##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII AccIII AgeI AhdI Aor51HI
AscI AspEI AviII BcgI BseAI
BsiEI BsiWI BsmBI BspEI BsrDI
BssHII BstBI CpoI EagI Eam1105I
EclXI Eco47III Eco52I FseI FspI
KspI MfeI MluI MroI MunI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PvuI RsrII
SacII SapI SbfI SfiI SfuI
SgrAI SnaBI Sse8387I SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccII 4406 CGCG CG^CG
AclI 2336 AACGTT AA^CGTT
ApaI 5996 GGGCCC GGGCC^C
AseI 6633 ATTAAT AT^TAAT
AsnI 6633 ATTAAT AT^TAAT
Asp700I 2963 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 5589 CCTAGG C^CTAGG
BamHI 479 GGATCC G^GATCC
BbvCI 6314 CCTCAGC CCTCAGC(-5/-2)
BlnI 5589 CCTAGG C^CTAGG
BsaWI 3808 TCCGGT [AT]^CCGG[AT]
Bsp1407I 2475 TGTACA T^GTACA
BsrGI 2475 TGTACA T^GTACA
BstAPI 4353 GCAGATATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 4406 CGCG CG^CG
DraI 5716 TTTAAA TTT^AAA
DrdI 2025 GACCCACGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 3660 ATGCAT ATGCA^T
HpaI 903 GTTAAC GTT^AAC
MvnI 4406 CGCG CG^CG
NaeI 631 GCCGGC GCC^GGC
NgoMIV 631 GCCGGC G^CCGGC
NsiI 3660 ATGCAT ATGCA^T
PflMI 137 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1798 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 6633 ATTAAT AT^TAAT
Psp1406I 2336 AACGTT AA^CGTT
SalI 1977 GTCGAC G^TCGAC
ScaI 3461 AGTACT AGT^ACT
SexAI 3667 ACCAGGT A^CC[AT]GGT
SphI 4424 GCATGC GCATG^C
SspBI 2475 TGTACA T^GTACA
SspI 5056 AATATT AAT^ATT
Van91I 137 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2963 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 1677 GGTACC G^GTACC
1812 GGTACC
AcyI 92 GGCGCC G[GA]^CG[CT]C
3571 GGCGCC
AflII 1071 CTTAAG C^TTAAG
5528 CTTAAG
AflIII 511 ACATGT A^C[GA][CT]GT
4051 ACATGT
Alw44I 83 GTGCAC G^TGCAC
4014 GTGCAC
AocI 1287 CCTCAGG CC^T[ACGT]AGG
6048 CCTCAGG
ApaLI 83 GTGCAC G^TGCAC
4014 GTGCAC
Asp718I 1677 GGTACC G^GTACC
1812 GGTACC
AspI 1733 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3194 GACTGAGTC
AvaI 1987 CCCGGG C^[CT]CG[GA]G
3814 CCCGGG
BalI 1103 TGGCCA TGG^CCA
5944 TGGCCA
BbeI 92 GGCGCC GGCGC^C
3571 GGCGCC
BbrPI 2550 CACGTG CAC^GTG
2946 CACGTG
BciVI 4090 GTATCC GTATCC(6/5)
5343 GTATCC
BclI 3826 TGATCA T^GATCA
5292 TGATCA
BfrI 1071 CTTAAG C^TTAAG
5528 CTTAAG
BglI 1105 GCCAGCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
6397 GCCAAGAAGGC
BsaAI 2550 CACGTG [CT]AC^GT[GA]
2946 CACGTG
BsaBI 1034 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1220 GATGGACATC
BsaHI 92 GGCGCC G[GA]^CG[CT]C
3571 GGCGCC
BsoBI 1987 CCCGGG C^[CT]CG[GA]G
3814 CCCGGG
BspLU11I 511 ACATGT A^CATGT
4051 ACATGT
BsrBI 12 GAGCGG CCGCTC(-3/-3)
3845 GAGCGG
BsrFI 631 GCCGGC [GA]^CCGG[CT]
4767 GCCGGT
BssSI 1017 CTCGTG CACGAG(-5/-1)
1528 CTCGTG
Bsu36I 1287 CCTCAGG CC^T[ACGT]AGG
6048 CCTCAGG
Cfr10I 631 GCCGGC [GA]^CCGG[CT]
4767 GCCGGT
DraIII 1581 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
4804 CACAGTGTG
Eco81I 1287 CCTCAGG CC^T[ACGT]AGG
6048 CCTCAGG
EcoRI 855 GAATTC G^AATTC
1890 GAATTC
EcoRV 4314 GATATC GAT^ATC
5479 GATATC
FbaI 3826 TGATCA T^GATCA
5292 TGATCA
HgaI 3330 GACGC GACGC(5/10)
2587 GCGTC
Hin1I 92 GGCGCC G[GA]^CG[CT]C
3571 GGCGCC
HindIII 2779 AAGCTT A^AGCTT
6712 AAGCTT
KasI 92 GGCGCC G^GCGCC
3571 GGCGCC
KpnI 1677 GGTACC GGTAC^C
1812 GGTACC
MamI 1034 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1220 GATGGACATC
MluNI 1103 TGGCCA TGG^CCA
5944 TGGCCA
MscI 1103 TGGCCA TGG^CCA
5944 TGGCCA
NarI 92 GGCGCC GG^CGCC
3571 GGCGCC
NdeI 4471 CATATG CA^TATG
6178 CATATG
PflFI 1733 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3194 GACTGAGTC
PmaCI 2550 CACGTG CAC^GTG
2946 CACGTG
PmlI 2550 CACGTG CAC^GTG
2946 CACGTG
SfoI 92 GGCGCC GGC^GCC
3571 GGCGCC
SmaI 1987 CCCGGG CCC^GGG
3814 CCCGGG
SpeI 2064 ACTAGT A^CTAGT
5649 ACTAGT
Tth111I 1733 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3194 GACTGAGTC
XmaCI 1987 CCCGGG C^CCGGG
3814 CCCGGG
XmaI 1987 CCCGGG C^CCGGG
3814 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspDI BspHI
BspMI BsrI BssKI Bst1107I BstEII
BstF5I BstNI BstPI BstXI BstYI
BstZ17I Cac8I CelII CfoI Cfr13I
ClaI DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII