##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AhdI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII BalI
BclI BfrI BsiWI BspDI Bst1107I
BstBI BstZ17I ClaI CpoI Eam1105I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HpaI KpnI KspI MluI
MluNI MscI NdeI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SexAI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Aor51HI 37 AGCGCT AGC^GCT
BcgI 835 GCATCCATATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 2777 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1450 GGTCTC GGTCTC(1/5)
Bsp1407I 1171 TGTACA T^GTACA
BspHI 2952 TCATGA T^CATGA
BspLU11I 4461 ACATGT A^CATGT
BsrDI 1612 CATTGC GCAATG(2/0)
BsrGI 1171 TGTACA T^GTACA
BssHII 138 GCGCGC G^CGCGC
BstAPI 4194 GCAGTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 83 GGTGACC G^GT[ACGT]ACC
BstPI 83 GGTGACC G^GT[ACGT]ACC
BstXI 2149 CCAGCAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 5473 GACTTGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 275 CGGCCG C^GGCCG
EclXI 275 CGGCCG C^GGCCG
Eco47III 37 AGCGCT AGC^GCT
Eco52I 275 CGGCCG C^GGCCG
EcoO65I 83 GGTGACC G^GT[ACGT]ACC
MamI 2777 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3625 CAATTG C^AATTG
MunI 3625 CAATTG C^AATTG
NaeI 239 GCCGGC GCC^GGC
NgoMIV 239 GCCGGC G^CCGGC
PpuMI 4889 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 2952 TCATGA T^CATGA
ScaI 736 AGTACT AGT^ACT
SfiI 276 GGCCGGTTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 181 CCCGGG CCC^GGG
SspBI 1171 TGTACA T^GTACA
SspI 5915 AATATT AAT^ATT
XbaI 2710 TCTAGA T^CTAGA
XmaCI 181 CCCGGG C^CCGGG
XmaI 181 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 3702 TCCGGA T^CCGGA
5469 TCCGGA
AgeI 128 ACCGGT A^CCGGT
4444 ACCGGT
Alw44I 3301 GTGCAC G^TGCAC
4744 GTGCAC
AocI 2165 CCTGAGG CC^T[ACGT]AGG
2941 CCTCAGG
ApaI 190 GGGCCC GGGCC^C
2661 GGGCCC
ApaLI 3301 GTGCAC G^TGCAC
4744 GTGCAC
AvrII 419 CCTAGG C^CTAGG
2745 CCTAGG
BbeI 61 GGCGCC GGCGC^C
925 GGCGCC
BbvCI 4713 CCTCAGC CCTCAGC(-5/-2)
4272 GCTGAGG
BciVI 1215 GGATAC GTATCC(6/5)
3160 GTATCC
BglII 5179 AGATCT A^GATCT
5750 AGATCT
BlnI 419 CCTAGG C^CTAGG
2745 CCTAGG
BseAI 3702 TCCGGA T^CCGGA
5469 TCCGGA
BsiEI 275 CGGCCG CG[GA][CT]^CG
3247 CGACCG
BsmBI 3673 CGTCTC CGTCTC(1/5)
4601 GAGACG
BsmI 2818 GAATGC GAATGC(1/-1)
3228 GCATTC
BspEI 3702 TCCGGA T^CCGGA
5469 TCCGGA
BssSI 660 CACGAG CACGAG(-5/-1)
4220 CTCGTG
Bsu36I 2165 CCTGAGG CC^T[ACGT]AGG
2941 CCTCAGG
DraI 4061 TTTAAA TTT^AAA
4655 TTTAAA
DraIII 756 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
4000 CACTCAGTG
Eco81I 2165 CCTGAGG CC^T[ACGT]AGG
2941 CCTCAGG
HincII 1178 GTTGAC GT[CT]^[GA]AC
1269 GTCAAC
HindII 1178 GTTGAC GT[CT]^[GA]AC
1269 GTCAAC
KasI 61 GGCGCC G^GCGCC
925 GGCGCC
MroI 3702 TCCGGA T^CCGGA
5469 TCCGGA
NarI 61 GGCGCC GG^CGCC
925 GGCGCC
NcoI 2717 CCATGG C^CATGG
4212 CCATGG
NheI 3724 GCTAGC G^CTAGC
3728 GCTAGC
PflMI 869 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
935 CCATCACCTGG
PinAI 128 ACCGGT A^CCGGT
4444 ACCGGT
PshAI 3706 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3917 GACAACTGTC
SacI 3841 GAGCTC GAGCT^C
4071 GAGCTC
SfoI 61 GGCGCC GGC^GCC
925 GGCGCC
TaqI 844 TCGA T^CGA
1363 TCGA
TthHB8I 844 TCGA T^CGA
1363 TCGA
Van91I 869 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
935 CCATCACCTGG
XcmI 3373 CCAGCACTGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4980 CCAGTCAGTCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI Asp700I
AspHI AvaI AvaII BamHI BanI
BanII BbrPI BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PmaCI PmlI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII
XmnI