##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AccIII AclI AgeI AhdI AocI
Aor51HI AscI AspEI AviII BbeI
BbrPI BcgI BglII BseAI BsiEI
BspEI BsrBI BsrFI BssHII BstBI
BstEII BstPI Bsu36I Cfr10I CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I FseI FspI
HaeII HpaI KasI MluI MroI
NaeI NarI NgoMIV NotI NruI
NspV PaeR7I PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AflII 3587 CTTAAG C^TTAAG
ApaI 577 GGGCCC GGGCC^C
AspI 176 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 2636 GGTCC G^G[AT]CC
BalI 226 TGGCCA TGG^CCA
BamHI 1378 GGATCC G^GATCC
BbvCI 2738 CCTCAGC CCTCAGC(-5/-2)
BciVI 4832 GTATCC GTATCC(6/5)
BclI 2455 TGATCA T^GATCA
BfrI 3587 CTTAAG C^TTAAG
BglI 641 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1044 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2952 GAGACC GGTCTC(1/5)
BsiWI 5731 CGTACG C^GTACG
BsmI 3578 GAATGC GAATGC(1/-1)
BspDI 2667 ATCGAT AT^CGAT
BsrDI 3550 CATTGC GCAATG(2/0)
BssSI 1364 CTCGTG CACGAG(-5/-1)
Bst1107I 4241 GTATAC GTA^TAC
BstXI 6015 CCATTAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1378 GGATCC [GA]^GATC[CT]
BstZ17I 4241 GTATAC GTA^TAC
ClaI 2667 ATCGAT AT^CGAT
DraIII 2062 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 4091 CCTTTAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 5153 GAATTC G^AATTC
FbaI 2455 TGATCA T^GATCA
KspI 169 CCGCGG CCGC^GG
MamI 1044 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5414 CAATTG C^AATTG
MflI 1378 GGATCC [GA]^GATC[CT]
MluNI 226 TGGCCA TGG^CCA
MscI 226 TGGCCA TGG^CCA
MunI 5414 CAATTG C^AATTG
NcoI 2611 CCATGG C^CATGG
NdeI 1113 CATATG CA^TATG
PacI 1724 TTAATTAA TTAAT^TAA
PflFI 176 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2635 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1999 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 6101 GAGCTC GAGCT^C
SacII 169 CCGCGG CCGC^GG
SapI 6098 GAAGAGC GCTCTTC(1/4)
SmaI 574 CCCGGG CCC^GGG
SphI 5696 GCATGC GCATG^C
Tth111I 176 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4538 TCTAGA T^CTAGA
XhoII 1378 GGATCC [GA]^GATC[CT]
XmaCI 574 CCCGGG C^CCGGG
XmaI 574 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AatII 154 GACGTC GACGT^C
2209 GACGTC
Acc65I 774 GGTACC G^GTACC
2615 GGTACC
AccII 170 CGCG CG^CG
610 CGCG
Asp700I 401 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5781 GAACTGTTTC
Asp718I 774 GGTACC G^GTACC
2615 GGTACC
AvrII 4779 CCTAGG C^CTAGG
5670 CCTAGG
BlnI 4779 CCTAGG C^CTAGG
5670 CCTAGG
BlpI 1771 GCTCAGC GC^T[ACGT]AGC
4933 GCTGAGC
Bpu1102I 1771 GCTCAGC GC^T[ACGT]AGC
4933 GCTGAGC
BsaWI 977 ACCGGA [AT]^CCGG[AT]
2859 ACCGGA
BsmBI 152 GAGACG CGTCTC(1/5)
3707 GAGACG
Bsp1407I 1956 TGTACA T^GTACA
3535 TGTACA
BsrGI 1956 TGTACA T^GTACA
3535 TGTACA
BstAPI 1850 GCACACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2239 GCACAGATTGC
BstUI 170 CGCG CG^CG
610 CGCG
CelII 1771 GCTCAGC GC^T[ACGT]AGC
4933 GCTGAGC
CfoI 714 GCGC GCG^C
1474 GCGC
DraII 577 GGGCCCT [GA]G^G[ACGT]CC[CT]
2635 GGGTCCT
DsaI 169 CCGCGG C^C[GA][CT]GG
2611 CCATGG
EaeI 226 TGGCCA [CT]^GGCC[GA]
3453 CGGCCA
EcoO109I 577 GGGCCCT [GA]G^G[ACGT]CC[CT]
2635 GGGTCCT
EcoRV 844 GATATC GAT^ATC
2384 GATATC
EcoT22I 4420 ATGCAT ATGCA^T
5698 ATGCAT
FauI 4975 CCCGC CCCGC(4/6)
1375 GCGGG
HhaI 714 GCGC GCG^C
1474 GCGC
HinP1I 714 GCGC G^CGC
1474 GCGC
HindIII 253 AAGCTT A^AGCTT
4553 AAGCTT
KpnI 774 GGTACC GGTAC^C
2615 GGTACC
MvnI 170 CGCG CG^CG
610 CGCG
NheI 4058 GCTAGC G^CTAGC
5711 GCTAGC
NsiI 4420 ATGCAT ATGCA^T
5698 ATGCAT
PflMI 3055 CCAGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3467 CCAAAAACTGG
PvuII 1236 CAGCTG CAG^CTG
2741 CAGCTG
SspBI 1956 TGTACA T^GTACA
3535 TGTACA
Van91I 3055 CCAGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3467 CCAAAAACTGG
XmnI 401 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5781 GAACTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaLI ApoI AseI AsnI AspHI
AvaI BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspLU11I BspMI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI Hin1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PshBI PstI
RcaI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I