##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        Alw44I      ApaLI       AscI        
Asp718I     AspI        AvaI        AviII       BalI        
BamHI       BbeI        BbrPI       BciVI       BcnI        
BfrI        BsaAI       BsaHI       BsaWI       BseAI       
BsiEI       BsiWI       BsoBI       BspDI       BspEI       
BsrBI       BssHII      BssSI       Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        EagI        EclXI       
Eco52I      FseI        FspI        HgaI        Hin1I       
KasI        KpnI        KspI        MfeI        MluI        
MluNI       MroI        MscI        MunI        NarI        
NciI        NdeI        NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmlI        PshAI       Psp1406I    PvuI        
RsrII       SacII       SalI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SwaI        Tth111I     XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccII        3232  CGCG                 CG^CG
AflIII       3449  ACATGT               A^C[GA][CT]GT
AhdI         3301  GACACTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      1899  AGCGCT               AGC^GCT
ApaI         1741  GGGCCC               GGGCC^C
AseI         4214  ATTAAT               AT^TAAT
AsnI         4214  ATTAAT               AT^TAAT
AspEI        3301  GACACTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        3103  CCTAGG               C^CTAGG
BcgI         2264  GCAACCTTCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          489  GCCCCACAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        3850  AGATCT               A^GATCT
BlnI         3103  CCTAGG               C^CTAGG
BsaBI        1437  GATAGCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        2026  CGTCTC               CGTCTC(1/5)
BsmI         5433  GAATGC               GAATGC(1/-1)
BspHI        2083  TCATGA               T^CATGA
BspLU11I     3449  ACATGT               A^CATGT
BsrFI        1832  GCCGGC               [GA]^CCGG[CT]
BstUI        3232  CGCG                 CG^CG
Cfr10I       1832  GCCGGC               [GA]^CCGG[CT]
DrdI         1973  GACACTTTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         1830  TGGCCG               [CT]^GGCC[GA]
Eam1105I     3301  GACACTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1899  AGCGCT               AGC^GCT
EcoT22I      4581  ATGCAT               ATGCA^T
FauI          480  CCCGC                CCCGC(4/6)
HapII        1833  CCGG                 C^CGG
HpaI         3477  GTTAAC               GTT^AAC
HpaII        1833  CCGG                 C^CGG
MamI         1437  GATAGCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI         1833  CCGG                 C^CGG
MvnI         3232  CGCG                 CG^CG
NaeI         1832  GCCGGC               GCC^GGC
NcoI         3152  CCATGG               C^CATGG
NgoMIV       1832  GCCGGC               G^CCGGC
NsiI         4581  ATGCAT               ATGCA^T
PshBI        4214  ATTAAT               AT^TAAT
RcaI         2083  TCATGA               T^CATGA
SbfI          216  CCTGCAGG             CCTGCA^GG
ScaI         5370  AGTACT               AGT^ACT
SpeI         2599  ACTAGT               A^CTAGT
SphI         4041  GCATGC               GCATG^C
Sse8387I      216  CCTGCAGG             CCTGCA^GG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AocI          772  CCTCAGG              CC^T[ACGT]AGG
             6029  CCTTAGG             
BanI          222  GGCACC               G^G[CT][GA]CC
             2639  GGTGCC              
BsaI         5387  GAGACC               GGTCTC(1/5)
             5681  GAGACC              
BspMI          89  ACCTGC               ACCTGC(4/8)
             4100  GCAGGT              
BsrDI        2387  CATTGC               GCAATG(2/0)
             3278  GCAATG              
BstEII       4844  GGTGACC              G^GT[ACGT]ACC
             5732  GGTTACC             
BstPI        4844  GGTGACC              G^GT[ACGT]ACC
             5732  GGTTACC             
BstXI         509  CCAGGAGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              629  CCAACGTGCTGG        
Bsu36I        772  CCTCAGG              CC^T[ACGT]AGG
             6029  CCTTAGG             
DraIII       1534  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             5033  CACTGGGTG           
DsaI          783  CCACGG               C^C[GA][CT]GG
             3152  CCATGG              
Eco81I        772  CCTCAGG              CC^T[ACGT]AGG
             6029  CCTTAGG             
EcoO65I      4844  GGTGACC              G^GT[ACGT]ACC
             5732  GGTTACC             
EcoRI        5228  GAATTC               G^AATTC
             5960  GAATTC              
HaeII        1273  AGCGCC               [GA]GCGC^[CT]
             1899  AGCGCT              
HincII         28  GTCAAC               GT[CT]^[GA]AC
             3477  GTTAAC              
HindII         28  GTCAAC               GT[CT]^[GA]AC
             3477  GTTAAC              
PmeI         2147  GTTTAAAC             GTTT^AAAC
             4877  GTTTAAAC            
StuI         2740  AGGCCT               AGG^CCT
             5861  AGGCCT              
XcmI         5473  CCAAGGCTTCTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             5719  CCATGATGATAATGG     


#########################################
# 3 OR MORE CUT ENZYMES (  109 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaII       BanII       BbsI        BbvCI       BbvI        
BclI        BfaI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    Bsp1407I    BsrGI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstYI       Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       EarI        EcoNI       EcoO109I    
EcoRII      EcoRV       EcoT14I     FbaI        Fnu4HI      
FokI        HaeIII      HhaI        HinP1I      HindIII     
HinfI       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        SacI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SspBI       
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XbaI        XhoII       XmnI