################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII AclI AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AspI AviII BclI BsaBI BsiWI BspDI BspHI BsrFI BssHII Bst1107I BstAPI BstBI BstZ17I Cfr10I ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV FbaI FseI FspI MamI MfeI MluI MunI NaeI NgoMIV NheI NotI NruI NspV PacI PflFI PinAI PmeI PshBI Psp1406I RcaI RsrII SalI SfiI SfuI SgrAI SnaBI SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 4962 GGTACC G^GTACC AccIII 5241 TCCGGA T^CCGGA AocI 936 CCTGAGG CC^T[ACGT]AGG Asp718I 4962 GGTACC G^GTACC BseAI 5241 TCCGGA T^CCGGA BsgI 1605 CTGCAC GTGCAG(16/14) BsiEI 5009 CGATCG CG[GA][CT]^CG BsmBI 5776 CGTCTC CGTCTC(1/5) Bsp1407I 1287 TGTACA T^GTACA BspEI 5241 TCCGGA T^CCGGA BspLU11I 4720 ACATGT A^CATGT BsrGI 1287 TGTACA T^GTACA BssSI 1540 CACGAG CACGAG(-5/-1) BstEII 5275 GGTCACC G^GT[ACGT]ACC BstPI 5275 GGTCACC G^GT[ACGT]ACC BstXI 2897 CCAGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 936 CCTGAGG CC^T[ACGT]AGG DrdI 3919 GACGGGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 936 CCTGAGG CC^T[ACGT]AGG EcoO65I 5275 GGTCACC G^GT[ACGT]ACC EcoT22I 4891 ATGCAT ATGCA^T HgaI 5387 GCGTC GACGC(5/10) HindIII 2442 AAGCTT A^AGCTT HpaI 4245 GTTAAC GTT^AAC KpnI 4962 GGTACC GGTAC^C KspI 4618 CCGCGG CCGC^GG MroI 5241 TCCGGA T^CCGGA NdeI 387 CATATG CA^TATG NsiI 4891 ATGCAT ATGCA^T PaeR7I 4541 CTCGAG C^TCGAG PshAI 841 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 5009 CGATCG CGAT^CG SacII 4618 CCGCGG CCGC^GG SbfI 5158 CCTGCAGG CCTGCA^GG ScaI 3247 AGTACT AGT^ACT SexAI 60 ACCAGGT A^CC[AT]GGT SpeI 3607 ACTAGT A^CTAGT Sse8387I 5158 CCTGCAGG CCTGCA^GG SspBI 1287 TGTACA T^GTACA SspI 3507 AATATT AAT^ATT XhoI 4541 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AccII 2197 CGCG CG^CG 4619 CGCG AcyI 1099 GGCGCC G[GA]^CG[CT]C 1750 GGCGCC AflII 1955 CTTAAG C^TTAAG 3766 CTTAAG AflIII 4366 ACGTGT A^C[GA][CT]GT 4720 ACATGT AlwNI 1246 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG 1348 CAGTTCCTG BamHI 2703 GGATCC G^GATCC 5805 GGATCC BbeI 1099 GGCGCC GGCGC^C 1750 GGCGCC BbrPI 781 CACGTG CAC^GTG 4365 CACGTG BbsI 4655 GAAGAC GAAGAC(2/6) 3562 GTCTTC BciVI 3180 GTATCC GTATCC(6/5) 4804 GTATCC BfrI 1955 CTTAAG C^TTAAG 3766 CTTAAG BglI 745 GCCCTTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1076 GCCACCGTGGC BglII 1205 AGATCT A^GATCT 5127 AGATCT BpuAI 4655 GAAGAC GAAGAC(2/6) 3562 GTCTTC BsaAI 781 CACGTG [CT]AC^GT[GA] 4365 CACGTG BsaHI 1099 GGCGCC G[GA]^CG[CT]C 1750 GGCGCC BsaWI 1719 TCCGGT [AT]^CCGG[AT] 5241 TCCGGA BsrDI 2257 GCAATG GCAATG(2/0) 3407 GCAATG BstUI 2197 CGCG CG^CG 4619 CGCG DraI 1685 TTTAAA TTT^AAA 2319 TTTAAA Hin1I 1099 GGCGCC G[GA]^CG[CT]C 1750 GGCGCC HincII 2608 GTCAAC GT[CT]^[GA]AC 4245 GTTAAC HindII 2608 GTCAAC GT[CT]^[GA]AC 4245 GTTAAC KasI 1099 GGCGCC G^GCGCC 1750 GGCGCC MvnI 2197 CGCG CG^CG 4619 CGCG NarI 1099 GGCGCC GG^CGCC 1750 GGCGCC NcoI 4375 CCATGG C^CATGG 5212 CCATGG PmaCI 781 CACGTG CAC^GTG 4365 CACGTG PmlI 781 CACGTG CAC^GTG 4365 CACGTG PstI 227 CTGCAG CTGCA^G 5159 CTGCAG SacI 361 GAGCTC GAGCT^C 1547 GAGCTC SfoI 1099 GGCGCC GGC^GCC 1750 GGCGCC SmaI 828 CCCGGG CCC^GGG 4307 CCCGGG SphI 2812 GCATGC GCATG^C 3324 GCATGC StuI 3103 AGGCCT AGG^CCT 5554 AGGCCT XmaCI 828 CCCGGG C^CCGGG 4307 CCCGGG XmaI 828 CCCGGG C^CCGGG 4307 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI ApaI ApoI AspHI AvaI AvaII AvrII BalI BanI BanII BbvCI BbvI BcgI BcnI BfaI BlnI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII