################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AatII Acc65I AclI AflII AflIII AgeI AseI AsnI Asp718I AviII BbeI BbrPI BclI BfrI BsiWI BsmI BspLU11I BstBI CpoI EagI EclXI Eco52I EcoT22I FbaI FseI FspI HincII HindII HpaI KasI KpnI KspI MfeI MluI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SexAI SfoI SfuI SgrAI SnaBI SspI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AhdI 5485 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3574 GTGCAC G^TGCAC ApaI 4163 GGGCCC GGGCC^C ApaLI 3574 GTGCAC G^TGCAC AscI 535 GGCGCGCC GG^CGCGCC Asp700I 3743 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 5485 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2247 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 4121 CCTAGG C^CTAGG BamHI 1991 GGATCC G^GATCC BbvCI 1656 GCTGAGG CCTCAGC(-5/-2) BciVI 98 GGATAC GTATCC(6/5) BlnI 4121 CCTAGG C^CTAGG BsaAI 5328 CACGTA [CT]AC^GT[GA] BsaBI 5412 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 6 CGACCG CG[GA][CT]^CG BspDI 5532 ATCGAT AT^CGAT BssHII 536 GCGCGC G^CGCGC Bst1107I 3994 GTATAC GTA^TAC BstEII 3166 GGTGACC G^GT[ACGT]ACC BstPI 3166 GGTGACC G^GT[ACGT]ACC BstXI 83 CCAAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 3994 GTATAC GTA^TAC ClaI 5532 ATCGAT AT^CGAT Eam1105I 5485 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 3166 GGTGACC G^GT[ACGT]ACC EcoRI 1984 GAATTC G^AATTC EcoRV 2140 GATATC GAT^ATC MamI 5412 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NdeI 3394 CATATG CA^TATG PaeR7I 2002 CTCGAG C^TCGAG PflFI 2247 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC SbfI 2073 CCTGCAGG CCTGCA^GG ScaI 3093 AGTACT AGT^ACT SfiI 2427 GGCCAAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 936 CCCGGG CCC^GGG SpeI 2921 ACTAGT A^CTAGT Sse8387I 2073 CCTGCAGG CCTGCA^GG SwaI 4330 ATTTAAAT ATTT^AAAT Tth111I 2247 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 894 TCTAGA T^CTAGA XhoI 2002 CTCGAG C^TCGAG XmaCI 936 CCCGGG C^CCGGG XmaI 936 CCCGGG C^CCGGG XmnI 3743 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcyI 15 GACGCC G[GA]^CG[CT]C 982 GACGCC Aor51HI 2256 AGCGCT AGC^GCT 4513 AGCGCT AvaI 936 CCCGGG C^[CT]CG[GA]G 2002 CTCGAG BcgI 1023 CGAGAAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 5524 GCACACTGATCG BglI 916 GCCAAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2428 GCCAAGGTGGC BsaHI 15 GACGCC G[GA]^CG[CT]C 982 GACGCC BsoBI 936 CCCGGG C^[CT]CG[GA]G 2002 CTCGAG Bsp1407I 4799 TGTACA T^GTACA 5935 TGTACA BspHI 2504 TCATGA T^CATGA 3282 TCATGA BsrDI 218 CATTGC GCAATG(2/0) 1166 CATTGC BsrFI 1503 GCCGGT [GA]^CCGG[CT] 3294 ACCGGC BsrGI 4799 TGTACA T^GTACA 5935 TGTACA Cfr10I 1503 GCCGGT [GA]^CCGG[CT] 3294 ACCGGC Eco47III 2256 AGCGCT AGC^GCT 4513 AGCGCT Hin1I 15 GACGCC G[GA]^CG[CT]C 982 GACGCC NcoI 1676 CCATGG C^CATGG 5150 CCATGG PshAI 675 GACTATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2732 GACCTTTGTC RcaI 2504 TCATGA T^CATGA 3282 TCATGA SphI 861 GCATGC GCATG^C 3854 GCATGC SspBI 4799 TGTACA T^GTACA 5935 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 133 enzymes) # ######################################### AccI AccII AccIII AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BspEI BspMI BsrBI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DraIII DrdI DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII