##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AclI AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AviII BclI
BsaBI BsiWI BspDI BspHI BsrFI
BssHII Bst1107I BstAPI BstBI BstZ17I
Cfr10I ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRI EcoRV FbaI FseI FspI
MamI MfeI MluI MunI NaeI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I RcaI RsrII SalI SfiI
SfuI SgrAI SnaBI SwaI Tth111I
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 4962 GGTACC G^GTACC
AccIII 5241 TCCGGA T^CCGGA
AocI 936 CCTGAGG CC^T[ACGT]AGG
Asp718I 4962 GGTACC G^GTACC
BseAI 5241 TCCGGA T^CCGGA
BsgI 1605 CTGCAC GTGCAG(16/14)
BsiEI 5009 CGATCG CG[GA][CT]^CG
BsmBI 5776 CGTCTC CGTCTC(1/5)
Bsp1407I 1287 TGTACA T^GTACA
BspEI 5241 TCCGGA T^CCGGA
BspLU11I 4720 ACATGT A^CATGT
BsrGI 1287 TGTACA T^GTACA
BssSI 1540 CACGAG CACGAG(-5/-1)
BstEII 5275 GGTCACC G^GT[ACGT]ACC
BstPI 5275 GGTCACC G^GT[ACGT]ACC
BstXI 2897 CCAGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 936 CCTGAGG CC^T[ACGT]AGG
DrdI 3919 GACGGGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 936 CCTGAGG CC^T[ACGT]AGG
EcoO65I 5275 GGTCACC G^GT[ACGT]ACC
EcoT22I 4891 ATGCAT ATGCA^T
HgaI 5387 GCGTC GACGC(5/10)
HindIII 2442 AAGCTT A^AGCTT
HpaI 4245 GTTAAC GTT^AAC
KpnI 4962 GGTACC GGTAC^C
KspI 4618 CCGCGG CCGC^GG
MroI 5241 TCCGGA T^CCGGA
NdeI 387 CATATG CA^TATG
NsiI 4891 ATGCAT ATGCA^T
PaeR7I 4541 CTCGAG C^TCGAG
PshAI 841 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 5009 CGATCG CGAT^CG
SacII 4618 CCGCGG CCGC^GG
SbfI 5158 CCTGCAGG CCTGCA^GG
ScaI 3247 AGTACT AGT^ACT
SexAI 60 ACCAGGT A^CC[AT]GGT
SpeI 3607 ACTAGT A^CTAGT
Sse8387I 5158 CCTGCAGG CCTGCA^GG
SspBI 1287 TGTACA T^GTACA
SspI 3507 AATATT AAT^ATT
XhoI 4541 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccII 2197 CGCG CG^CG
4619 CGCG
AcyI 1099 GGCGCC G[GA]^CG[CT]C
1750 GGCGCC
AflII 1955 CTTAAG C^TTAAG
3766 CTTAAG
AflIII 4366 ACGTGT A^C[GA][CT]GT
4720 ACATGT
AlwNI 1246 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1348 CAGTTCCTG
BamHI 2703 GGATCC G^GATCC
5805 GGATCC
BbeI 1099 GGCGCC GGCGC^C
1750 GGCGCC
BbrPI 781 CACGTG CAC^GTG
4365 CACGTG
BbsI 4655 GAAGAC GAAGAC(2/6)
3562 GTCTTC
BciVI 3180 GTATCC GTATCC(6/5)
4804 GTATCC
BfrI 1955 CTTAAG C^TTAAG
3766 CTTAAG
BglI 745 GCCCTTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1076 GCCACCGTGGC
BglII 1205 AGATCT A^GATCT
5127 AGATCT
BpuAI 4655 GAAGAC GAAGAC(2/6)
3562 GTCTTC
BsaAI 781 CACGTG [CT]AC^GT[GA]
4365 CACGTG
BsaHI 1099 GGCGCC G[GA]^CG[CT]C
1750 GGCGCC
BsaWI 1719 TCCGGT [AT]^CCGG[AT]
5241 TCCGGA
BsrDI 2257 GCAATG GCAATG(2/0)
3407 GCAATG
BstUI 2197 CGCG CG^CG
4619 CGCG
DraI 1685 TTTAAA TTT^AAA
2319 TTTAAA
Hin1I 1099 GGCGCC G[GA]^CG[CT]C
1750 GGCGCC
HincII 2608 GTCAAC GT[CT]^[GA]AC
4245 GTTAAC
HindII 2608 GTCAAC GT[CT]^[GA]AC
4245 GTTAAC
KasI 1099 GGCGCC G^GCGCC
1750 GGCGCC
MvnI 2197 CGCG CG^CG
4619 CGCG
NarI 1099 GGCGCC GG^CGCC
1750 GGCGCC
NcoI 4375 CCATGG C^CATGG
5212 CCATGG
PmaCI 781 CACGTG CAC^GTG
4365 CACGTG
PmlI 781 CACGTG CAC^GTG
4365 CACGTG
PstI 227 CTGCAG CTGCA^G
5159 CTGCAG
SacI 361 GAGCTC GAGCT^C
1547 GAGCTC
SfoI 1099 GGCGCC GGC^GCC
1750 GGCGCC
SmaI 828 CCCGGG CCC^GGG
4307 CCCGGG
SphI 2812 GCATGC GCATG^C
3324 GCATGC
StuI 3103 AGGCCT AGG^CCT
5554 AGGCCT
XmaCI 828 CCCGGG C^CCGGG
4307 CCCGGG
XmaI 828 CCCGGG C^CCGGG
4307 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApaI ApoI AspHI AvaI
AvaII AvrII BalI BanI BanII
BbvCI BbvI BcgI BcnI BfaI
BlnI BlpI BmyI BpmI Bpu1102I
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII