################################## # NO CUT ENZYMES ( 51 enzymes) # ################################## AatII AccIII AgeI AhdI Aor51HI AscI AspEI AviII BcgI BseAI BsiEI BsiWI BsmBI BspEI BsrDI BssHII BstBI CpoI EagI Eam1105I EclXI Eco47III Eco52I FseI FspI KspI MfeI MluI MroI MunI NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PvuI RsrII SacII SapI SbfI SfiI SfuI SgrAI SnaBI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccII 4406 CGCG CG^CG AclI 2336 AACGTT AA^CGTT ApaI 5996 GGGCCC GGGCC^C AseI 6633 ATTAAT AT^TAAT AsnI 6633 ATTAAT AT^TAAT Asp700I 2963 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 5589 CCTAGG C^CTAGG BamHI 479 GGATCC G^GATCC BbvCI 6314 CCTCAGC CCTCAGC(-5/-2) BlnI 5589 CCTAGG C^CTAGG BsaWI 3808 TCCGGT [AT]^CCGG[AT] Bsp1407I 2475 TGTACA T^GTACA BsrGI 2475 TGTACA T^GTACA BstAPI 4353 GCAGATATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 4406 CGCG CG^CG DraI 5716 TTTAAA TTT^AAA DrdI 2025 GACCCACGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT22I 3660 ATGCAT ATGCA^T HpaI 903 GTTAAC GTT^AAC MvnI 4406 CGCG CG^CG NaeI 631 GCCGGC GCC^GGC NgoMIV 631 GCCGGC G^CCGGC NsiI 3660 ATGCAT ATGCA^T PflMI 137 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1798 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 6633 ATTAAT AT^TAAT Psp1406I 2336 AACGTT AA^CGTT SalI 1977 GTCGAC G^TCGAC ScaI 3461 AGTACT AGT^ACT SexAI 3667 ACCAGGT A^CC[AT]GGT SphI 4424 GCATGC GCATG^C SspBI 2475 TGTACA T^GTACA SspI 5056 AATATT AAT^ATT Van91I 137 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 2963 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 1677 GGTACC G^GTACC 1812 GGTACC AcyI 92 GGCGCC G[GA]^CG[CT]C 3571 GGCGCC AflII 1071 CTTAAG C^TTAAG 5528 CTTAAG AflIII 511 ACATGT A^C[GA][CT]GT 4051 ACATGT Alw44I 83 GTGCAC G^TGCAC 4014 GTGCAC AocI 1287 CCTCAGG CC^T[ACGT]AGG 6048 CCTCAGG ApaLI 83 GTGCAC G^TGCAC 4014 GTGCAC Asp718I 1677 GGTACC G^GTACC 1812 GGTACC AspI 1733 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3194 GACTGAGTC AvaI 1987 CCCGGG C^[CT]CG[GA]G 3814 CCCGGG BalI 1103 TGGCCA TGG^CCA 5944 TGGCCA BbeI 92 GGCGCC GGCGC^C 3571 GGCGCC BbrPI 2550 CACGTG CAC^GTG 2946 CACGTG BciVI 4090 GTATCC GTATCC(6/5) 5343 GTATCC BclI 3826 TGATCA T^GATCA 5292 TGATCA BfrI 1071 CTTAAG C^TTAAG 5528 CTTAAG BglI 1105 GCCAGCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 6397 GCCAAGAAGGC BsaAI 2550 CACGTG [CT]AC^GT[GA] 2946 CACGTG BsaBI 1034 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1220 GATGGACATC BsaHI 92 GGCGCC G[GA]^CG[CT]C 3571 GGCGCC BsoBI 1987 CCCGGG C^[CT]CG[GA]G 3814 CCCGGG BspLU11I 511 ACATGT A^CATGT 4051 ACATGT BsrBI 12 GAGCGG CCGCTC(-3/-3) 3845 GAGCGG BsrFI 631 GCCGGC [GA]^CCGG[CT] 4767 GCCGGT BssSI 1017 CTCGTG CACGAG(-5/-1) 1528 CTCGTG Bsu36I 1287 CCTCAGG CC^T[ACGT]AGG 6048 CCTCAGG Cfr10I 631 GCCGGC [GA]^CCGG[CT] 4767 GCCGGT DraIII 1581 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG 4804 CACAGTGTG Eco81I 1287 CCTCAGG CC^T[ACGT]AGG 6048 CCTCAGG EcoRI 855 GAATTC G^AATTC 1890 GAATTC EcoRV 4314 GATATC GAT^ATC 5479 GATATC FbaI 3826 TGATCA T^GATCA 5292 TGATCA HgaI 3330 GACGC GACGC(5/10) 2587 GCGTC Hin1I 92 GGCGCC G[GA]^CG[CT]C 3571 GGCGCC HindIII 2779 AAGCTT A^AGCTT 6712 AAGCTT KasI 92 GGCGCC G^GCGCC 3571 GGCGCC KpnI 1677 GGTACC GGTAC^C 1812 GGTACC MamI 1034 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1220 GATGGACATC MluNI 1103 TGGCCA TGG^CCA 5944 TGGCCA MscI 1103 TGGCCA TGG^CCA 5944 TGGCCA NarI 92 GGCGCC GG^CGCC 3571 GGCGCC NdeI 4471 CATATG CA^TATG 6178 CATATG PflFI 1733 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3194 GACTGAGTC PmaCI 2550 CACGTG CAC^GTG 2946 CACGTG PmlI 2550 CACGTG CAC^GTG 2946 CACGTG SfoI 92 GGCGCC GGC^GCC 3571 GGCGCC SmaI 1987 CCCGGG CCC^GGG 3814 CCCGGG SpeI 2064 ACTAGT A^CTAGT 5649 ACTAGT Tth111I 1733 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3194 GACTGAGTC XmaCI 1987 CCCGGG C^CCGGG 3814 CCCGGG XmaI 1987 CCCGGG C^CCGGG 3814 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanI BanII BbsI BbvI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspDI BspHI BspMI BsrI BssKI Bst1107I BstEII BstF5I BstNI BstPI BstXI BstYI BstZ17I Cac8I CelII CfoI Cfr13I ClaI DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII