##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AhdI ApaI AscI Asp718I AspEI
AviII AvrII BbeI BciVI BclI
BlnI BsaBI BseAI BsiWI BspDI
BspEI BssHII BssSI BstAPI BstUI
ClaI CpoI Eam1105I EcoRI EcoRV
FbaI FseI FspI HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NdeI NgoMIV NotI NruI PacI
PaeR7I PflMI PmeI PshAI Psp1406I
RsrII SacII SalI SbfI SexAI
SfiI SfoI SgrAI SnaBI SpeI
Sse8387I Van91I XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AcyI 870 GACGCC G[GA]^CG[CT]C
AgeI 3040 ACCGGT A^CCGGT
Alw44I 3160 GTGCAC G^TGCAC
ApaLI 3160 GTGCAC G^TGCAC
AseI 4132 ATTAAT AT^TAAT
AsnI 4132 ATTAAT AT^TAAT
AspI 2903 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 845 CACGTG CAC^GTG
BbsI 3658 GTCTTC GAAGAC(2/6)
BcgI 924 CGAGTGGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 4863 AGATCT A^GATCT
BpuAI 3658 GTCTTC GAAGAC(2/6)
BsaHI 870 GACGCC G[GA]^CG[CT]C
BspHI 1488 TCATGA T^CATGA
BspLU11I 5089 ACATGT A^CATGT
BsrFI 3040 ACCGGT [GA]^CCGG[CT]
Bst1107I 4230 GTATAC GTA^TAC
BstBI 2 TTCGAA TT^CGAA
BstEII 2303 GGTCACC G^GT[ACGT]ACC
BstPI 2303 GGTCACC G^GT[ACGT]ACC
BstZ17I 4230 GTATAC GTA^TAC
Cfr10I 3040 ACCGGT [GA]^CCGG[CT]
DraIII 817 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2517 CGGCCG C^GGCCG
EclXI 2517 CGGCCG C^GGCCG
Eco52I 2517 CGGCCG C^GGCCG
EcoO65I 2303 GGTCACC G^GT[ACGT]ACC
Hin1I 870 GACGCC G[GA]^CG[CT]C
HincII 4857 GTTGAC GT[CT]^[GA]AC
HindII 4857 GTTGAC GT[CT]^[GA]AC
NheI 3850 GCTAGC G^CTAGC
NspV 2 TTCGAA TT^CGAA
PflFI 2903 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3040 ACCGGT A^CCGGT
PmaCI 845 CACGTG CAC^GTG
PmlI 845 CACGTG CAC^GTG
PshBI 4132 ATTAAT AT^TAAT
PvuI 380 CGATCG CGAT^CG
RcaI 1488 TCATGA T^CATGA
SfuI 2 TTCGAA TT^CGAA
SmaI 1447 CCCGGG CCC^GGG
SphI 1086 GCATGC GCATG^C
SwaI 4219 ATTTAAAT ATTT^AAAT
Tth111I 2903 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3199 TCTAGA T^CTAGA
XmaCI 1447 CCCGGG C^CCGGG
XmaI 1447 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 4230 GTATAC GT^[AC][GT]AC
5212 GTAGAC
AflII 2820 CTTAAG C^TTAAG
5076 CTTAAG
AflIII 844 ACACGT A^C[GA][CT]GT
5089 ACATGT
Aor51HI 3802 AGCGCT AGC^GCT
5199 AGCGCT
BamHI 2595 GGATCC G^GATCC
4459 GGATCC
BanI 1226 GGTGCC G^G[CT][GA]CC
2604 GGTGCC
BbvCI 560 CCTCAGC CCTCAGC(-5/-2)
1234 CCTCAGC
BfrI 2820 CTTAAG C^TTAAG
5076 CTTAAG
BglI 2621 GCCACCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3617 GCCCAAAAGGC
BsaAI 845 CACGTG [CT]AC^GT[GA]
3225 CACGTA
BsiEI 380 CGATCG CG[GA][CT]^CG
2517 CGGCCG
BsmBI 1985 CGTCTC CGTCTC(1/5)
2513 GAGACG
BsmI 2709 GAATGC GAATGC(1/-1)
199 GCATTC
BsrBI 771 CCGCTC CCGCTC(-3/-3)
2334 CCGCTC
DrdI 3599 GACCCTCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4901 GACAGGTCTGTC
Eco47III 3802 AGCGCT AGC^GCT
5199 AGCGCT
EcoNI 796 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3530 CCTCCGCTAGG
EcoT22I 4065 ATGCAT ATGCA^T
4082 ATGCAT
FauI 177 CCCGC CCCGC(4/6)
754 CCCGC
HgaI 870 GACGC GACGC(5/10)
2110 GACGC
NcoI 155 CCATGG C^CATGG
2207 CCATGG
NsiI 4065 ATGCAT ATGCA^T
4082 ATGCAT
NspI 1086 GCATGC [GA]CATG^[CT]
5089 ACATGT
SacI 2325 GAGCTC GAGCT^C
2677 GAGCTC
SapI 327 GAAGAGC GCTCTTC(1/4)
505 GCTCTTC
ScaI 3483 AGTACT AGT^ACT
5072 AGTACT
TfiI 3489 GAATC G^A[AT]TC
4711 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI Asp700I AspHI
AvaI AvaII BalI BanII BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI