##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII Acc65I AclI Aor51HI AscI
AseI AsnI Asp700I Asp718I AviII
BamHI BclI BspHI BspLU11I BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I CpoI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV FbaI FseI
FspI HindIII KpnI KspI MfeI
MluI MunI NdeI NheI NotI
NruI NspV PacI PmeI PshBI
Psp1406I RcaI RsrII SacII SalI
SfuI SgrAI SnaBI SpeI SphI
SwaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AflII 3757 CTTAAG C^TTAAG
AflIII 3428 ACGTGT A^C[GA][CT]GT
AgeI 196 ACCGGT A^CCGGT
AspI 4632 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2353 TGGCCA TGG^CCA
BbsI 459 GAAGAC GAAGAC(2/6)
BfrI 3757 CTTAAG C^TTAAG
BglII 255 AGATCT A^GATCT
BpuAI 459 GAAGAC GAAGAC(2/6)
BsaBI 4603 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 4848 CTCCTC GAGGAG(10/8)
BsiEI 467 CGATCG CG[GA][CT]^CG
BsiWI 2339 CGTACG C^GTACG
BsmBI 3077 GAGACG CGTCTC(1/5)
BspDI 465 ATCGAT AT^CGAT
BsrDI 3372 GCAATG GCAATG(2/0)
BssSI 4046 CTCGTG CACGAG(-5/-1)
BstAPI 1937 GCAAGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 465 ATCGAT AT^CGAT
DraI 4802 TTTAAA TTT^AAA
DraIII 2624 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 3052 ATGCAT ATGCA^T
MamI 4603 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2353 TGGCCA TGG^CCA
MscI 2353 TGGCCA TGG^CCA
NaeI 151 GCCGGC GCC^GGC
NcoI 823 CCATGG C^CATGG
NgoMIV 151 GCCGGC G^CCGGC
NsiI 3052 ATGCAT ATGCA^T
PflFI 4632 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 196 ACCGGT A^CCGGT
PshAI 2227 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 467 CGATCG CGAT^CG
SapI 4423 GAAGAGC GCTCTTC(1/4)
SbfI 4484 CCTGCAGG CCTGCA^GG
ScaI 1111 AGTACT AGT^ACT
SexAI 710 ACCAGGT A^CC[AT]GGT
SmaI 159 CCCGGG CCC^GGG
Sse8387I 4484 CCTGCAGG CCTGCA^GG
SspI 3843 AATATT AAT^ATT
Tth111I 4632 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2119 TCTAGA T^CTAGA
XcmI 1454 CCACCGCTTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 159 CCCGGG C^CCGGG
XmaI 159 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 34 TCCGGA T^CCGGA
4353 TCCGGA
AvrII 71 CCTAGG C^CTAGG
4266 CCTAGG
BbeI 154 GGCGCC GGCGC^C
4882 GGCGCC
BbrPI 1689 CACGTG CAC^GTG
4400 CACGTG
BbvCI 2191 CCTCAGC CCTCAGC(-5/-2)
3267 GCTGAGG
BcgI 354 GCAGGGTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
626 GCAAAGGCCTCG
BlnI 71 CCTAGG C^CTAGG
4266 CCTAGG
BseAI 34 TCCGGA T^CCGGA
4353 TCCGGA
BsmI 972 GCATTC GAATGC(1/-1)
2943 GCATTC
BspEI 34 TCCGGA T^CCGGA
4353 TCCGGA
BsrFI 151 GCCGGC [GA]^CCGG[CT]
196 ACCGGT
Cfr10I 151 GCCGGC [GA]^CCGG[CT]
196 ACCGGT
DrdI 1742 GACCACACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2421 GACTGAACTGTC
EcoNI 1220 CCTCTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3331 CCTAGCGGAGG
HaeII 154 GGCGCC [GA]GCGC^[CT]
4882 GGCGCC
HgaI 81 GACGC GACGC(5/10)
114 GACGC
HpaI 1927 GTTAAC GTT^AAC
2488 GTTAAC
KasI 154 GGCGCC G^GCGCC
4882 GGCGCC
MroI 34 TCCGGA T^CCGGA
4353 TCCGGA
NarI 154 GGCGCC GG^CGCC
4882 GGCGCC
PaeR7I 607 CTCGAG C^TCGAG
931 CTCGAG
PflMI 2791 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3638 CCAGGAAATGG
PmaCI 1689 CACGTG CAC^GTG
4400 CACGTG
PmlI 1689 CACGTG CAC^GTG
4400 CACGTG
SfiI 1184 GGCCTTACGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4076 GGCCTGAGGGGCC
SfoI 154 GGCGCC GGC^GCC
4882 GGCGCC
Van91I 2791 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3638 CCAGGAAATGG
XhoI 607 CTCGAG C^TCGAG
931 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AhdI AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI ApoI
AspEI AspHI AvaI AvaII BanI
BanII BbvI BciVI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaJI BsaWI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrBI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI Eam1105I EarI
Eco81I EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII