################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI AhdI AscI AseI AsnI Asp718I AspEI AviII BbeI BclI BsaBI BsaHI BseAI BsiEI BsiWI BspDI BspEI BsrBI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV FbaI FseI FspI Hin1I HpaI KasI KpnI KspI MamI MluI MroI NarI NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AflII 5421 CTTAAG C^TTAAG Alw44I 1044 GTGCAC G^TGCAC Aor51HI 4574 AGCGCT AGC^GCT ApaLI 1044 GTGCAC G^TGCAC AspI 2226 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1315 GGATCC G^GATCC BbrPI 1879 CACGTG CAC^GTG BfrI 5421 CTTAAG C^TTAAG BglI 952 GCCATTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1188 AGATCT A^GATCT BlpI 3934 GCTGAGC GC^T[ACGT]AGC Bpu1102I 3934 GCTGAGC GC^T[ACGT]AGC BsaAI 1879 CACGTG [CT]AC^GT[GA] BsaWI 4058 ACCGGA [AT]^CCGG[AT] BsmBI 261 CGTCTC CGTCTC(1/5) Bsp1407I 2389 TGTACA T^GTACA BspLU11I 1937 ACATGT A^CATGT BsrFI 2308 GCCGGC [GA]^CCGG[CT] BsrGI 2389 TGTACA T^GTACA BssSI 2328 CACGAG CACGAG(-5/-1) CelII 3934 GCTGAGC GC^T[ACGT]AGC Cfr10I 2308 GCCGGC [GA]^CCGG[CT] Eco47III 4574 AGCGCT AGC^GCT EcoT22I 3244 ATGCAT ATGCA^T HgaI 1294 GCGTC GACGC(5/10) MfeI 1701 CAATTG C^AATTG MunI 1701 CAATTG C^AATTG NaeI 2308 GCCGGC GCC^GGC NdeI 1342 CATATG CA^TATG NgoMIV 2308 GCCGGC G^CCGGC NsiI 3244 ATGCAT ATGCA^T PflFI 2226 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1879 CACGTG CAC^GTG PmlI 1879 CACGTG CAC^GTG SalI 5231 GTCGAC G^TCGAC SphI 4643 GCATGC GCATG^C SspBI 2389 TGTACA T^GTACA Tth111I 2226 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccII 40 CGCG CG^CG 836 CGCG AvaI 3530 CTCGGG C^[CT]CG[GA]G 4346 CTCGGG BalI 3407 TGGCCA TGG^CCA 3545 TGGCCA BcgI 425 GCATGGGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 761 GCACATCCTTCG BsoBI 3530 CTCGGG C^[CT]CG[GA]G 4346 CTCGGG BspHI 1369 TCATGA T^CATGA 1525 TCATGA BspMI 285 ACCTGC ACCTGC(4/8) 2454 ACCTGC BstUI 40 CGCG CG^CG 836 CGCG DraI 1802 TTTAAA TTT^AAA 1812 TTTAAA DrdI 459 GACCAGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1742 GACACACAGGTC EaeI 3407 TGGCCA [CT]^GGCC[GA] 3545 TGGCCA FauI 3089 CCCGC CCCGC(4/6) 41 GCGGG HindIII 2334 AAGCTT A^AGCTT 2360 AAGCTT MluNI 3407 TGGCCA TGG^CCA 3545 TGGCCA MscI 3407 TGGCCA TGG^CCA 3545 TGGCCA MvnI 40 CGCG CG^CG 836 CGCG RcaI 1369 TCATGA T^CATGA 1525 TCATGA SexAI 2227 ACCAGGT A^CC[AT]GGT 4926 ACCTGGT StuI 1493 AGGCCT AGG^CCT 3449 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApaI ApoI Asp700I AspHI AvaII AvrII BanI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BlnI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrDI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PshAI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI