##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AseI AsnI Asp718I AviII
BbeI BbrPI BclI BfrI BsiWI
BsmI BspLU11I BstBI CpoI EagI
EclXI Eco52I EcoT22I FbaI FseI
FspI HincII HindII HpaI KasI
KpnI KspI MfeI MluI MunI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SexAI
SfoI SfuI SgrAI SnaBI SspI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AhdI 5485 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3574 GTGCAC G^TGCAC
ApaI 4163 GGGCCC GGGCC^C
ApaLI 3574 GTGCAC G^TGCAC
AscI 535 GGCGCGCC GG^CGCGCC
Asp700I 3743 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 5485 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2247 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4121 CCTAGG C^CTAGG
BamHI 1991 GGATCC G^GATCC
BbvCI 1656 GCTGAGG CCTCAGC(-5/-2)
BciVI 98 GGATAC GTATCC(6/5)
BlnI 4121 CCTAGG C^CTAGG
BsaAI 5328 CACGTA [CT]AC^GT[GA]
BsaBI 5412 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 6 CGACCG CG[GA][CT]^CG
BspDI 5532 ATCGAT AT^CGAT
BssHII 536 GCGCGC G^CGCGC
Bst1107I 3994 GTATAC GTA^TAC
BstEII 3166 GGTGACC G^GT[ACGT]ACC
BstPI 3166 GGTGACC G^GT[ACGT]ACC
BstXI 83 CCAAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3994 GTATAC GTA^TAC
ClaI 5532 ATCGAT AT^CGAT
Eam1105I 5485 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3166 GGTGACC G^GT[ACGT]ACC
EcoRI 1984 GAATTC G^AATTC
EcoRV 2140 GATATC GAT^ATC
MamI 5412 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 3394 CATATG CA^TATG
PaeR7I 2002 CTCGAG C^TCGAG
PflFI 2247 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SbfI 2073 CCTGCAGG CCTGCA^GG
ScaI 3093 AGTACT AGT^ACT
SfiI 2427 GGCCAAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 936 CCCGGG CCC^GGG
SpeI 2921 ACTAGT A^CTAGT
Sse8387I 2073 CCTGCAGG CCTGCA^GG
SwaI 4330 ATTTAAAT ATTT^AAAT
Tth111I 2247 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 894 TCTAGA T^CTAGA
XhoI 2002 CTCGAG C^TCGAG
XmaCI 936 CCCGGG C^CCGGG
XmaI 936 CCCGGG C^CCGGG
XmnI 3743 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 15 GACGCC G[GA]^CG[CT]C
982 GACGCC
Aor51HI 2256 AGCGCT AGC^GCT
4513 AGCGCT
AvaI 936 CCCGGG C^[CT]CG[GA]G
2002 CTCGAG
BcgI 1023 CGAGAAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5524 GCACACTGATCG
BglI 916 GCCAAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2428 GCCAAGGTGGC
BsaHI 15 GACGCC G[GA]^CG[CT]C
982 GACGCC
BsoBI 936 CCCGGG C^[CT]CG[GA]G
2002 CTCGAG
Bsp1407I 4799 TGTACA T^GTACA
5935 TGTACA
BspHI 2504 TCATGA T^CATGA
3282 TCATGA
BsrDI 218 CATTGC GCAATG(2/0)
1166 CATTGC
BsrFI 1503 GCCGGT [GA]^CCGG[CT]
3294 ACCGGC
BsrGI 4799 TGTACA T^GTACA
5935 TGTACA
Cfr10I 1503 GCCGGT [GA]^CCGG[CT]
3294 ACCGGC
Eco47III 2256 AGCGCT AGC^GCT
4513 AGCGCT
Hin1I 15 GACGCC G[GA]^CG[CT]C
982 GACGCC
NcoI 1676 CCATGG C^CATGG
5150 CCATGG
PshAI 675 GACTATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2732 GACCTTTGTC
RcaI 2504 TCATGA T^CATGA
3282 TCATGA
SphI 861 GCATGC GCATG^C
3854 GCATGC
SspBI 4799 TGTACA T^GTACA
5935 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AfaI AluI AlwI AlwNI AocI
ApoI AspHI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BspEI
BspMI BsrBI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
DrdI DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII