##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
Acc65I AccII AccIII Alw44I Aor51HI
ApaLI AscI Asp700I Asp718I AspI
AviII AvrII BamHI BanI BbeI
BbrPI BglI BlnI BseAI BsiEI
BsiWI BspDI BspEI BsrBI BssHII
BstBI BstEII BstPI BstUI BstXI
CfoI ClaI CpoI EagI EclXI
Eco47III Eco52I EcoNI EcoO65I FseI
FspI HaeII HhaI HinP1I HpaI
KasI KpnI KspI MluI MroI
MvnI NaeI NarI NgoMIV NotI
NruI NspV PacI PflFI PmaCI
PmeI PmlI PshAI PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 2823 GACGTC GACGT^C
AclI 5011 AACGTT AA^CGTT
AgeI 834 ACCGGT A^CCGGT
AhdI 2350 GACTAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2032 CCTCAGG CC^T[ACGT]AGG
ApaI 108 GGGCCC GGGCC^C
AspEI 2350 GACTAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 1089 GCTGAGG CCTCAGC(-5/-2)
BcgI 4338 GCACTACTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4799 GGATAC GTATCC(6/5)
BlpI 4515 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4515 GCTGAGC GC^T[ACGT]AGC
BsaAI 646 TACGTG [CT]AC^GT[GA]
BsaBI 1008 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 834 ACCGGT [AT]^CCGG[AT]
BsrFI 834 ACCGGT [GA]^CCGG[CT]
BssSI 3679 CACGAG CACGAG(-5/-1)
Bst1107I 3333 GTATAC GTA^TAC
BstAPI 4736 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3333 GTATAC GTA^TAC
Bsu36I 2032 CCTCAGG CC^T[ACGT]AGG
CelII 4515 GCTGAGC GC^T[ACGT]AGC
Cfr10I 834 ACCGGT [GA]^CCGG[CT]
DraII 1094 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 583 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2350 GACTAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2032 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1094 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 720 GATATC GAT^ATC
EcoT22I 2025 ATGCAT ATGCA^T
HincII 4764 GTTGAC GT[CT]^[GA]AC
HindII 4764 GTTGAC GT[CT]^[GA]AC
HindIII 1895 AAGCTT A^AGCTT
MamI 1008 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5060 CAATTG C^AATTG
MunI 5060 CAATTG C^AATTG
NcoI 1408 CCATGG C^CATGG
NdeI 2548 CATATG CA^TATG
NheI 1224 GCTAGC G^CTAGC
NsiI 2025 ATGCAT ATGCA^T
PaeR7I 3916 CTCGAG C^TCGAG
PflMI 99 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 834 ACCGGT A^CCGGT
PpuMI 1094 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 5011 AACGTT AA^CGTT
SacI 701 GAGCTC GAGCT^C
SapI 222 GAAGAGC GCTCTTC(1/4)
SexAI 3556 ACCAGGT A^CC[AT]GGT
SmaI 1216 CCCGGG CCC^GGG
StuI 1432 AGGCCT AGG^CCT
SwaI 5070 ATTTAAAT ATTT^AAAT
TaqI 3917 TCGA T^CGA
TthHB8I 3917 TCGA T^CGA
Van91I 99 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2332 CCATACTATAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3916 CTCGAG C^TCGAG
XmaCI 1216 CCCGGG C^CCGGG
XmaI 1216 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflIII 2949 ACATGT A^C[GA][CT]GT
4051 ACATGT
AlwNI 489 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
4737 CAGAGCCTG
AspHI 299 GTGCTC G[AT]GC[AT]^C
701 GAGCTC
BsaI 67 GAGACC GGTCTC(1/5)
3890 GGTCTC
BsiHKAI 299 GTGCTC G[AT]GC[AT]^C
701 GAGCTC
BsmBI 4654 GAGACG CGTCTC(1/5)
4883 GAGACG
BsmI 250 GAATGC GAATGC(1/-1)
1526 GAATGC
BspHI 1289 TCATGA T^CATGA
1616 TCATGA
BspLU11I 2949 ACATGT A^CATGT
4051 ACATGT
BspMI 1186 GCAGGT ACCTGC(4/8)
3996 GCAGGT
EcoRI 2307 GAATTC G^AATTC
4751 GAATTC
FauI 4243 CCCGC CCCGC(4/6)
30 GCGGG
MslI 2602 CATTGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2733 CATGCAAATG
PstI 308 CTGCAG CTGCA^G
654 CTGCAG
RcaI 1289 TCATGA T^CATGA
1616 TCATGA
SpeI 2523 ACTAGT A^CTAGT
3770 ACTAGT
SphI 2027 GCATGC GCATG^C
2732 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflII AluI AlwI ApoI AseI
AsnI AvaI AvaII BalI BanII
BbsI BbvI BclI BcnI BfaI
BfrI BglII BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DrdI DsaI
EaeI EarI EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HgaI
Hin1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PshBI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XbaI
XhoII