################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## AatII Acc65I AclI AgeI AhdI Aor51HI AscI Asp700I Asp718I AspEI AspI AviII BbeI BclI BlpI Bpu1102I BsiWI BsmBI BspDI BssHII Bst1107I BstZ17I CelII ClaI CpoI Eam1105I Eco47III EcoNI EcoRV EcoT22I FbaI FseI FspI HpaI KasI KpnI MfeI MluI MunI NaeI NarI NgoMIV NotI NruI NsiI PacI PflFI PinAI PmeI Psp1406I PvuI RsrII SalI SbfI ScaI SexAI SfiI SfoI SgrAI Sse8387I SwaI Tth111I XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccIII 2539 TCCGGA T^CCGGA AcyI 5506 GGCGTC G[GA]^CG[CT]C AflIII 4787 ACATGT A^C[GA][CT]GT AseI 3156 ATTAAT AT^TAAT AsnI 3156 ATTAAT AT^TAAT AvrII 3066 CCTAGG C^CTAGG BbrPI 3028 CACGTG CAC^GTG BcgI 3210 CGAATACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 5266 GCCATGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 5397 AGATCT A^GATCT BlnI 3066 CCTAGG C^CTAGG BsaBI 4932 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 5506 GGCGTC G[GA]^CG[CT]C BseAI 2539 TCCGGA T^CCGGA BsiEI 5248 CGGCCG CG[GA][CT]^CG BspEI 2539 TCCGGA T^CCGGA BspLU11I 4787 ACATGT A^CATGT BsrBI 1329 CCGCTC CCGCTC(-3/-3) BssSI 6505 CACGAG CACGAG(-5/-1) BstAPI 5546 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 414 TTCGAA TT^CGAA EagI 5248 CGGCCG C^GGCCG EclXI 5248 CGGCCG C^GGCCG Eco52I 5248 CGGCCG C^GGCCG EcoRI 3811 GAATTC G^AATTC Hin1I 5506 GGCGTC G[GA]^CG[CT]C HincII 6128 GTTGAC GT[CT]^[GA]AC HindII 6128 GTTGAC GT[CT]^[GA]AC KspI 1468 CCGCGG CCGC^GG MamI 4932 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2539 TCCGGA T^CCGGA NdeI 3991 CATATG CA^TATG NheI 1161 GCTAGC G^CTAGC NspV 414 TTCGAA TT^CGAA PmaCI 3028 CACGTG CAC^GTG PmlI 3028 CACGTG CAC^GTG PshAI 5782 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3156 ATTAAT AT^TAAT SacII 1468 CCGCGG CCGC^GG SapI 849 GAAGAGC GCTCTTC(1/4) SfuI 414 TTCGAA TT^CGAA SpeI 1270 ACTAGT A^CTAGT StuI 4740 AGGCCT AGG^CCT XbaI 3744 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccII 114 CGCG CG^CG 1469 CGCG AflII 1384 CTTAAG C^TTAAG 1837 CTTAAG BamHI 374 GGATCC G^GATCC 6327 GGATCC BfrI 1384 CTTAAG C^TTAAG 1837 CTTAAG BsgI 1734 CTGCAC GTGCAG(16/14) 2065 CTGCAC Bsp1407I 46 TGTACA T^GTACA 6525 TGTACA BspHI 5144 TCATGA T^CATGA 6404 TCATGA BsrGI 46 TGTACA T^GTACA 6525 TGTACA BstUI 114 CGCG CG^CG 1469 CGCG DraI 3405 TTTAAA TTT^AAA 3506 TTTAAA DrdI 4058 GACAGTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5465 GACTTCCAGGTC HaeII 556 AGCGCC [GA]GCGC^[CT] 1890 GGCGCT MvnI 114 CGCG CG^CG 1469 CGCG PaeR7I 235 CTCGAG C^TCGAG 4716 CTCGAG RcaI 5144 TCATGA T^CATGA 6404 TCATGA SacI 3162 GAGCTC GAGCT^C 4140 GAGCTC SnaBI 975 TACGTA TAC^GTA 4600 TACGTA SphI 1089 GCATGC GCATG^C 3765 GCATGC SspBI 46 TGTACA T^GTACA 6525 TGTACA XcmI 2340 CCAGGGCAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4521 CCAGCCTGACCATGG XhoI 235 CTCGAG C^TCGAG 4716 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 130 enzymes) # ######################################### AccI AciI AcsI AfaI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI