################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AhdI ApaI AscI Asp718I AspEI AviII AvrII BbeI BciVI BclI BlnI BsaBI BseAI BsiWI BspDI BspEI BssHII BssSI BstAPI BstUI ClaI CpoI Eam1105I EcoRI EcoRV FbaI FseI FspI HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NotI NruI PacI PaeR7I PflMI PmeI PshAI Psp1406I RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SnaBI SpeI Sse8387I Van91I XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcyI 870 GACGCC G[GA]^CG[CT]C AgeI 3040 ACCGGT A^CCGGT Alw44I 3160 GTGCAC G^TGCAC ApaLI 3160 GTGCAC G^TGCAC AseI 4132 ATTAAT AT^TAAT AsnI 4132 ATTAAT AT^TAAT AspI 2903 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 845 CACGTG CAC^GTG BbsI 3658 GTCTTC GAAGAC(2/6) BcgI 924 CGAGTGGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 4863 AGATCT A^GATCT BpuAI 3658 GTCTTC GAAGAC(2/6) BsaHI 870 GACGCC G[GA]^CG[CT]C BspHI 1488 TCATGA T^CATGA BspLU11I 5089 ACATGT A^CATGT BsrFI 3040 ACCGGT [GA]^CCGG[CT] Bst1107I 4230 GTATAC GTA^TAC BstBI 2 TTCGAA TT^CGAA BstEII 2303 GGTCACC G^GT[ACGT]ACC BstPI 2303 GGTCACC G^GT[ACGT]ACC BstZ17I 4230 GTATAC GTA^TAC Cfr10I 3040 ACCGGT [GA]^CCGG[CT] DraIII 817 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 2517 CGGCCG C^GGCCG EclXI 2517 CGGCCG C^GGCCG Eco52I 2517 CGGCCG C^GGCCG EcoO65I 2303 GGTCACC G^GT[ACGT]ACC Hin1I 870 GACGCC G[GA]^CG[CT]C HincII 4857 GTTGAC GT[CT]^[GA]AC HindII 4857 GTTGAC GT[CT]^[GA]AC NheI 3850 GCTAGC G^CTAGC NspV 2 TTCGAA TT^CGAA PflFI 2903 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 3040 ACCGGT A^CCGGT PmaCI 845 CACGTG CAC^GTG PmlI 845 CACGTG CAC^GTG PshBI 4132 ATTAAT AT^TAAT PvuI 380 CGATCG CGAT^CG RcaI 1488 TCATGA T^CATGA SfuI 2 TTCGAA TT^CGAA SmaI 1447 CCCGGG CCC^GGG SphI 1086 GCATGC GCATG^C SwaI 4219 ATTTAAAT ATTT^AAAT Tth111I 2903 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 3199 TCTAGA T^CTAGA XmaCI 1447 CCCGGG C^CCGGG XmaI 1447 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 4230 GTATAC GT^[AC][GT]AC 5212 GTAGAC AflII 2820 CTTAAG C^TTAAG 5076 CTTAAG AflIII 844 ACACGT A^C[GA][CT]GT 5089 ACATGT Aor51HI 3802 AGCGCT AGC^GCT 5199 AGCGCT BamHI 2595 GGATCC G^GATCC 4459 GGATCC BanI 1226 GGTGCC G^G[CT][GA]CC 2604 GGTGCC BbvCI 560 CCTCAGC CCTCAGC(-5/-2) 1234 CCTCAGC BfrI 2820 CTTAAG C^TTAAG 5076 CTTAAG BglI 2621 GCCACCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3617 GCCCAAAAGGC BsaAI 845 CACGTG [CT]AC^GT[GA] 3225 CACGTA BsiEI 380 CGATCG CG[GA][CT]^CG 2517 CGGCCG BsmBI 1985 CGTCTC CGTCTC(1/5) 2513 GAGACG BsmI 2709 GAATGC GAATGC(1/-1) 199 GCATTC BsrBI 771 CCGCTC CCGCTC(-3/-3) 2334 CCGCTC DrdI 3599 GACCCTCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4901 GACAGGTCTGTC Eco47III 3802 AGCGCT AGC^GCT 5199 AGCGCT EcoNI 796 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3530 CCTCCGCTAGG EcoT22I 4065 ATGCAT ATGCA^T 4082 ATGCAT FauI 177 CCCGC CCCGC(4/6) 754 CCCGC HgaI 870 GACGC GACGC(5/10) 2110 GACGC NcoI 155 CCATGG C^CATGG 2207 CCATGG NsiI 4065 ATGCAT ATGCA^T 4082 ATGCAT NspI 1086 GCATGC [GA]CATG^[CT] 5089 ACATGT SacI 2325 GAGCTC GAGCT^C 2677 GAGCTC SapI 327 GAAGAGC GCTCTTC(1/4) 505 GCTCTTC ScaI 3483 AGTACT AGT^ACT 5072 AGTACT TfiI 3489 GAATC G^A[AT]TC 4711 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaI AvaII BalI BanII BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI