################################## # NO CUT ENZYMES ( 45 enzymes) # ################################## AccII AclI AflII AgeI Aor51HI AscI BbeI BclI BfrI BsiWI BsmBI BssHII BstBI BstUI DraIII Eco47III EcoRV FbaI FseI KasI KspI MfeI MluI MunI MvnI NarI NheI NotI NruI NspV PacI PinAI PmeI Psp1406I PvuI SacII SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccIII 2306 TCCGGA T^CCGGA AseI 1840 ATTAAT AT^TAAT AsnI 1840 ATTAAT AT^TAAT AviII 5667 TGCGCA TGC^GCA AvrII 1488 CCTAGG C^CTAGG BbrPI 5879 CACGTG CAC^GTG BcgI 4184 GCATTGGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 1488 CCTAGG C^CTAGG BsaAI 5879 CACGTG [CT]AC^GT[GA] BsaBI 5085 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2306 TCCGGA [AT]^CCGG[AT] BseAI 2306 TCCGGA T^CCGGA Bsp1407I 1889 TGTACA T^GTACA BspDI 1399 ATCGAT AT^CGAT BspEI 2306 TCCGGA T^CCGGA BspHI 228 TCATGA T^CATGA BspLU11I 3913 ACATGT A^CATGT BsrBI 474 GAGCGG CCGCTC(-3/-3) BsrGI 1889 TGTACA T^GTACA ClaI 1399 ATCGAT AT^CGAT CpoI 1472 CGGTCCG CG^G[AT]CCG DraI 6293 TTTAAA TTT^AAA EagI 4327 CGGCCG C^GGCCG EclXI 4327 CGGCCG C^GGCCG Eco52I 4327 CGGCCG C^GGCCG FspI 5667 TGCGCA TGC^GCA HaeII 2061 GGCGCT [GA]GCGC^[CT] HpaI 3667 GTTAAC GTT^AAC MamI 5085 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2306 TCCGGA T^CCGGA NaeI 4237 GCCGGC GCC^GGC NdeI 5524 CATATG CA^TATG NgoMIV 4237 GCCGGC G^CCGGC PaeR7I 4889 CTCGAG C^TCGAG PmaCI 5879 CACGTG CAC^GTG PmlI 5879 CACGTG CAC^GTG PshAI 354 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1840 ATTAAT AT^TAAT RcaI 228 TCATGA T^CATGA RsrII 1472 CGGTCCG CG^G[AT]CCG ScaI 1772 AGTACT AGT^ACT SexAI 4411 ACCTGGT A^CC[AT]GGT SspBI 1889 TGTACA T^GTACA SspI 6273 AATATT AAT^ATT StuI 295 AGGCCT AGG^CCT XbaI 6196 TCTAGA T^CTAGA XhoI 4889 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AatII 982 GACGTC GACGT^C 6216 GACGTC Alw44I 5207 GTGCAC G^TGCAC 6323 GTGCAC ApaLI 5207 GTGCAC G^TGCAC 6323 GTGCAC Asp700I 16 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3431 GAAACCTTTC BciVI 234 GGATAC GTATCC(6/5) 3820 GGATAC BglI 4347 GCCTCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 5356 GCCCAGTAGGC BsiEI 2583 CGACCG CG[GA][CT]^CG 4327 CGGCCG BsrFI 4237 GCCGGC [GA]^CCGG[CT] 5640 GCCGGT BstAPI 55 GCAGTAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3471 GCACTCCATGC BstEII 738 GGTCACC G^GT[ACGT]ACC 1669 GGTTACC BstPI 738 GGTCACC G^GT[ACGT]ACC 1669 GGTTACC Cfr10I 4237 GCCGGC [GA]^CCGG[CT] 5640 GCCGGT DrdI 145 GACACAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1336 GACCAGGGTGTC EcoO65I 738 GGTCACC G^GT[ACGT]ACC 1669 GGTTACC EcoT22I 5371 ATGCAT ATGCA^T 5375 ATGCAT HgaI 4538 GACGC GACGC(5/10) 871 GCGTC NsiI 5371 ATGCAT ATGCA^T 5375 ATGCAT SmaI 1325 CCCGGG CCC^GGG 4691 CCCGGG SphI 4811 GCATGC GCATG^C 5373 GCATGC XmaCI 1325 CCCGGG C^CCGGG 4691 CCCGGG XmaI 1325 CCCGGG C^CCGGG 4691 CCCGGG XmnI 16 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3431 GAAACCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 144 enzymes) # ######################################### Acc65I AccI AciI AcsI AcyI AfaI AflIII AhdI AluI AlwI AlwNI AocI ApaI ApoI Asp718I AspEI AspHI AspI AvaI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BssSI Bst1107I BstF5I BstNI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SacI SalI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII