################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AccIII AclI AgeI AhdI AocI Aor51HI AscI AspEI AviII BbeI BbrPI BcgI BglII BseAI BsiEI BspEI BsrBI BsrFI BssHII BstBI BstEII BstPI Bsu36I Cfr10I CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I FseI FspI HaeII HpaI KasI MluI MroI NaeI NarI NgoMIV NotI NruI NspV PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SwaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AflII 3587 CTTAAG C^TTAAG ApaI 577 GGGCCC GGGCC^C AspI 176 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 2636 GGTCC G^G[AT]CC BalI 226 TGGCCA TGG^CCA BamHI 1378 GGATCC G^GATCC BbvCI 2738 CCTCAGC CCTCAGC(-5/-2) BciVI 4832 GTATCC GTATCC(6/5) BclI 2455 TGATCA T^GATCA BfrI 3587 CTTAAG C^TTAAG BglI 641 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 1044 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 2952 GAGACC GGTCTC(1/5) BsiWI 5731 CGTACG C^GTACG BsmI 3578 GAATGC GAATGC(1/-1) BspDI 2667 ATCGAT AT^CGAT BsrDI 3550 CATTGC GCAATG(2/0) BssSI 1364 CTCGTG CACGAG(-5/-1) Bst1107I 4241 GTATAC GTA^TAC BstXI 6015 CCATTAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1378 GGATCC [GA]^GATC[CT] BstZ17I 4241 GTATAC GTA^TAC ClaI 2667 ATCGAT AT^CGAT DraIII 2062 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 4091 CCTTTAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 5153 GAATTC G^AATTC FbaI 2455 TGATCA T^GATCA KspI 169 CCGCGG CCGC^GG MamI 1044 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 5414 CAATTG C^AATTG MflI 1378 GGATCC [GA]^GATC[CT] MluNI 226 TGGCCA TGG^CCA MscI 226 TGGCCA TGG^CCA MunI 5414 CAATTG C^AATTG NcoI 2611 CCATGG C^CATGG NdeI 1113 CATATG CA^TATG PacI 1724 TTAATTAA TTAAT^TAA PflFI 176 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 2635 GGGTCCT [GA]G^G[AT]CC[CT] PshAI 1999 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 6101 GAGCTC GAGCT^C SacII 169 CCGCGG CCGC^GG SapI 6098 GAAGAGC GCTCTTC(1/4) SmaI 574 CCCGGG CCC^GGG SphI 5696 GCATGC GCATG^C Tth111I 176 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 4538 TCTAGA T^CTAGA XhoII 1378 GGATCC [GA]^GATC[CT] XmaCI 574 CCCGGG C^CCGGG XmaI 574 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AatII 154 GACGTC GACGT^C 2209 GACGTC Acc65I 774 GGTACC G^GTACC 2615 GGTACC AccII 170 CGCG CG^CG 610 CGCG Asp700I 401 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5781 GAACTGTTTC Asp718I 774 GGTACC G^GTACC 2615 GGTACC AvrII 4779 CCTAGG C^CTAGG 5670 CCTAGG BlnI 4779 CCTAGG C^CTAGG 5670 CCTAGG BlpI 1771 GCTCAGC GC^T[ACGT]AGC 4933 GCTGAGC Bpu1102I 1771 GCTCAGC GC^T[ACGT]AGC 4933 GCTGAGC BsaWI 977 ACCGGA [AT]^CCGG[AT] 2859 ACCGGA BsmBI 152 GAGACG CGTCTC(1/5) 3707 GAGACG Bsp1407I 1956 TGTACA T^GTACA 3535 TGTACA BsrGI 1956 TGTACA T^GTACA 3535 TGTACA BstAPI 1850 GCACACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2239 GCACAGATTGC BstUI 170 CGCG CG^CG 610 CGCG CelII 1771 GCTCAGC GC^T[ACGT]AGC 4933 GCTGAGC CfoI 714 GCGC GCG^C 1474 GCGC DraII 577 GGGCCCT [GA]G^G[ACGT]CC[CT] 2635 GGGTCCT DsaI 169 CCGCGG C^C[GA][CT]GG 2611 CCATGG EaeI 226 TGGCCA [CT]^GGCC[GA] 3453 CGGCCA EcoO109I 577 GGGCCCT [GA]G^G[ACGT]CC[CT] 2635 GGGTCCT EcoRV 844 GATATC GAT^ATC 2384 GATATC EcoT22I 4420 ATGCAT ATGCA^T 5698 ATGCAT FauI 4975 CCCGC CCCGC(4/6) 1375 GCGGG HhaI 714 GCGC GCG^C 1474 GCGC HinP1I 714 GCGC G^CGC 1474 GCGC HindIII 253 AAGCTT A^AGCTT 4553 AAGCTT KpnI 774 GGTACC GGTAC^C 2615 GGTACC MvnI 170 CGCG CG^CG 610 CGCG NheI 4058 GCTAGC G^CTAGC 5711 GCTAGC NsiI 4420 ATGCAT ATGCA^T 5698 ATGCAT PflMI 3055 CCAGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3467 CCAAAAACTGG PvuII 1236 CAGCTG CAG^CTG 2741 CAGCTG SspBI 1956 TGTACA T^GTACA 3535 TGTACA Van91I 3055 CCAGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3467 CCAAAAACTGG XmnI 401 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5781 GAACTGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI ApaLI ApoI AseI AsnI AspHI AvaI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspLU11I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI Hin1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PshBI PstI RcaI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I