##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        AhdI        Aor51HI     AscI        
Asp718I     AspEI       AviII       AvrII       BbeI        
BbrPI       BfrI        BglII       BlnI        BsaHI       
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BspDI       BspEI       BsrBI       BssSI       BstBI       
BstEII      BstPI       ClaI        CpoI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO65I     
EcoRV       FseI        FspI        HaeII       Hin1I       
KasI        KpnI        KspI        MfeI        MluI        
MroI        MunI        NarI        NdeI        NotI        
NruI        NspV        PacI        PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    StuI        SwaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

ApaI         4601  GGGCCC               GGGCC^C
AseI         2360  ATTAAT               AT^TAAT
AsnI         2360  ATTAAT               AT^TAAT
Asp700I      1667  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         3657  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        5425  GGATCC               G^GATCC
BcgI         5459  GCAGGTGGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        5021  GGATAC               GTATCC(6/5)
BglI         1302  GCCTGCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        1646  TACGTG               [CT]AC^GT[GA]
BsaBI        3823  GATGCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI          658  GCATTC               GAATGC(1/-1)
BspHI        1723  TCATGA               T^CATGA
BsrDI        5569  CATTGC               GCAATG(2/0)
BsrFI        2485  GCCGGC               [GA]^CCGG[CT]
BstXI        3803  CCAAGAATTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I       2485  GCCGGC               [GA]^CCGG[CT]
EcoT22I       963  ATGCAT               ATGCA^T
HgaI          784  GACGC                GACGC(5/10)
HpaI         2349  GTTAAC               GTT^AAC
MamI         3823  GATGCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI         2485  GCCGGC               GCC^GGC
NgoMIV       2485  GCCGGC               G^CCGGC
NheI         4772  GCTAGC               G^CTAGC
NsiI          963  ATGCAT               ATGCA^T
PaeR7I       2298  CTCGAG               C^TCGAG
PflFI        3657  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI        2360  ATTAAT               AT^TAAT
RcaI         1723  TCATGA               T^CATGA
SexAI        5833  ACCAGGT              A^CC[AT]GGT
SmaI         4588  CCCGGG               CCC^GGG
SspI         4892  AATATT               AAT^ATT
Tth111I      3657  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         2298  CTCGAG               C^TCGAG
XmaCI        4588  CCCGGG               C^CCGGG
XmaI         4588  CCCGGG               C^CCGGG
XmnI         1667  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AflIII       1957  ACATGT               A^C[GA][CT]GT
             5100  ACATGT              
Alw44I       2105  GTGCAC               G^TGCAC
             2624  GTGCAC              
ApaLI        2105  GTGCAC               G^TGCAC
             2624  GTGCAC              
BbsI          811  GAAGAC               GAAGAC(2/6)
             1811  GTCTTC              
BclI          714  TGATCA               T^GATCA
             2757  TGATCA              
BpuAI         811  GAAGAC               GAAGAC(2/6)
             1811  GTCTTC              
Bsp1407I      530  TGTACA               T^GTACA
             2179  TGTACA              
BspLU11I     1957  ACATGT               A^CATGT
             5100  ACATGT              
BsrGI         530  TGTACA               T^GTACA
             2179  TGTACA              
BssHII       2097  GCGCGC               G^CGCGC
             2099  GCGCGC              
Bst1107I       31  GTATAC               GTA^TAC
              427  GTATAC              
BstAPI       5189  GCACACGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             5519  GCATGCTTTGC         
BstZ17I        31  GTATAC               GTA^TAC
              427  GTATAC              
DrdI          784  GACGCAGGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4287  GACCTACAAGTC        
FbaI          714  TGATCA               T^GATCA
             2757  TGATCA              
HindIII      2053  AAGCTT               A^AGCTT
             2188  AAGCTT              
PflMI        3504  CCAGCTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4529  CCAGCTGGTGG         
SapI          743  GCTCTTC              GCTCTTC(1/4)
             5258  GCTCTTC             
ScaI         1234  AGTACT               AGT^ACT
             2514  AGTACT              
SspBI         530  TGTACA               T^GTACA
             2179  TGTACA              
Van91I       3504  CCAGCTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4529  CCAGCTGGTGG         
XbaI         3207  TCTAGA               T^CTAGA
             5802  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  121 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AluI        AlwI        AlwNI       AocI        ApoI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbvCI       BbvI        BcnI        BfaI        
BlpI        BmyI        BpmI        Bpu1102I    BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Bsu36I      Cac8I       CelII       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraII       DraIII      DsaI        EaeI        EarI        
Eco81I      EcoNI       EcoO109I    EcoRI       EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PpuMI       PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SphI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoII