##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Aor51HI AscI
Asp718I AspEI AviII AvrII BbeI
BbrPI BfrI BglII BlnI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BsrBI BssSI BstBI
BstEII BstPI ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV FseI FspI HaeII Hin1I
KasI KpnI KspI MfeI MluI
MroI MunI NarI NdeI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I StuI SwaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
ApaI 4601 GGGCCC GGGCC^C
AseI 2360 ATTAAT AT^TAAT
AsnI 2360 ATTAAT AT^TAAT
Asp700I 1667 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3657 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 5425 GGATCC G^GATCC
BcgI 5459 GCAGGTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5021 GGATAC GTATCC(6/5)
BglI 1302 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1646 TACGTG [CT]AC^GT[GA]
BsaBI 3823 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 658 GCATTC GAATGC(1/-1)
BspHI 1723 TCATGA T^CATGA
BsrDI 5569 CATTGC GCAATG(2/0)
BsrFI 2485 GCCGGC [GA]^CCGG[CT]
BstXI 3803 CCAAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 2485 GCCGGC [GA]^CCGG[CT]
EcoT22I 963 ATGCAT ATGCA^T
HgaI 784 GACGC GACGC(5/10)
HpaI 2349 GTTAAC GTT^AAC
MamI 3823 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2485 GCCGGC GCC^GGC
NgoMIV 2485 GCCGGC G^CCGGC
NheI 4772 GCTAGC G^CTAGC
NsiI 963 ATGCAT ATGCA^T
PaeR7I 2298 CTCGAG C^TCGAG
PflFI 3657 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2360 ATTAAT AT^TAAT
RcaI 1723 TCATGA T^CATGA
SexAI 5833 ACCAGGT A^CC[AT]GGT
SmaI 4588 CCCGGG CCC^GGG
SspI 4892 AATATT AAT^ATT
Tth111I 3657 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2298 CTCGAG C^TCGAG
XmaCI 4588 CCCGGG C^CCGGG
XmaI 4588 CCCGGG C^CCGGG
XmnI 1667 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 1957 ACATGT A^C[GA][CT]GT
5100 ACATGT
Alw44I 2105 GTGCAC G^TGCAC
2624 GTGCAC
ApaLI 2105 GTGCAC G^TGCAC
2624 GTGCAC
BbsI 811 GAAGAC GAAGAC(2/6)
1811 GTCTTC
BclI 714 TGATCA T^GATCA
2757 TGATCA
BpuAI 811 GAAGAC GAAGAC(2/6)
1811 GTCTTC
Bsp1407I 530 TGTACA T^GTACA
2179 TGTACA
BspLU11I 1957 ACATGT A^CATGT
5100 ACATGT
BsrGI 530 TGTACA T^GTACA
2179 TGTACA
BssHII 2097 GCGCGC G^CGCGC
2099 GCGCGC
Bst1107I 31 GTATAC GTA^TAC
427 GTATAC
BstAPI 5189 GCACACGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5519 GCATGCTTTGC
BstZ17I 31 GTATAC GTA^TAC
427 GTATAC
DrdI 784 GACGCAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4287 GACCTACAAGTC
FbaI 714 TGATCA T^GATCA
2757 TGATCA
HindIII 2053 AAGCTT A^AGCTT
2188 AAGCTT
PflMI 3504 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4529 CCAGCTGGTGG
SapI 743 GCTCTTC GCTCTTC(1/4)
5258 GCTCTTC
ScaI 1234 AGTACT AGT^ACT
2514 AGTACT
SspBI 530 TGTACA T^GTACA
2179 TGTACA
Van91I 3504 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4529 CCAGCTGGTGG
XbaI 3207 TCTAGA T^CTAGA
5802 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII