################################## # NO CUT ENZYMES ( 57 enzymes) # ################################## AatII Acc65I AclI Aor51HI AscI AseI AsnI Asp700I Asp718I AviII BamHI BclI BspHI BspLU11I BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I CpoI EagI EclXI Eco47III Eco52I EcoO65I EcoRV FbaI FseI FspI HindIII KpnI KspI MfeI MluI MunI NdeI NheI NotI NruI NspV PacI PmeI PshBI Psp1406I RcaI RsrII SacII SalI SfuI SgrAI SnaBI SpeI SphI SwaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AflII 3757 CTTAAG C^TTAAG AflIII 3428 ACGTGT A^C[GA][CT]GT AgeI 196 ACCGGT A^CCGGT AspI 4632 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 2353 TGGCCA TGG^CCA BbsI 459 GAAGAC GAAGAC(2/6) BfrI 3757 CTTAAG C^TTAAG BglII 255 AGATCT A^GATCT BpuAI 459 GAAGAC GAAGAC(2/6) BsaBI 4603 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 4848 CTCCTC GAGGAG(10/8) BsiEI 467 CGATCG CG[GA][CT]^CG BsiWI 2339 CGTACG C^GTACG BsmBI 3077 GAGACG CGTCTC(1/5) BspDI 465 ATCGAT AT^CGAT BsrDI 3372 GCAATG GCAATG(2/0) BssSI 4046 CTCGTG CACGAG(-5/-1) BstAPI 1937 GCAAGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC ClaI 465 ATCGAT AT^CGAT DraI 4802 TTTAAA TTT^AAA DraIII 2624 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoT22I 3052 ATGCAT ATGCA^T MamI 4603 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2353 TGGCCA TGG^CCA MscI 2353 TGGCCA TGG^CCA NaeI 151 GCCGGC GCC^GGC NcoI 823 CCATGG C^CATGG NgoMIV 151 GCCGGC G^CCGGC NsiI 3052 ATGCAT ATGCA^T PflFI 4632 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 196 ACCGGT A^CCGGT PshAI 2227 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 467 CGATCG CGAT^CG SapI 4423 GAAGAGC GCTCTTC(1/4) SbfI 4484 CCTGCAGG CCTGCA^GG ScaI 1111 AGTACT AGT^ACT SexAI 710 ACCAGGT A^CC[AT]GGT SmaI 159 CCCGGG CCC^GGG Sse8387I 4484 CCTGCAGG CCTGCA^GG SspI 3843 AATATT AAT^ATT Tth111I 4632 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2119 TCTAGA T^CTAGA XcmI 1454 CCACCGCTTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 159 CCCGGG C^CCGGG XmaI 159 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccIII 34 TCCGGA T^CCGGA 4353 TCCGGA AvrII 71 CCTAGG C^CTAGG 4266 CCTAGG BbeI 154 GGCGCC GGCGC^C 4882 GGCGCC BbrPI 1689 CACGTG CAC^GTG 4400 CACGTG BbvCI 2191 CCTCAGC CCTCAGC(-5/-2) 3267 GCTGAGG BcgI 354 GCAGGGTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 626 GCAAAGGCCTCG BlnI 71 CCTAGG C^CTAGG 4266 CCTAGG BseAI 34 TCCGGA T^CCGGA 4353 TCCGGA BsmI 972 GCATTC GAATGC(1/-1) 2943 GCATTC BspEI 34 TCCGGA T^CCGGA 4353 TCCGGA BsrFI 151 GCCGGC [GA]^CCGG[CT] 196 ACCGGT Cfr10I 151 GCCGGC [GA]^CCGG[CT] 196 ACCGGT DrdI 1742 GACCACACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2421 GACTGAACTGTC EcoNI 1220 CCTCTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3331 CCTAGCGGAGG HaeII 154 GGCGCC [GA]GCGC^[CT] 4882 GGCGCC HgaI 81 GACGC GACGC(5/10) 114 GACGC HpaI 1927 GTTAAC GTT^AAC 2488 GTTAAC KasI 154 GGCGCC G^GCGCC 4882 GGCGCC MroI 34 TCCGGA T^CCGGA 4353 TCCGGA NarI 154 GGCGCC GG^CGCC 4882 GGCGCC PaeR7I 607 CTCGAG C^TCGAG 931 CTCGAG PflMI 2791 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3638 CCAGGAAATGG PmaCI 1689 CACGTG CAC^GTG 4400 CACGTG PmlI 1689 CACGTG CAC^GTG 4400 CACGTG SfiI 1184 GGCCTTACGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 4076 GGCCTGAGGGGCC SfoI 154 GGCGCC GGC^GCC 4882 GGCGCC Van91I 2791 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3638 CCAGGAAATGG XhoI 607 CTCGAG C^TCGAG 931 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 128 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AhdI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI AspEI AspHI AvaI AvaII BanI BanII BbvI BciVI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaI BsaJI BsaWI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI Eco81I EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII