################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AatII AccII AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI AspEI BbrPI BfrI BglII BsaAI BsiWI BspDI BsrBI BssHII BssSI BstUI ClaI CpoI Eam1105I Eco47III EcoRI EcoT22I FseI HpaI KspI MfeI MluI MunI MvnI NdeI NotI NruI NsiI PacI PinAI PmaCI PmeI PmlI PshAI PshBI RsrII SacII SalI SbfI SgrAI SmaI SnaBI SphI Sse8387I SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AclI 2180 AACGTT AA^CGTT Asp700I 2178 GAAACGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 4883 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1196 TGCGCA TGC^GCA AvrII 5244 CCTAGG C^CTAGG BalI 1866 TGGCCA TGG^CCA BbeI 4142 GGCGCC GGCGC^C BbvCI 2031 GCTGAGG CCTCAGC(-5/-2) BcgI 2618 GCAACGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3663 GGATAC GTATCC(6/5) BlnI 5244 CCTAGG C^CTAGG BsaBI 1647 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BstBI 1773 TTCGAA TT^CGAA BstEII 5196 GGTGACC G^GT[ACGT]ACC BstPI 5196 GGTGACC G^GT[ACGT]ACC DraIII 3811 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 3946 CGGCCG C^GGCCG EclXI 3946 CGGCCG C^GGCCG Eco52I 3946 CGGCCG C^GGCCG EcoNI 3535 CCTATGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 5196 GGTGACC G^GT[ACGT]ACC FspI 1196 TGCGCA TGC^GCA HincII 3148 GTCAAC GT[CT]^[GA]AC HindII 3148 GTCAAC GT[CT]^[GA]AC KasI 4142 GGCGCC G^GCGCC MamI 1647 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1866 TGGCCA TGG^CCA MscI 1866 TGGCCA TGG^CCA NaeI 3948 GCCGGC GCC^GGC NarI 4142 GGCGCC GG^CGCC NgoMIV 3948 GCCGGC G^CCGGC NheI 5110 GCTAGC G^CTAGC NspV 1773 TTCGAA TT^CGAA PflFI 4883 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 2180 AACGTT AA^CGTT PvuI 2168 CGATCG CGAT^CG SapI 4868 GCTCTTC GCTCTTC(1/4) SexAI 3812 ACCTGGT A^CC[AT]GGT SfiI 1745 GGCCTGTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 4142 GGCGCC GGC^GCC SfuI 1773 TTCGAA TT^CGAA SpeI 1233 ACTAGT A^CTAGT SspI 2746 AATATT AAT^ATT StuI 1744 AGGCCT AGG^CCT Tth111I 4883 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 2178 GAAACGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccIII 2222 TCCGGA T^CCGGA 4209 TCCGGA AflIII 1943 ACATGT A^C[GA][CT]GT 3842 ACATGT BamHI 2452 GGATCC G^GATCC 3639 GGATCC BclI 2712 TGATCA T^GATCA 3774 TGATCA BlpI 1487 GCTCAGC GC^T[ACGT]AGC 4966 GCTAAGC Bpu1102I 1487 GCTCAGC GC^T[ACGT]AGC 4966 GCTAAGC BsaWI 2222 TCCGGA [AT]^CCGG[AT] 4209 TCCGGA BseAI 2222 TCCGGA T^CCGGA 4209 TCCGGA BsiEI 2168 CGATCG CG[GA][CT]^CG 3946 CGGCCG BsmBI 845 CGTCTC CGTCTC(1/5) 1115 CGTCTC BsmI 2410 GAATGC GAATGC(1/-1) 3978 GAATGC BspEI 2222 TCCGGA T^CCGGA 4209 TCCGGA BspLU11I 1943 ACATGT A^CATGT 3842 ACATGT BstAPI 243 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4398 GCAGACCTTGC CelII 1487 GCTCAGC GC^T[ACGT]AGC 4966 GCTAAGC DrdI 1902 GACCCCGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3223 GACTACTCTGTC FbaI 2712 TGATCA T^GATCA 3774 TGATCA HindIII 825 AAGCTT A^AGCTT 1630 AAGCTT MroI 2222 TCCGGA T^CCGGA 4209 TCCGGA MspA1I 2270 CAGCTG C[AC]G^C[GT]G 4728 CAGCTG NcoI 4717 CCATGG C^CATGG 5495 CCATGG PaeR7I 1833 CTCGAG C^TCGAG 4037 CTCGAG PflMI 2136 CCAATACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3378 CCAGTGGGTGG PvuII 2270 CAGCTG CAG^CTG 4728 CAGCTG ScaI 4441 AGTACT AGT^ACT 5262 AGTACT TfiI 814 GAATC G^A[AT]TC 4374 GATTC Van91I 2136 CCAATACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3378 CCAGTGGGTGG XhoI 1833 CTCGAG C^TCGAG 4037 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### Acc65I AccI AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApaI ApoI Asp718I AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspHI BspMI BsrDI BsrFI BsrGI BsrI BssKI Bst1107I BstF5I BstNI BstXI BstYI BstZ17I Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoRV EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII