##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII Acc65I AccIII AclI AocI
ApaI AscI Asp718I AspI BamHI
BbrPI BciVI BglI BsaAI BsaBI
BseAI BsiWI BsmBI BspEI BstBI
Bsu36I CpoI Eco81I EcoRI FseI
KpnI MamI MluI MroI NruI
NspV PacI PflFI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SalI SbfI SexAI SfiI SfuI
SmaI SnaBI SpeI Sse8387I Tth111I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AflII 4435 CTTAAG C^TTAAG
AgeI 4192 ACCGGT A^CCGGT
AhdI 3461 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 644 GTGCAC G^TGCAC
Aor51HI 140 AGCGCT AGC^GCT
ApaLI 644 GTGCAC G^TGCAC
AseI 6106 ATTAAT AT^TAAT
AsnI 6106 ATTAAT AT^TAAT
Asp700I 1559 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3461 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4912 TGCGCA TGC^GCA
BclI 4934 TGATCA T^GATCA
BfrI 4435 CTTAAG C^TTAAG
BsaI 3017 GAGACC GGTCTC(1/5)
BsaWI 4192 ACCGGT [AT]^CCGG[AT]
Bsp1407I 5179 TGTACA T^GTACA
BspDI 2851 ATCGAT AT^CGAT
BspMI 274 ACCTGC ACCTGC(4/8)
BsrGI 5179 TGTACA T^GTACA
Bst1107I 6175 GTATAC GTA^TAC
BstEII 229 GGTCACC G^GT[ACGT]ACC
BstPI 229 GGTCACC G^GT[ACGT]ACC
BstXI 5389 CCACAACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 6175 GTATAC GTA^TAC
ClaI 2851 ATCGAT AT^CGAT
DraIII 1290 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4074 GACAGTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3461 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 140 AGCGCT AGC^GCT
EcoNI 1682 CCTACCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 229 GGTCACC G^GT[ACGT]ACC
EcoT22I 662 ATGCAT ATGCA^T
FbaI 4934 TGATCA T^GATCA
FspI 4912 TGCGCA TGC^GCA
KspI 148 CCGCGG CCGC^GG
MaeII 381 ACGT A^CGT
NaeI 193 GCCGGC GCC^GGC
NgoMIV 193 GCCGGC G^CCGGC
NotI 2814 GCGGCCGC GC^GGCCGC
NsiI 662 ATGCAT ATGCA^T
PaeR7I 3647 CTCGAG C^TCGAG
PinAI 4192 ACCGGT A^CCGGT
PshBI 6106 ATTAAT AT^TAAT
PvuII 3270 CAGCTG CAG^CTG
SacII 148 CCGCGG CCGC^GG
SapI 2871 GCTCTTC GCTCTTC(1/4)
SgrAI 192 CGCCGGCG C[GA]^CCGG[CT]G
SphI 2555 GCATGC GCATG^C
SspBI 5179 TGTACA T^GTACA
SwaI 4412 ATTTAAAT ATTT^AAAT
XbaI 5530 TCTAGA T^CTAGA
XhoI 3647 CTCGAG C^TCGAG
XmnI 1559 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AvrII 3049 CCTAGG C^CTAGG
5192 CCTAGG
BcgI 2853 CGATGAGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2719 GCAGCGCCGTCG
BglII 280 AGATCT A^GATCT
6363 AGATCT
BlnI 3049 CCTAGG C^CTAGG
5192 CCTAGG
BsiEI 312 CGGCCG CG[GA][CT]^CG
2815 CGGCCG
BsrDI 1761 CATTGC GCAATG(2/0)
5020 GCAATG
BssHII 73 GCGCGC G^CGCGC
161 GCGCGC
EagI 312 CGGCCG C^GGCCG
2815 CGGCCG
EclXI 312 CGGCCG C^GGCCG
2815 CGGCCG
Eco52I 312 CGGCCG C^GGCCG
2815 CGGCCG
HindIII 1227 AAGCTT A^AGCTT
3995 AAGCTT
HpaI 1951 GTTAAC GTT^AAC
5434 GTTAAC
NheI 4360 GCTAGC G^CTAGC
5088 GCTAGC
ScaI 350 AGTACT AGT^ACT
3968 AGTACT
XcmI 2280 CCAAAGAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2958 CCAACATACTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspHI BspLU11I BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoRV EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeIII MboI MboII
MfeI MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MunI
MvaI MvnI MwoI NarI NciI
NcoI NdeI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII