##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AviII BbeI BclI
BsaBI BsaHI BseAI BsiEI BsiWI
BspDI BspEI BsrBI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRI EcoRV FbaI FseI
FspI Hin1I HpaI KasI KpnI
KspI MamI MluI MroI NarI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AflII 5421 CTTAAG C^TTAAG
Alw44I 1044 GTGCAC G^TGCAC
Aor51HI 4574 AGCGCT AGC^GCT
ApaLI 1044 GTGCAC G^TGCAC
AspI 2226 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1315 GGATCC G^GATCC
BbrPI 1879 CACGTG CAC^GTG
BfrI 5421 CTTAAG C^TTAAG
BglI 952 GCCATTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1188 AGATCT A^GATCT
BlpI 3934 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3934 GCTGAGC GC^T[ACGT]AGC
BsaAI 1879 CACGTG [CT]AC^GT[GA]
BsaWI 4058 ACCGGA [AT]^CCGG[AT]
BsmBI 261 CGTCTC CGTCTC(1/5)
Bsp1407I 2389 TGTACA T^GTACA
BspLU11I 1937 ACATGT A^CATGT
BsrFI 2308 GCCGGC [GA]^CCGG[CT]
BsrGI 2389 TGTACA T^GTACA
BssSI 2328 CACGAG CACGAG(-5/-1)
CelII 3934 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2308 GCCGGC [GA]^CCGG[CT]
Eco47III 4574 AGCGCT AGC^GCT
EcoT22I 3244 ATGCAT ATGCA^T
HgaI 1294 GCGTC GACGC(5/10)
MfeI 1701 CAATTG C^AATTG
MunI 1701 CAATTG C^AATTG
NaeI 2308 GCCGGC GCC^GGC
NdeI 1342 CATATG CA^TATG
NgoMIV 2308 GCCGGC G^CCGGC
NsiI 3244 ATGCAT ATGCA^T
PflFI 2226 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1879 CACGTG CAC^GTG
PmlI 1879 CACGTG CAC^GTG
SalI 5231 GTCGAC G^TCGAC
SphI 4643 GCATGC GCATG^C
SspBI 2389 TGTACA T^GTACA
Tth111I 2226 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 40 CGCG CG^CG
836 CGCG
AvaI 3530 CTCGGG C^[CT]CG[GA]G
4346 CTCGGG
BalI 3407 TGGCCA TGG^CCA
3545 TGGCCA
BcgI 425 GCATGGGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
761 GCACATCCTTCG
BsoBI 3530 CTCGGG C^[CT]CG[GA]G
4346 CTCGGG
BspHI 1369 TCATGA T^CATGA
1525 TCATGA
BspMI 285 ACCTGC ACCTGC(4/8)
2454 ACCTGC
BstUI 40 CGCG CG^CG
836 CGCG
DraI 1802 TTTAAA TTT^AAA
1812 TTTAAA
DrdI 459 GACCAGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1742 GACACACAGGTC
EaeI 3407 TGGCCA [CT]^GGCC[GA]
3545 TGGCCA
FauI 3089 CCCGC CCCGC(4/6)
41 GCGGG
HindIII 2334 AAGCTT A^AGCTT
2360 AAGCTT
MluNI 3407 TGGCCA TGG^CCA
3545 TGGCCA
MscI 3407 TGGCCA TGG^CCA
3545 TGGCCA
MvnI 40 CGCG CG^CG
836 CGCG
RcaI 1369 TCATGA T^CATGA
1525 TCATGA
SexAI 2227 ACCAGGT A^CC[AT]GGT
4926 ACCTGGT
StuI 1493 AGGCCT AGG^CCT
3449 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
ApoI Asp700I AspHI AvaII AvrII
BanI BanII BbsI BbvCI BbvI
BciVI BcnI BfaI BlnI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PshAI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI