##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
Aor51HI AscI Asp700I Asp718I AspEI
AspI AviII BbeI BclI BlpI
Bpu1102I BsiWI BsmBI BspDI BssHII
Bst1107I BstZ17I CelII ClaI CpoI
Eam1105I Eco47III EcoNI EcoRV EcoT22I
FbaI FseI FspI HpaI KasI
KpnI MfeI MluI MunI NaeI
NarI NgoMIV NotI NruI NsiI
PacI PflFI PinAI PmeI Psp1406I
PvuI RsrII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI Sse8387I
SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccIII 2539 TCCGGA T^CCGGA
AcyI 5506 GGCGTC G[GA]^CG[CT]C
AflIII 4787 ACATGT A^C[GA][CT]GT
AseI 3156 ATTAAT AT^TAAT
AsnI 3156 ATTAAT AT^TAAT
AvrII 3066 CCTAGG C^CTAGG
BbrPI 3028 CACGTG CAC^GTG
BcgI 3210 CGAATACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 5266 GCCATGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 5397 AGATCT A^GATCT
BlnI 3066 CCTAGG C^CTAGG
BsaBI 4932 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 5506 GGCGTC G[GA]^CG[CT]C
BseAI 2539 TCCGGA T^CCGGA
BsiEI 5248 CGGCCG CG[GA][CT]^CG
BspEI 2539 TCCGGA T^CCGGA
BspLU11I 4787 ACATGT A^CATGT
BsrBI 1329 CCGCTC CCGCTC(-3/-3)
BssSI 6505 CACGAG CACGAG(-5/-1)
BstAPI 5546 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 414 TTCGAA TT^CGAA
EagI 5248 CGGCCG C^GGCCG
EclXI 5248 CGGCCG C^GGCCG
Eco52I 5248 CGGCCG C^GGCCG
EcoRI 3811 GAATTC G^AATTC
Hin1I 5506 GGCGTC G[GA]^CG[CT]C
HincII 6128 GTTGAC GT[CT]^[GA]AC
HindII 6128 GTTGAC GT[CT]^[GA]AC
KspI 1468 CCGCGG CCGC^GG
MamI 4932 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2539 TCCGGA T^CCGGA
NdeI 3991 CATATG CA^TATG
NheI 1161 GCTAGC G^CTAGC
NspV 414 TTCGAA TT^CGAA
PmaCI 3028 CACGTG CAC^GTG
PmlI 3028 CACGTG CAC^GTG
PshAI 5782 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3156 ATTAAT AT^TAAT
SacII 1468 CCGCGG CCGC^GG
SapI 849 GAAGAGC GCTCTTC(1/4)
SfuI 414 TTCGAA TT^CGAA
SpeI 1270 ACTAGT A^CTAGT
StuI 4740 AGGCCT AGG^CCT
XbaI 3744 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 114 CGCG CG^CG
1469 CGCG
AflII 1384 CTTAAG C^TTAAG
1837 CTTAAG
BamHI 374 GGATCC G^GATCC
6327 GGATCC
BfrI 1384 CTTAAG C^TTAAG
1837 CTTAAG
BsgI 1734 CTGCAC GTGCAG(16/14)
2065 CTGCAC
Bsp1407I 46 TGTACA T^GTACA
6525 TGTACA
BspHI 5144 TCATGA T^CATGA
6404 TCATGA
BsrGI 46 TGTACA T^GTACA
6525 TGTACA
BstUI 114 CGCG CG^CG
1469 CGCG
DraI 3405 TTTAAA TTT^AAA
3506 TTTAAA
DrdI 4058 GACAGTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5465 GACTTCCAGGTC
HaeII 556 AGCGCC [GA]GCGC^[CT]
1890 GGCGCT
MvnI 114 CGCG CG^CG
1469 CGCG
PaeR7I 235 CTCGAG C^TCGAG
4716 CTCGAG
RcaI 5144 TCATGA T^CATGA
6404 TCATGA
SacI 3162 GAGCTC GAGCT^C
4140 GAGCTC
SnaBI 975 TACGTA TAC^GTA
4600 TACGTA
SphI 1089 GCATGC GCATG^C
3765 GCATGC
SspBI 46 TGTACA T^GTACA
6525 TGTACA
XcmI 2340 CCAGGGCAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4521 CCAGCCTGACCATGG
XhoI 235 CTCGAG C^TCGAG
4716 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BciVI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstEII BstF5I BstNI BstPI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmaCI XmaI