##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AccII AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AspEI BbrPI BfrI BglII
BsaAI BsiWI BspDI BsrBI BssHII
BssSI BstUI ClaI CpoI Eam1105I
Eco47III EcoRI EcoT22I FseI HpaI
KspI MfeI MluI MunI MvnI
NdeI NotI NruI NsiI PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI RsrII SacII SalI SbfI
SgrAI SmaI SnaBI SphI Sse8387I
SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AclI 2180 AACGTT AA^CGTT
Asp700I 2178 GAAACGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4883 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1196 TGCGCA TGC^GCA
AvrII 5244 CCTAGG C^CTAGG
BalI 1866 TGGCCA TGG^CCA
BbeI 4142 GGCGCC GGCGC^C
BbvCI 2031 GCTGAGG CCTCAGC(-5/-2)
BcgI 2618 GCAACGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3663 GGATAC GTATCC(6/5)
BlnI 5244 CCTAGG C^CTAGG
BsaBI 1647 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BstBI 1773 TTCGAA TT^CGAA
BstEII 5196 GGTGACC G^GT[ACGT]ACC
BstPI 5196 GGTGACC G^GT[ACGT]ACC
DraIII 3811 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3946 CGGCCG C^GGCCG
EclXI 3946 CGGCCG C^GGCCG
Eco52I 3946 CGGCCG C^GGCCG
EcoNI 3535 CCTATGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 5196 GGTGACC G^GT[ACGT]ACC
FspI 1196 TGCGCA TGC^GCA
HincII 3148 GTCAAC GT[CT]^[GA]AC
HindII 3148 GTCAAC GT[CT]^[GA]AC
KasI 4142 GGCGCC G^GCGCC
MamI 1647 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1866 TGGCCA TGG^CCA
MscI 1866 TGGCCA TGG^CCA
NaeI 3948 GCCGGC GCC^GGC
NarI 4142 GGCGCC GG^CGCC
NgoMIV 3948 GCCGGC G^CCGGC
NheI 5110 GCTAGC G^CTAGC
NspV 1773 TTCGAA TT^CGAA
PflFI 4883 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 2180 AACGTT AA^CGTT
PvuI 2168 CGATCG CGAT^CG
SapI 4868 GCTCTTC GCTCTTC(1/4)
SexAI 3812 ACCTGGT A^CC[AT]GGT
SfiI 1745 GGCCTGTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 4142 GGCGCC GGC^GCC
SfuI 1773 TTCGAA TT^CGAA
SpeI 1233 ACTAGT A^CTAGT
SspI 2746 AATATT AAT^ATT
StuI 1744 AGGCCT AGG^CCT
Tth111I 4883 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 2178 GAAACGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 2222 TCCGGA T^CCGGA
4209 TCCGGA
AflIII 1943 ACATGT A^C[GA][CT]GT
3842 ACATGT
BamHI 2452 GGATCC G^GATCC
3639 GGATCC
BclI 2712 TGATCA T^GATCA
3774 TGATCA
BlpI 1487 GCTCAGC GC^T[ACGT]AGC
4966 GCTAAGC
Bpu1102I 1487 GCTCAGC GC^T[ACGT]AGC
4966 GCTAAGC
BsaWI 2222 TCCGGA [AT]^CCGG[AT]
4209 TCCGGA
BseAI 2222 TCCGGA T^CCGGA
4209 TCCGGA
BsiEI 2168 CGATCG CG[GA][CT]^CG
3946 CGGCCG
BsmBI 845 CGTCTC CGTCTC(1/5)
1115 CGTCTC
BsmI 2410 GAATGC GAATGC(1/-1)
3978 GAATGC
BspEI 2222 TCCGGA T^CCGGA
4209 TCCGGA
BspLU11I 1943 ACATGT A^CATGT
3842 ACATGT
BstAPI 243 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4398 GCAGACCTTGC
CelII 1487 GCTCAGC GC^T[ACGT]AGC
4966 GCTAAGC
DrdI 1902 GACCCCGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3223 GACTACTCTGTC
FbaI 2712 TGATCA T^GATCA
3774 TGATCA
HindIII 825 AAGCTT A^AGCTT
1630 AAGCTT
MroI 2222 TCCGGA T^CCGGA
4209 TCCGGA
MspA1I 2270 CAGCTG C[AC]G^C[GT]G
4728 CAGCTG
NcoI 4717 CCATGG C^CATGG
5495 CCATGG
PaeR7I 1833 CTCGAG C^TCGAG
4037 CTCGAG
PflMI 2136 CCAATACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3378 CCAGTGGGTGG
PvuII 2270 CAGCTG CAG^CTG
4728 CAGCTG
ScaI 4441 AGTACT AGT^ACT
5262 AGTACT
TfiI 814 GAATC G^A[AT]TC
4374 GATTC
Van91I 2136 CCAATACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3378 CCAGTGGGTGG
XhoI 1833 CTCGAG C^TCGAG
4037 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
Acc65I AccI AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI ApoI Asp718I AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspHI
BspMI BsrDI BsrFI BsrGI BsrI
BssKI Bst1107I BstF5I BstNI BstXI
BstYI BstZ17I Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoRV EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII