##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AlwNI AscI Asp718I AspI AviII
AvrII BbvCI BglI BglII BlnI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BssHII Bst1107I BstZ17I ClaI
CpoI DrdI EagI EclXI Eco52I
EcoRV FseI FspI HincII HindII
HpaI KpnI MfeI MluI MroI
MunI NaeI NcoI NgoMIV NotI
PacI PflFI PinAI PmeI PshAI
Psp1406I PvuI RsrII SacI SalI
SbfI ScaI SexAI SfiI SgrAI
SpeI Sse8387I SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 2188 GTCTAC GT^[AC][GT]AC
AcyI 57 GGCGCC G[GA]^CG[CT]C
AflII 1261 CTTAAG C^TTAAG
Alw44I 948 GTGCAC G^TGCAC
ApaI 1872 GGGCCC GGGCC^C
ApaLI 948 GTGCAC G^TGCAC
BalI 570 TGGCCA TGG^CCA
BbeI 57 GGCGCC GGCGC^C
BbrPI 3833 CACGTG CAC^GTG
BcgI 857 GCACCCAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3615 GGATAC GTATCC(6/5)
BclI 4845 TGATCA T^GATCA
BfrI 1261 CTTAAG C^TTAAG
BlpI 2536 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2536 GCTGAGC GC^T[ACGT]AGC
BsaBI 3616 GATACGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 57 GGCGCC G[GA]^CG[CT]C
BsgI 3241 CTGCAC GTGCAG(16/14)
BsiEI 747 CGACCG CG[GA][CT]^CG
BsrFI 421 GCCGGT [GA]^CCGG[CT]
BstBI 2199 TTCGAA TT^CGAA
BstXI 2132 CCATTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2536 GCTGAGC GC^T[ACGT]AGC
Cfr10I 421 GCCGGT [GA]^CCGG[CT]
DsaI 750 CCGCGG C^C[GA][CT]GG
FbaI 4845 TGATCA T^GATCA
Hin1I 57 GGCGCC G[GA]^CG[CT]C
KasI 57 GGCGCC G^GCGCC
KspI 750 CCGCGG CCGC^GG
MamI 3616 GATACGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 570 TGGCCA TGG^CCA
MscI 570 TGGCCA TGG^CCA
NarI 57 GGCGCC GG^CGCC
NheI 286 GCTAGC G^CTAGC
NruI 8 TCGCGA TCG^CGA
NspV 2199 TTCGAA TT^CGAA
PflMI 4956 CCAATTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3833 CACGTG CAC^GTG
PmlI 3833 CACGTG CAC^GTG
PvuII 384 CAGCTG CAG^CTG
SacII 750 CCGCGG CCGC^GG
SapI 4874 GAAGAGC GCTCTTC(1/4)
SfoI 57 GGCGCC GGC^GCC
SfuI 2199 TTCGAA TT^CGAA
Van91I 4956 CCAATTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 545 CCAAAAGAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AhdI 2059 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3010 GACTACATGTC
Aor51HI 761 AGCGCT AGC^GCT
3284 AGCGCT
Asp700I 1047 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3198 GAAAGCGTTC
AspEI 2059 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3010 GACTACATGTC
BamHI 1575 GGATCC G^GATCC
3621 GGATCC
BsaI 3171 GAGACC GGTCTC(1/5)
2288 GGTCTC
BspHI 1947 TCATGA T^CATGA
4244 TCATGA
BspLU11I 2063 ACATGT A^CATGT
3014 ACATGT
BsrBI 88 CCGCTC CCGCTC(-3/-3)
3358 GAGCGG
BstAPI 906 GCACCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2080 GCATACTATGC
BstYI 1575 GGATCC [GA]^GATC[CT]
3621 GGATCC
DraIII 574 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
951 CACGGAGTG
Eam1105I 2059 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3010 GACTACATGTC
Eco47III 761 AGCGCT AGC^GCT
3284 AGCGCT
EcoNI 2145 CCTTCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4420 CCTTTGTAAGG
EcoT22I 3095 ATGCAT ATGCA^T
4340 ATGCAT
MflI 1575 GGATCC [GA]^GATC[CT]
3621 GGATCC
MspA1I 384 CAGCTG C[AC]G^C[GT]G
750 CCGCGG
NsiI 3095 ATGCAT ATGCA^T
4340 ATGCAT
PpuMI 1066 GGGTCCT [GA]G^G[AT]CC[CT]
2227 AGGACCT
PstI 2530 CTGCAG CTGCA^G
3436 CTGCAG
RcaI 1947 TCATGA T^CATGA
4244 TCATGA
SmaI 1869 CCCGGG CCC^GGG
2851 CCCGGG
SnaBI 4059 TACGTA TAC^GTA
4864 TACGTA
SphI 290 GCATGC GCATG^C
3485 GCATGC
SspI 1618 AATATT AAT^ATT
2401 AATATT
XhoII 1575 GGATCC [GA]^GATC[CT]
3621 GGATCC
XmaCI 1869 CCCGGG C^CCGGG
2851 CCCGGG
XmaI 1869 CCCGGG C^CCGGG
2851 CCCGGG
XmnI 1047 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3198 GAAAGCGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AocI ApoI AseI
AsnI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspMI BsrDI BsrGI BsrI BssKI
BssSI BstEII BstF5I BstNI BstPI
BstUI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI Eco81I EcoO109I EcoO65I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MvnI MwoI NciI
NdeI NdeII NlaIII NlaIV NspI
PaeR7I PleI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoI