##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AccII AclI AflII AgeI Aor51HI
AscI BbeI BclI BfrI BsiWI
BsmBI BssHII BstBI BstUI DraIII
Eco47III EcoRV FbaI FseI KasI
KspI MfeI MluI MunI MvnI
NarI NheI NotI NruI NspV
PacI PinAI PmeI Psp1406I PvuI
SacII SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
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# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccIII 2306 TCCGGA T^CCGGA
AseI 1840 ATTAAT AT^TAAT
AsnI 1840 ATTAAT AT^TAAT
AviII 5667 TGCGCA TGC^GCA
AvrII 1488 CCTAGG C^CTAGG
BbrPI 5879 CACGTG CAC^GTG
BcgI 4184 GCATTGGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1488 CCTAGG C^CTAGG
BsaAI 5879 CACGTG [CT]AC^GT[GA]
BsaBI 5085 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2306 TCCGGA [AT]^CCGG[AT]
BseAI 2306 TCCGGA T^CCGGA
Bsp1407I 1889 TGTACA T^GTACA
BspDI 1399 ATCGAT AT^CGAT
BspEI 2306 TCCGGA T^CCGGA
BspHI 228 TCATGA T^CATGA
BspLU11I 3913 ACATGT A^CATGT
BsrBI 474 GAGCGG CCGCTC(-3/-3)
BsrGI 1889 TGTACA T^GTACA
ClaI 1399 ATCGAT AT^CGAT
CpoI 1472 CGGTCCG CG^G[AT]CCG
DraI 6293 TTTAAA TTT^AAA
EagI 4327 CGGCCG C^GGCCG
EclXI 4327 CGGCCG C^GGCCG
Eco52I 4327 CGGCCG C^GGCCG
FspI 5667 TGCGCA TGC^GCA
HaeII 2061 GGCGCT [GA]GCGC^[CT]
HpaI 3667 GTTAAC GTT^AAC
MamI 5085 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2306 TCCGGA T^CCGGA
NaeI 4237 GCCGGC GCC^GGC
NdeI 5524 CATATG CA^TATG
NgoMIV 4237 GCCGGC G^CCGGC
PaeR7I 4889 CTCGAG C^TCGAG
PmaCI 5879 CACGTG CAC^GTG
PmlI 5879 CACGTG CAC^GTG
PshAI 354 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1840 ATTAAT AT^TAAT
RcaI 228 TCATGA T^CATGA
RsrII 1472 CGGTCCG CG^G[AT]CCG
ScaI 1772 AGTACT AGT^ACT
SexAI 4411 ACCTGGT A^CC[AT]GGT
SspBI 1889 TGTACA T^GTACA
SspI 6273 AATATT AAT^ATT
StuI 295 AGGCCT AGG^CCT
XbaI 6196 TCTAGA T^CTAGA
XhoI 4889 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AatII 982 GACGTC GACGT^C
6216 GACGTC
Alw44I 5207 GTGCAC G^TGCAC
6323 GTGCAC
ApaLI 5207 GTGCAC G^TGCAC
6323 GTGCAC
Asp700I 16 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3431 GAAACCTTTC
BciVI 234 GGATAC GTATCC(6/5)
3820 GGATAC
BglI 4347 GCCTCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5356 GCCCAGTAGGC
BsiEI 2583 CGACCG CG[GA][CT]^CG
4327 CGGCCG
BsrFI 4237 GCCGGC [GA]^CCGG[CT]
5640 GCCGGT
BstAPI 55 GCAGTAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3471 GCACTCCATGC
BstEII 738 GGTCACC G^GT[ACGT]ACC
1669 GGTTACC
BstPI 738 GGTCACC G^GT[ACGT]ACC
1669 GGTTACC
Cfr10I 4237 GCCGGC [GA]^CCGG[CT]
5640 GCCGGT
DrdI 145 GACACAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1336 GACCAGGGTGTC
EcoO65I 738 GGTCACC G^GT[ACGT]ACC
1669 GGTTACC
EcoT22I 5371 ATGCAT ATGCA^T
5375 ATGCAT
HgaI 4538 GACGC GACGC(5/10)
871 GCGTC
NsiI 5371 ATGCAT ATGCA^T
5375 ATGCAT
SmaI 1325 CCCGGG CCC^GGG
4691 CCCGGG
SphI 4811 GCATGC GCATG^C
5373 GCATGC
XmaCI 1325 CCCGGG C^CCGGG
4691 CCCGGG
XmaI 1325 CCCGGG C^CCGGG
4691 CCCGGG
XmnI 16 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3431 GAAACCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
Acc65I AccI AciI AcsI AcyI
AfaI AflIII AhdI AluI AlwI
AlwNI AocI ApaI ApoI Asp718I
AspEI AspHI AspI AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BssSI Bst1107I BstF5I
BstNI BstXI BstYI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI Eam1105I
EarI Eco81I EcoNI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI SalI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII