##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AclI AflIII AgeI Aor51HI
AscI Asp718I AspI AvrII BamHI
BbeI BbrPI BlnI BsaBI BsiWI
Bsp1407I BspDI BspLU11I BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI Eco47III EcoO65I EcoRV
FseI HpaI KasI KpnI MamI
MluI NarI NdeI NheI NotI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 1924 GACGTC GACGT^C
AccI 3714 GTAGAC GT^[AC][GT]AC
AccIII 690 TCCGGA T^CCGGA
AflII 4683 CTTAAG C^TTAAG
AhdI 1527 GACTCATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2364 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 3858 GGGCCC GGGCC^C
AseI 3533 ATTAAT AT^TAAT
AsnI 3533 ATTAAT AT^TAAT
Asp700I 2229 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1527 GACTCATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BclI 3112 TGATCA T^GATCA
BfrI 4683 CTTAAG C^TTAAG
BseAI 690 TCCGGA T^CCGGA
BsmBI 1912 GAGACG CGTCTC(1/5)
BspEI 690 TCCGGA T^CCGGA
BspHI 1496 TCATGA T^CATGA
BsrDI 2263 GCAATG GCAATG(2/0)
BstAPI 404 GCAGTAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 1227 GACTGACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 52 CGGCCG C^GGCCG
Eam1105I 1527 GACTCATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 52 CGGCCG C^GGCCG
Eco52I 52 CGGCCG C^GGCCG
EcoNI 3329 CCTTCTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 3112 TGATCA T^GATCA
HaeII 730 AGCGCC [GA]GCGC^[CT]
HincII 3188 GTTGAC GT[CT]^[GA]AC
HindII 3188 GTTGAC GT[CT]^[GA]AC
KspI 77 CCGCGG CCGC^GG
MfeI 4034 CAATTG C^AATTG
MroI 690 TCCGGA T^CCGGA
MunI 4034 CAATTG C^AATTG
NaeI 50 GCCGGC GCC^GGC
NcoI 3719 CCATGG C^CATGG
NgoMIV 50 GCCGGC G^CCGGC
PaeR7I 320 CTCGAG C^TCGAG
PshBI 3533 ATTAAT AT^TAAT
RcaI 1496 TCATGA T^CATGA
SacII 77 CCGCGG CCGC^GG
SspI 151 AATATT AAT^ATT
StuI 977 AGGCCT AGG^CCT
XbaI 3986 TCTAGA T^CTAGA
XhoI 320 CTCGAG C^TCGAG
XmnI 2229 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Alw44I 159 GTGCAC G^TGCAC
925 GTGCAC
AocI 4261 CCTAAGG CC^T[ACGT]AGG
4702 CCTAAGG
ApaLI 159 GTGCAC G^TGCAC
925 GTGCAC
BbsI 718 GAAGAC GAAGAC(2/6)
3640 GAAGAC
BbvCI 960 CCTCAGC CCTCAGC(-5/-2)
1160 CCTCAGC
BcgI 546 CGAGATCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4329 GCAGAGGACTCG
BciVI 3274 GGATAC GTATCC(6/5)
3006 GTATCC
BpuAI 718 GAAGAC GAAGAC(2/6)
3640 GAAGAC
BsaAI 483 TACGTG [CT]AC^GT[GA]
2728 CACGTA
BsmI 4370 GAATGC GAATGC(1/-1)
4004 GCATTC
BsrBI 2392 CCGCTC CCGCTC(-3/-3)
1967 GAGCGG
BstXI 1353 CCAGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2505 CCACAAGATTGG
Bsu36I 4261 CCTAAGG CC^T[ACGT]AGG
4702 CCTAAGG
DraI 2602 TTTAAA TTT^AAA
4010 TTTAAA
DraIII 429 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2971 CACTCGGTG
Eco81I 4261 CCTAAGG CC^T[ACGT]AGG
4702 CCTAAGG
EcoRI 1791 GAATTC G^AATTC
4752 GAATTC
HindIII 2656 AAGCTT A^AGCTT
2679 AAGCTT
NruI 34 TCGCGA TCG^CGA
755 TCGCGA
PpuMI 437 GGGACCT [GA]G^G[AT]CC[CT]
4174 GGGACCT
PshAI 1920 GACCGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2038 GACAGTAGTC
SacI 1864 GAGCTC GAGCT^C
3084 GAGCTC
SmaI 98 CCCGGG CCC^GGG
134 CCCGGG
SphI 381 GCATGC GCATG^C
1464 GCATGC
XcmI 800 CCAAGGTGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1767 CCAGCTCCCAGCTGG
XmaCI 98 CCCGGG C^CCGGG
134 CCCGGG
XmaI 98 CCCGGG C^CCGGG
134 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AspHI AvaI
AvaII AviII BalI BanI BanII
BbvI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
FspI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII