################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI AlwNI AscI Asp718I AspI AviII AvrII BbvCI BglI BglII BlnI BsaWI BseAI BsiWI BsmBI BspDI BspEI BssHII Bst1107I BstZ17I ClaI CpoI DrdI EagI EclXI Eco52I EcoRV FseI FspI HincII HindII HpaI KpnI MfeI MluI MroI MunI NaeI NcoI NgoMIV NotI PacI PflFI PinAI PmeI PshAI Psp1406I PvuI RsrII SacI SalI SbfI ScaI SexAI SfiI SgrAI SpeI Sse8387I SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 2188 GTCTAC GT^[AC][GT]AC AcyI 57 GGCGCC G[GA]^CG[CT]C AflII 1261 CTTAAG C^TTAAG Alw44I 948 GTGCAC G^TGCAC ApaI 1872 GGGCCC GGGCC^C ApaLI 948 GTGCAC G^TGCAC BalI 570 TGGCCA TGG^CCA BbeI 57 GGCGCC GGCGC^C BbrPI 3833 CACGTG CAC^GTG BcgI 857 GCACCCAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3615 GGATAC GTATCC(6/5) BclI 4845 TGATCA T^GATCA BfrI 1261 CTTAAG C^TTAAG BlpI 2536 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2536 GCTGAGC GC^T[ACGT]AGC BsaBI 3616 GATACGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 57 GGCGCC G[GA]^CG[CT]C BsgI 3241 CTGCAC GTGCAG(16/14) BsiEI 747 CGACCG CG[GA][CT]^CG BsrFI 421 GCCGGT [GA]^CCGG[CT] BstBI 2199 TTCGAA TT^CGAA BstXI 2132 CCATTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 2536 GCTGAGC GC^T[ACGT]AGC Cfr10I 421 GCCGGT [GA]^CCGG[CT] DsaI 750 CCGCGG C^C[GA][CT]GG FbaI 4845 TGATCA T^GATCA Hin1I 57 GGCGCC G[GA]^CG[CT]C KasI 57 GGCGCC G^GCGCC KspI 750 CCGCGG CCGC^GG MamI 3616 GATACGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 570 TGGCCA TGG^CCA MscI 570 TGGCCA TGG^CCA NarI 57 GGCGCC GG^CGCC NheI 286 GCTAGC G^CTAGC NruI 8 TCGCGA TCG^CGA NspV 2199 TTCGAA TT^CGAA PflMI 4956 CCAATTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 3833 CACGTG CAC^GTG PmlI 3833 CACGTG CAC^GTG PvuII 384 CAGCTG CAG^CTG SacII 750 CCGCGG CCGC^GG SapI 4874 GAAGAGC GCTCTTC(1/4) SfoI 57 GGCGCC GGC^GCC SfuI 2199 TTCGAA TT^CGAA Van91I 4956 CCAATTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 545 CCAAAAGAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AhdI 2059 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3010 GACTACATGTC Aor51HI 761 AGCGCT AGC^GCT 3284 AGCGCT Asp700I 1047 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3198 GAAAGCGTTC AspEI 2059 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3010 GACTACATGTC BamHI 1575 GGATCC G^GATCC 3621 GGATCC BsaI 3171 GAGACC GGTCTC(1/5) 2288 GGTCTC BspHI 1947 TCATGA T^CATGA 4244 TCATGA BspLU11I 2063 ACATGT A^CATGT 3014 ACATGT BsrBI 88 CCGCTC CCGCTC(-3/-3) 3358 GAGCGG BstAPI 906 GCACCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2080 GCATACTATGC BstYI 1575 GGATCC [GA]^GATC[CT] 3621 GGATCC DraIII 574 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG 951 CACGGAGTG Eam1105I 2059 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3010 GACTACATGTC Eco47III 761 AGCGCT AGC^GCT 3284 AGCGCT EcoNI 2145 CCTTCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4420 CCTTTGTAAGG EcoT22I 3095 ATGCAT ATGCA^T 4340 ATGCAT MflI 1575 GGATCC [GA]^GATC[CT] 3621 GGATCC MspA1I 384 CAGCTG C[AC]G^C[GT]G 750 CCGCGG NsiI 3095 ATGCAT ATGCA^T 4340 ATGCAT PpuMI 1066 GGGTCCT [GA]G^G[AT]CC[CT] 2227 AGGACCT PstI 2530 CTGCAG CTGCA^G 3436 CTGCAG RcaI 1947 TCATGA T^CATGA 4244 TCATGA SmaI 1869 CCCGGG CCC^GGG 2851 CCCGGG SnaBI 4059 TACGTA TAC^GTA 4864 TACGTA SphI 290 GCATGC GCATG^C 3485 GCATGC SspI 1618 AATATT AAT^ATT 2401 AATATT XhoII 1575 GGATCC [GA]^GATC[CT] 3621 GGATCC XmaCI 1869 CCCGGG C^CCGGG 2851 CCCGGG XmaI 1869 CCCGGG C^CCGGG 2851 CCCGGG XmnI 1047 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3198 GAAAGCGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AocI ApoI AseI AsnI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BssSI BstEII BstF5I BstNI BstPI BstUI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI Eco81I EcoO109I EcoO65I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspI MvaI MvnI MwoI NciI NdeI NdeII NlaIII NlaIV NspI PaeR7I PleI PshBI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoI