##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AccIII AflIII AgeI
AscI Asp718I AviII BamHI BbeI
BcgI BglII BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrFI
BsrGI BssHII BstEII BstPI Cfr10I
ClaI CpoI EagI EclXI Eco52I
EcoNI EcoO65I EcoRV FseI FspI
KasI KpnI KspI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NotI NruI PacI PaeR7I PinAI
PmeI PvuI RsrII SacII SalI
SbfI SfoI SgrAI SnaBI SphI
Sse8387I SspBI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccII 246 CGCG CG^CG
AclI 2457 AACGTT AA^CGTT
AcyI 26 GGCGTC G[GA]^CG[CT]C
AflII 1903 CTTAAG C^TTAAG
AhdI 2010 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 2760 ATTAAT AT^TAAT
AsnI 2760 ATTAAT AT^TAAT
AspEI 2010 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 5397 CCTAGG C^CTAGG
BanI 3410 GGTGCC G^G[CT][GA]CC
BbrPI 1640 CACGTG CAC^GTG
BbvCI 5225 GCTGAGG CCTCAGC(-5/-2)
BciVI 3181 GGATAC GTATCC(6/5)
BfrI 1903 CTTAAG C^TTAAG
BglI 163 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 5397 CCTAGG C^CTAGG
BsaHI 26 GGCGTC G[GA]^CG[CT]C
BsaI 3329 GAGACC GGTCTC(1/5)
BsaWI 2014 TCCGGT [AT]^CCGG[AT]
BsmBI 1173 GAGACG CGTCTC(1/5)
BspHI 2851 TCATGA T^CATGA
BsrBI 312 GAGCGG CCGCTC(-3/-3)
BsrDI 4406 CATTGC GCAATG(2/0)
BstUI 246 CGCG CG^CG
BstYI 5049 AGATCC [GA]^GATC[CT]
DraIII 1115 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4994 GACAGTAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2010 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2743 GAATTC G^AATTC
EcoT22I 5148 ATGCAT ATGCA^T
Hin1I 26 GGCGTC G[GA]^CG[CT]C
HindIII 1906 AAGCTT A^AGCTT
HpaI 2454 GTTAAC GTT^AAC
MflI 5049 AGATCC [GA]^GATC[CT]
MvnI 246 CGCG CG^CG
NheI 1584 GCTAGC G^CTAGC
NsiI 5148 ATGCAT ATGCA^T
PflMI 498 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1640 CACGTG CAC^GTG
PmlI 1640 CACGTG CAC^GTG
PpuMI 320 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 2760 ATTAAT AT^TAAT
Psp1406I 2457 AACGTT AA^CGTT
RcaI 2851 TCATGA T^CATGA
SacI 971 GAGCTC GAGCT^C
SexAI 2837 ACCTGGT A^CC[AT]GGT
SfiI 162 GGCCATCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 1388 AGGCCT AGG^CCT
Van91I 498 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 900 TCTAGA T^CTAGA
XcmI 4038 CCAGCCCTGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 5049 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Aor51HI 2205 AGCGCT AGC^GCT
2907 AGCGCT
ApaI 58 GGGCCC GGGCC^C
1600 GGGCCC
Asp700I 382 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
826 GAAGAAGTTC
AspI 2404 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2509 GACATTGTC
BlpI 2098 GCTCAGC GC^T[ACGT]AGC
4006 GCTCAGC
Bpu1102I 2098 GCTCAGC GC^T[ACGT]AGC
4006 GCTCAGC
BsaAI 1640 CACGTG [CT]AC^GT[GA]
4477 CACGTA
BsaBI 3182 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4342 GATTCTCATC
BsmI 2287 GAATGC GAATGC(1/-1)
2223 GCATTC
BssSI 271 CTCGTG CACGAG(-5/-1)
1075 CTCGTG
Bst1107I 1182 GTATAC GTA^TAC
2704 GTATAC
BstZ17I 1182 GTATAC GTA^TAC
2704 GTATAC
CelII 2098 GCTCAGC GC^T[ACGT]AGC
4006 GCTCAGC
DsaI 3965 CCATGG C^C[GA][CT]GG
5592 CCATGG
Eco47III 2205 AGCGCT AGC^GCT
2907 AGCGCT
FauI 1568 CCCGC CCCGC(4/6)
521 GCGGG
HgaI 2181 GACGC GACGC(5/10)
27 GCGTC
MamI 3182 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4342 GATTCTCATC
NcoI 3965 CCATGG C^CATGG
5592 CCATGG
NdeI 440 CATATG CA^TATG
5575 CATATG
PflFI 2404 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2509 GACATTGTC
PshAI 96 GACGGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
726 GACACAAGTC
PstI 589 CTGCAG CTGCA^G
1687 CTGCAG
SapI 309 GAAGAGC GCTCTTC(1/4)
973 GCTCTTC
ScaI 198 AGTACT AGT^ACT
2574 AGTACT
SmaI 1059 CCCGGG CCC^GGG
1596 CCCGGG
SspI 4885 AATATT AAT^ATT
5500 AATATT
SwaI 2436 ATTTAAAT ATTT^AAAT
4713 ATTTAAAT
Tth111I 2404 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2509 GACATTGTC
XmaCI 1059 CCCGGG C^CCGGG
1596 CCCGGG
XmaI 1059 CCCGGG C^CCGGG
1596 CCCGGG
XmnI 382 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
826 GAAGAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
Alw44I AlwI AlwNI AocI ApaLI
ApoI AspHI AvaI AvaII BalI
BanII BbsI BbvI BclI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstBI BstF5I
BstNI BstXI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FbaI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
NspV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfuI
SmlI SpeI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I