATG^C
Sse8387I 2116 CCTGCAGG CCTGCA^GG
StuI 860 AGGCCT AGG^CCT
XbaI 1925 TCTAGA T^CTAGA
XmnI 409 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 1703 GTCTAC GT^[AC][GT]AC
4653 GTATAC
AclI 4283 AACGTT AA^CGTT
5482 AACGTT
AcsI 413 AAATTC [GA]^AATT[CT]
1748 GAATTC
AflIII 921 ACATGT A^C[GA][CT]GT
5078 ACATGT
AhdI 958 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6336 GACAAGATGTC
Alw44I 5104 GTGCAC G^TGCAC
5123 GTGCAC
ApaLI 5104 GTGCAC G^TGCAC
5123 GTGCAC
ApoI 413 AAATTC [GA]^AATT[CT]
1748 GAATTC
AspEI 958 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6336 GACAAGATGTC
BalI 163 TGGCCA TGG^CCA
4647 TGGCCA
BamHI 1886 GGATCC G^GATCC
5411 GGATCC
BbsI 4927 GAAGAC GAAGAC(2/6)
6198 GTCTTC
BciVI 5168 GGATAC GTATCC(6/5)
3626 GTATCC
BlpI 332 GCTCAGC GC^T[ACGT]AGC
2339 GCTAAGC
Bpu1102I 332 GCTCAGC GC^T[ACGT]AGC
2339 GCTAAGC
BpuAI 4927 GAAGAC GAAGAC(2/6)
6198 GTCTTC
BsiEI 269 CGGCCG CG[GA][CT]^CG
4338 CGGTCG
BspLU11I 921 ACATGT A^CATGT
5078 ACATGT
BsrBI 2243 CCGCTC CCGCTC(-3/-3)
5715 GAGCGG
BsrDI 5632 CATTGC GCAATG(2/0)
6181 GCAATG
BsrFI 1112 ACCGGT [GA]^CCGG[CT]
1125 ACCGGC
BstXI 3615 CCACTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4951 CCAGTAGGATGG
CelII 332 GCTCAGC GC^T[ACGT]AGC
2339 GCTAAGC
Cfr10I 1112 ACCGGT [GA]^CCGG[CT]
1125 ACCGGC
DraI 6256 TTTAAA TTT^AAA
6328 TTTAAA
Eam1105I 958 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6336 GACAAGATGTC
EcoT22I 2001 ATGCAT ATGCA^T
5190 ATGCAT
HindIII 1209 AAGCTT A^AGCTT
6014 AAGCTT
MfeI 1989 CAATTG C^AATTG
4305 CAATTG
MluNI 163 TGGCCA TGG^CCA
4647 TGGCCA
MscI 163 TGGCCA TGG^CCA
4647 TGGCCA
MunI 1989 CAATTG C^AATTG
4305 CAATTG
NcoI 5096 CCATGG C^CATGG
6079 CCATGG
NsiI 2001 ATGCAT ATGCA^T
5190 ATGCAT
Psp1406I 4283 AACGTT AA^CGTT
5482 AACGTT
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI AspHI AspI AvaII
BanI BanII BbvCI BbvI BcnI
BfaI BmyI BpmI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspMI BsrGI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII