##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccII AccIII AclI AflIII
AgeI AscI AseI AsnI AvrII
BbeI BbrPI BlnI BsaI BseAI
BsiEI BsiWI BspDI BspEI BspLU11I
BspMI BsrFI BssHII Bst1107I BstBI
BstUI BstZ17I Cfr10I ClaI CpoI
EagI EclXI Eco52I FseI HpaI
KasI KspI MluI MroI MvnI
NaeI NarI NgoMIV NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfoI
SfuI SgrAI SpeI Sse8387I XcmI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 1460 GGTACC G^GTACC
AccI 166 GTCTAC GT^[AC][GT]AC
AhdI 2719 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 6731 GTGCAC G^TGCAC
AocI 5168 CCTGAGG CC^T[ACGT]AGG
Aor51HI 5037 AGCGCT AGC^GCT
ApaLI 6731 GTGCAC G^TGCAC
Asp718I 1460 GGTACC G^GTACC
AspEI 2719 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2120 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2256 TGCGCA TGC^GCA
BamHI 2917 GGATCC G^GATCC
BcgI 4791 GCAGCCTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 2012 GCCAGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2299 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2299 GCTTAGC GC^T[ACGT]AGC
BsaAI 6353 TACGTA [CT]AC^GT[GA]
BsaWI 2685 ACCGGA [AT]^CCGG[AT]
Bsp1407I 158 TGTACA T^GTACA
BsrGI 158 TGTACA T^GTACA
BstEII 5292 GGTCACC G^GT[ACGT]ACC
BstPI 5292 GGTCACC G^GT[ACGT]ACC
Bsu36I 5168 CCTGAGG CC^T[ACGT]AGG
CelII 2299 GCTTAGC GC^T[ACGT]AGC
DraIII 7 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2719 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5037 AGCGCT AGC^GCT
Eco81I 5168 CCTGAGG CC^T[ACGT]AGG
EcoO65I 5292 GGTCACC G^GT[ACGT]ACC
EcoRI 1912 GAATTC G^AATTC
EcoRV 712 GATATC GAT^ATC
EcoT22I 5227 ATGCAT ATGCA^T
FspI 2256 TGCGCA TGC^GCA
KpnI 1460 GGTACC GGTAC^C
MfeI 243 CAATTG C^AATTG
MunI 243 CAATTG C^AATTG
NheI 4802 GCTAGC G^CTAGC
NsiI 5227 ATGCAT ATGCA^T
PflFI 2120 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 1468 GAGCTC GAGCT^C
SexAI 3666 ACCAGGT A^CC[AT]GGT
SfiI 2011 GGCCAGGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 4297 CCCGGG CCC^GGG
SnaBI 6353 TACGTA TAC^GTA
SspBI 158 TGTACA T^GTACA
SwaI 5424 ATTTAAAT ATTT^AAAT
Tth111I 2120 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 4297 CCCGGG C^CCGGG
XmaI 4297 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflII 4349 CTTAAG C^TTAAG
5440 CTTAAG
ApaI 2146 GGGCCC GGGCC^C
4970 GGGCCC
BbvCI 3066 CCTCAGC CCTCAGC(-5/-2)
3308 GCTGAGG
BfrI 4349 CTTAAG C^TTAAG
5440 CTTAAG
BsaBI 337 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6434 GATGTCCATC
BsmBI 5337 CGTCTC CGTCTC(1/5)
2572 GAGACG
BsmI 1822 GAATGC GAATGC(1/-1)
5127 GCATTC
BspHI 1977 TCATGA T^CATGA
6533 TCATGA
BsrBI 2996 CCGCTC CCGCTC(-3/-3)
4720 GAGCGG
BssSI 2494 CACGAG CACGAG(-5/-1)
1245 CTCGTG
BstAPI 213 GCATCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6542 GCATATGTTGC
FauI 2995 CCCGC CCCGC(4/6)
187 GCGGG
HaeII 1087 AGCGCC [GA]GCGC^[CT]
5037 AGCGCT
MamI 337 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6434 GATGTCCATC
NdeI 533 CATATG CA^TATG
6543 CATATG
PflMI 6 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5253 CCAGGCACTGG
PshAI 1031 GACACTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2552 GACATCAGTC
RcaI 1977 TCATGA T^CATGA
6533 TCATGA
Van91I 6 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5253 CCAGGCACTGG
XbaI 2710 TCTAGA T^CTAGA
4114 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BciVI BclI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DrdI DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PaeR7I PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoI XhoII XmnI