################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI Aor51HI AscI Asp718I AspEI AviII AvrII BbeI BbrPI BfrI BglII BlnI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrBI BssSI BstBI BstEII BstPI ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI HaeII Hin1I KasI KpnI KspI MfeI MluI MroI MunI NarI NdeI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I StuI SwaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### ApaI 4601 GGGCCC GGGCC^C AseI 2360 ATTAAT AT^TAAT AsnI 2360 ATTAAT AT^TAAT Asp700I 1667 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 3657 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 5425 GGATCC G^GATCC BcgI 5459 GCAGGTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 5021 GGATAC GTATCC(6/5) BglI 1302 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 1646 TACGTG [CT]AC^GT[GA] BsaBI 3823 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmI 658 GCATTC GAATGC(1/-1) BspHI 1723 TCATGA T^CATGA BsrDI 5569 CATTGC GCAATG(2/0) BsrFI 2485 GCCGGC [GA]^CCGG[CT] BstXI 3803 CCAAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 2485 GCCGGC [GA]^CCGG[CT] EcoT22I 963 ATGCAT ATGCA^T HgaI 784 GACGC GACGC(5/10) HpaI 2349 GTTAAC GTT^AAC MamI 3823 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 2485 GCCGGC GCC^GGC NgoMIV 2485 GCCGGC G^CCGGC NheI 4772 GCTAGC G^CTAGC NsiI 963 ATGCAT ATGCA^T PaeR7I 2298 CTCGAG C^TCGAG PflFI 3657 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 2360 ATTAAT AT^TAAT RcaI 1723 TCATGA T^CATGA SexAI 5833 ACCAGGT A^CC[AT]GGT SmaI 4588 CCCGGG CCC^GGG SspI 4892 AATATT AAT^ATT Tth111I 3657 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2298 CTCGAG C^TCGAG XmaCI 4588 CCCGGG C^CCGGG XmaI 4588 CCCGGG C^CCGGG XmnI 1667 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AflIII 1957 ACATGT A^C[GA][CT]GT 5100 ACATGT Alw44I 2105 GTGCAC G^TGCAC 2624 GTGCAC ApaLI 2105 GTGCAC G^TGCAC 2624 GTGCAC BbsI 811 GAAGAC GAAGAC(2/6) 1811 GTCTTC BclI 714 TGATCA T^GATCA 2757 TGATCA BpuAI 811 GAAGAC GAAGAC(2/6) 1811 GTCTTC Bsp1407I 530 TGTACA T^GTACA 2179 TGTACA BspLU11I 1957 ACATGT A^CATGT 5100 ACATGT BsrGI 530 TGTACA T^GTACA 2179 TGTACA BssHII 2097 GCGCGC G^CGCGC 2099 GCGCGC Bst1107I 31 GTATAC GTA^TAC 427 GTATAC BstAPI 5189 GCACACGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5519 GCATGCTTTGC BstZ17I 31 GTATAC GTA^TAC 427 GTATAC DrdI 784 GACGCAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4287 GACCTACAAGTC FbaI 714 TGATCA T^GATCA 2757 TGATCA HindIII 2053 AAGCTT A^AGCTT 2188 AAGCTT PflMI 3504 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4529 CCAGCTGGTGG SapI 743 GCTCTTC GCTCTTC(1/4) 5258 GCTCTTC ScaI 1234 AGTACT AGT^ACT 2514 AGTACT SspBI 530 TGTACA T^GTACA 2179 TGTACA Van91I 3504 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4529 CCAGCTGGTGG XbaI 3207 TCTAGA T^CTAGA 5802 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII