##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I ApaLI AscI
Asp718I AspI AvaI AviII BalI
BamHI BbeI BbrPI BciVI BcnI
BfrI BsaAI BsaHI BsaWI BseAI
BsiEI BsiWI BsoBI BspDI BspEI
BsrBI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI EagI EclXI
Eco52I FseI FspI HgaI Hin1I
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MunI NarI
NciI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 3232 CGCG CG^CG
AflIII 3449 ACATGT A^C[GA][CT]GT
AhdI 3301 GACACTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1899 AGCGCT AGC^GCT
ApaI 1741 GGGCCC GGGCC^C
AseI 4214 ATTAAT AT^TAAT
AsnI 4214 ATTAAT AT^TAAT
AspEI 3301 GACACTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3103 CCTAGG C^CTAGG
BcgI 2264 GCAACCTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 489 GCCCCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3850 AGATCT A^GATCT
BlnI 3103 CCTAGG C^CTAGG
BsaBI 1437 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2026 CGTCTC CGTCTC(1/5)
BsmI 5433 GAATGC GAATGC(1/-1)
BspHI 2083 TCATGA T^CATGA
BspLU11I 3449 ACATGT A^CATGT
BsrFI 1832 GCCGGC [GA]^CCGG[CT]
BstUI 3232 CGCG CG^CG
Cfr10I 1832 GCCGGC [GA]^CCGG[CT]
DrdI 1973 GACACTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1830 TGGCCG [CT]^GGCC[GA]
Eam1105I 3301 GACACTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1899 AGCGCT AGC^GCT
EcoT22I 4581 ATGCAT ATGCA^T
FauI 480 CCCGC CCCGC(4/6)
HapII 1833 CCGG C^CGG
HpaI 3477 GTTAAC GTT^AAC
HpaII 1833 CCGG C^CGG
MamI 1437 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 1833 CCGG C^CGG
MvnI 3232 CGCG CG^CG
NaeI 1832 GCCGGC GCC^GGC
NcoI 3152 CCATGG C^CATGG
NgoMIV 1832 GCCGGC G^CCGGC
NsiI 4581 ATGCAT ATGCA^T
PshBI 4214 ATTAAT AT^TAAT
RcaI 2083 TCATGA T^CATGA
SbfI 216 CCTGCAGG CCTGCA^GG
ScaI 5370 AGTACT AGT^ACT
SpeI 2599 ACTAGT A^CTAGT
SphI 4041 GCATGC GCATG^C
Sse8387I 216 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 772 CCTCAGG CC^T[ACGT]AGG
6029 CCTTAGG
BanI 222 GGCACC G^G[CT][GA]CC
2639 GGTGCC
BsaI 5387 GAGACC GGTCTC(1/5)
5681 GAGACC
BspMI 89 ACCTGC ACCTGC(4/8)
4100 GCAGGT
BsrDI 2387 CATTGC GCAATG(2/0)
3278 GCAATG
BstEII 4844 GGTGACC G^GT[ACGT]ACC
5732 GGTTACC
BstPI 4844 GGTGACC G^GT[ACGT]ACC
5732 GGTTACC
BstXI 509 CCAGGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
629 CCAACGTGCTGG
Bsu36I 772 CCTCAGG CC^T[ACGT]AGG
6029 CCTTAGG
DraIII 1534 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
5033 CACTGGGTG
DsaI 783 CCACGG C^C[GA][CT]GG
3152 CCATGG
Eco81I 772 CCTCAGG CC^T[ACGT]AGG
6029 CCTTAGG
EcoO65I 4844 GGTGACC G^GT[ACGT]ACC
5732 GGTTACC
EcoRI 5228 GAATTC G^AATTC
5960 GAATTC
HaeII 1273 AGCGCC [GA]GCGC^[CT]
1899 AGCGCT
HincII 28 GTCAAC GT[CT]^[GA]AC
3477 GTTAAC
HindII 28 GTCAAC GT[CT]^[GA]AC
3477 GTTAAC
PmeI 2147 GTTTAAAC GTTT^AAAC
4877 GTTTAAAC
StuI 2740 AGGCCT AGG^CCT
5861 AGGCCT
XcmI 5473 CCAAGGCTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5719 CCATGATGATAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BanII BbsI BbvCI BbvI
BclI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII EarI EcoNI EcoO109I
EcoRII EcoRV EcoT14I FbaI Fnu4HI
FokI HaeIII HhaI HinP1I HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XhoII XmnI