##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
Acc65I AclI AflII AgeI ApaI
AscI Asp718I AviII BalI BamHI
BbeI BfrI BlpI Bpu1102I BsaBI
BsiEI BsiWI BsmI BspDI BsrFI
BssHII Bst1107I BstBI BstZ17I CelII
Cfr10I ClaI CpoI DrdI EagI
EclXI Eco52I EcoRV FseI FspI
KasI KpnI KspI MamI MfeI
MluI MluNI MscI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshAI Psp1406I PvuI
RsrII SacII SalI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 2814 GACGTC GACGT^C
AccII 6070 CGCG CG^CG
AccIII 3525 TCCGGA T^CCGGA
AhdI 2138 GACAAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 242 GTGCAC G^TGCAC
ApaLI 242 GTGCAC G^TGCAC
AspEI 2138 GACAAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1433 CACGTG CAC^GTG
BbvCI 831 CCTCAGC CCTCAGC(-5/-2)
BcgI 1720 CGAAAAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 6476 TGATCA T^GATCA
BglI 5872 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 3525 TCCGGA [AT]^CCGG[AT]
BseAI 3525 TCCGGA T^CCGGA
BsmBI 1869 CGTCTC CGTCTC(1/5)
BspEI 3525 TCCGGA T^CCGGA
BspHI 3869 TCATGA T^CATGA
BsrBI 870 GAGCGG CCGCTC(-3/-3)
BstAPI 4755 GCAGAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 6070 CGCG CG^CG
DraII 5144 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 4669 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2138 GACAAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1510 CCTCAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 5144 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 4470 GAATTC G^AATTC
FbaI 6476 TGATCA T^GATCA
HpaI 1403 GTTAAC GTT^AAC
MroI 3525 TCCGGA T^CCGGA
MvnI 6070 CGCG CG^CG
PflMI 3052 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1433 CACGTG CAC^GTG
PmlI 1433 CACGTG CAC^GTG
PpuMI 5144 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 3869 TCATGA T^CATGA
SbfI 2102 CCTGCAGG CCTGCA^GG
ScaI 4153 AGTACT AGT^ACT
SpeI 2722 ACTAGT A^CTAGT
SphI 156 GCATGC GCATG^C
Sse8387I 2102 CCTGCAGG CCTGCA^GG
StuI 101 AGGCCT AGG^CCT
SwaI 4263 ATTTAAAT ATTT^AAAT
Van91I 3052 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 655 GTCTAC GT^[AC][GT]AC
2135 GTAGAC
AcyI 2814 GACGTC G[GA]^CG[CT]C
3472 GACGCC
AocI 3216 CCTCAGG CC^T[ACGT]AGG
4828 CCTTAGG
Aor51HI 271 AGCGCT AGC^GCT
4132 AGCGCT
AseI 200 ATTAAT AT^TAAT
6620 ATTAAT
AsnI 200 ATTAAT AT^TAAT
6620 ATTAAT
AspI 2855 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5840 GACAGGGTC
AvrII 4548 CCTAGG C^CTAGG
6418 CCTAGG
BciVI 294 GGATAC GTATCC(6/5)
6672 GTATCC
BlnI 4548 CCTAGG C^CTAGG
6418 CCTAGG
BsaAI 1433 CACGTG [CT]AC^GT[GA]
5884 TACGTG
BsaHI 2814 GACGTC G[GA]^CG[CT]C
3472 GACGCC
BssSI 1296 CACGAG CACGAG(-5/-1)
1110 CTCGTG
BstEII 3230 GGTAACC G^GT[ACGT]ACC
4823 GGTAACC
BstPI 3230 GGTAACC G^GT[ACGT]ACC
4823 GGTAACC
Bsu36I 3216 CCTCAGG CC^T[ACGT]AGG
4828 CCTTAGG
EaeI 1603 TGGCCG [CT]^GGCC[GA]
4588 TGGCCG
Eco47III 271 AGCGCT AGC^GCT
4132 AGCGCT
Eco81I 3216 CCTCAGG CC^T[ACGT]AGG
4828 CCTTAGG
EcoO65I 3230 GGTAACC G^GT[ACGT]ACC
4823 GGTAACC
EcoT22I 154 ATGCAT ATGCA^T
2836 ATGCAT
Hin1I 2814 GACGTC G[GA]^CG[CT]C
3472 GACGCC
NsiI 154 ATGCAT ATGCA^T
2836 ATGCAT
PflFI 2855 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5840 GACAGGGTC
PshBI 200 ATTAAT AT^TAAT
6620 ATTAAT
SapI 821 GAAGAGC GCTCTTC(1/4)
1365 GCTCTTC
Tth111I 2855 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5840 GACAGGGTC
XbaI 6693 TCTAGA T^CTAGA
6732 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspLU11I
BspMI BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DsaI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI