##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AccIII AclI AgeI AscI Asp700I
AspI BamHI BbeI BbrPI BglI
BsaAI BseAI BsiEI BsiWI BspDI
BspEI BssHII BssSI BstAPI BstBI
ClaI CpoI EagI EclXI Eco52I
EcoT22I FseI HincII HindII HindIII
HpaI KasI KspI MfeI MluI
MroI MunI NarI NdeI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfoI SfuI SmaI SnaBI
Sse8387I SspI SwaI Tth111I XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 2517 GACGTC GACGT^C
AccII 3449 CGCG CG^CG
Alw44I 4074 GTGCAC G^TGCAC
Aor51HI 4366 AGCGCT AGC^GCT
ApaLI 4074 GTGCAC G^TGCAC
AseI 5067 ATTAAT AT^TAAT
AsnI 5067 ATTAAT AT^TAAT
AviII 1239 TGCGCA TGC^GCA
AvrII 4984 CCTAGG C^CTAGG
BbvCI 2719 GCTGAGG CCTCAGC(-5/-2)
BclI 3521 TGATCA T^GATCA
BlnI 4984 CCTAGG C^CTAGG
BsmBI 729 CGTCTC CGTCTC(1/5)
Bsp1407I 1149 TGTACA T^GTACA
BspLU11I 4895 ACATGT A^CATGT
BsrGI 1149 TGTACA T^GTACA
Bst1107I 1058 GTATAC GTA^TAC
BstEII 4350 GGTGACC G^GT[ACGT]ACC
BstPI 4350 GGTGACC G^GT[ACGT]ACC
BstUI 3449 CGCG CG^CG
BstZ17I 1058 GTATAC GTA^TAC
DraI 4758 TTTAAA TTT^AAA
Eco47III 4366 AGCGCT AGC^GCT
EcoO65I 4350 GGTGACC G^GT[ACGT]ACC
EcoRI 1604 GAATTC G^AATTC
FbaI 3521 TGATCA T^GATCA
FspI 1239 TGCGCA TGC^GCA
MvnI 3449 CGCG CG^CG
NheI 4979 GCTAGC G^CTAGC
PaeR7I 5228 CTCGAG C^TCGAG
PshBI 5067 ATTAAT AT^TAAT
SacI 368 GAGCTC GAGCT^C
ScaI 1840 AGTACT AGT^ACT
SexAI 1053 ACCAGGT A^CC[AT]GGT
SgrAI 446 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 1149 TGTACA T^GTACA
XhoI 5228 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 2579 GGTACC G^GTACC
3241 GGTACC
AccI 1058 GTATAC GT^[AC][GT]AC
5271 GTAGAC
AcyI 1307 GACGCC G[GA]^CG[CT]C
2517 GACGTC
AflII 3305 CTTAAG C^TTAAG
5353 CTTAAG
AhdI 3325 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3879 GACACGGGGTC
ApaI 3943 GGGCCC GGGCC^C
5139 GGGCCC
Asp718I 2579 GGTACC G^GTACC
3241 GGTACC
AspEI 3325 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3879 GACACGGGGTC
BcgI 609 GCAATACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5220 GCACAAAACTCG
BciVI 26 GGATAC GTATCC(6/5)
2781 GTATCC
BfrI 3305 CTTAAG C^TTAAG
5353 CTTAAG
BsaBI 4527 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4603 GATTGACATC
BsaHI 1307 GACGCC G[GA]^CG[CT]C
2517 GACGTC
BsmI 3984 GAATGC GAATGC(1/-1)
3186 GCATTC
DraIII 2710 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
4662 CACCCTGTG
DrdI 1406 GACCCCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2373 GACTCCAGTGTC
Eam1105I 3325 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3879 GACACGGGGTC
EcoNI 1156 CCTAGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3246 CCTTTCCCAGG
EcoRV 1851 GATATC GAT^ATC
4654 GATATC
Hin1I 1307 GACGCC G[GA]^CG[CT]C
2517 GACGTC
KpnI 2579 GGTACC GGTAC^C
3241 GGTACC
MamI 4527 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4603 GATTGACATC
SapI 4881 GAAGAGC GCTCTTC(1/4)
2918 GCTCTTC
SpeI 3375 ACTAGT A^CTAGT
5333 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PshAI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XcmI XhoII