################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI Alw44I ApaLI AscI Asp718I AspI AvaI AviII BalI BamHI BbeI BbrPI BciVI BcnI BfrI BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsoBI BspDI BspEI BsrBI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI EagI EclXI Eco52I FseI FspI HgaI Hin1I KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI NarI NciI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccII 3232 CGCG CG^CG AflIII 3449 ACATGT A^C[GA][CT]GT AhdI 3301 GACACTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1899 AGCGCT AGC^GCT ApaI 1741 GGGCCC GGGCC^C AseI 4214 ATTAAT AT^TAAT AsnI 4214 ATTAAT AT^TAAT AspEI 3301 GACACTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 3103 CCTAGG C^CTAGG BcgI 2264 GCAACCTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 489 GCCCCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3850 AGATCT A^GATCT BlnI 3103 CCTAGG C^CTAGG BsaBI 1437 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 2026 CGTCTC CGTCTC(1/5) BsmI 5433 GAATGC GAATGC(1/-1) BspHI 2083 TCATGA T^CATGA BspLU11I 3449 ACATGT A^CATGT BsrFI 1832 GCCGGC [GA]^CCGG[CT] BstUI 3232 CGCG CG^CG Cfr10I 1832 GCCGGC [GA]^CCGG[CT] DrdI 1973 GACACTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 1830 TGGCCG [CT]^GGCC[GA] Eam1105I 3301 GACACTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1899 AGCGCT AGC^GCT EcoT22I 4581 ATGCAT ATGCA^T FauI 480 CCCGC CCCGC(4/6) HapII 1833 CCGG C^CGG HpaI 3477 GTTAAC GTT^AAC HpaII 1833 CCGG C^CGG MamI 1437 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspI 1833 CCGG C^CGG MvnI 3232 CGCG CG^CG NaeI 1832 GCCGGC GCC^GGC NcoI 3152 CCATGG C^CATGG NgoMIV 1832 GCCGGC G^CCGGC NsiI 4581 ATGCAT ATGCA^T PshBI 4214 ATTAAT AT^TAAT RcaI 2083 TCATGA T^CATGA SbfI 216 CCTGCAGG CCTGCA^GG ScaI 5370 AGTACT AGT^ACT SpeI 2599 ACTAGT A^CTAGT SphI 4041 GCATGC GCATG^C Sse8387I 216 CCTGCAGG CCTGCA^GG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AocI 772 CCTCAGG CC^T[ACGT]AGG 6029 CCTTAGG BanI 222 GGCACC G^G[CT][GA]CC 2639 GGTGCC BsaI 5387 GAGACC GGTCTC(1/5) 5681 GAGACC BspMI 89 ACCTGC ACCTGC(4/8) 4100 GCAGGT BsrDI 2387 CATTGC GCAATG(2/0) 3278 GCAATG BstEII 4844 GGTGACC G^GT[ACGT]ACC 5732 GGTTACC BstPI 4844 GGTGACC G^GT[ACGT]ACC 5732 GGTTACC BstXI 509 CCAGGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 629 CCAACGTGCTGG Bsu36I 772 CCTCAGG CC^T[ACGT]AGG 6029 CCTTAGG DraIII 1534 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG 5033 CACTGGGTG DsaI 783 CCACGG C^C[GA][CT]GG 3152 CCATGG Eco81I 772 CCTCAGG CC^T[ACGT]AGG 6029 CCTTAGG EcoO65I 4844 GGTGACC G^GT[ACGT]ACC 5732 GGTTACC EcoRI 5228 GAATTC G^AATTC 5960 GAATTC HaeII 1273 AGCGCC [GA]GCGC^[CT] 1899 AGCGCT HincII 28 GTCAAC GT[CT]^[GA]AC 3477 GTTAAC HindII 28 GTCAAC GT[CT]^[GA]AC 3477 GTTAAC PmeI 2147 GTTTAAAC GTTT^AAAC 4877 GTTTAAAC StuI 2740 AGGCCT AGG^CCT 5861 AGGCCT XcmI 5473 CCAAGGCTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5719 CCATGATGATAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BanII BbsI BbvCI BbvI BclI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII EarI EcoNI EcoO109I EcoRII EcoRV EcoT14I FbaI Fnu4HI FokI HaeIII HhaI HinP1I HindIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XhoII XmnI