################################## # NO CUT ENZYMES ( 38 enzymes) # ################################## AccIII AclI AflII AscI AseI AsnI BamHI BfrI BseAI BsiWI BsmBI BspDI BspEI BssHII BstEII BstPI ClaI CpoI EcoO65I EcoRV FseI KspI MluI MroI NotI NruI PacI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SfiI SnaBI SspI SwaI ################################### # ONE CUT ENZYMES ( 66 enzymes) # ################################### AatII 3228 GACGTC GACGT^C Acc65I 1440 GGTACC G^GTACC AcsI 5908 GAATTC [GA]^AATT[CT] AgeI 1167 ACCGGT A^CCGGT AhdI 5193 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1212 GTGCAC G^TGCAC Aor51HI 1361 AGCGCT AGC^GCT ApaLI 1212 GTGCAC G^TGCAC ApoI 5908 GAATTC [GA]^AATT[CT] Asp700I 2606 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1440 GGTACC G^GTACC AspEI 5193 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 477 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1912 TGCGCA TGC^GCA AvrII 6574 CCTAGG C^CTAGG BbrPI 1215 CACGTG CAC^GTG BbvCI 3371 CCTCAGC CCTCAGC(-5/-2) BcgI 4148 GCAGCGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 3439 TGATCA T^GATCA BlnI 6574 CCTAGG C^CTAGG BsaAI 1215 CACGTG [CT]AC^GT[GA] BsaBI 1448 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC Bst1107I 65 GTATAC GTA^TAC BstBI 4156 TTCGAA TT^CGAA BstZ17I 65 GTATAC GTA^TAC DraI 5582 TTTAAA TTT^AAA EagI 3156 CGGCCG C^GGCCG Eam1105I 5193 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 3156 CGGCCG C^GGCCG Eco47III 1361 AGCGCT AGC^GCT Eco52I 3156 CGGCCG C^GGCCG EcoRI 5908 GAATTC G^AATTC EcoT22I 1068 ATGCAT ATGCA^T FbaI 3439 TGATCA T^GATCA FspI 1912 TGCGCA TGC^GCA HindIII 2407 AAGCTT A^AGCTT HpaI 209 GTTAAC GTT^AAC KpnI 1440 GGTACC GGTAC^C MamI 1448 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3740 CAATTG C^AATTG MunI 3740 CAATTG C^AATTG NaeI 2243 GCCGGC GCC^GGC NdeI 2491 CATATG CA^TATG NgoMIV 2243 GCCGGC G^CCGGC NheI 5512 GCTAGC G^CTAGC NsiI 1068 ATGCAT ATGCA^T NspV 4156 TTCGAA TT^CGAA PaeR7I 2394 CTCGAG C^TCGAG PflFI 477 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 416 CCAGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1167 ACCGGT A^CCGGT PmaCI 1215 CACGTG CAC^GTG PmlI 1215 CACGTG CAC^GTG PshAI 3098 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SbfI 2702 CCTGCAGG CCTGCA^GG ScaI 7218 AGTACT AGT^ACT SexAI 3478 ACCTGGT A^CC[AT]GGT SfuI 4156 TTCGAA TT^CGAA SgrAI 1166 CACCGGTG C[GA]^CCGG[CT]G SpeI 3311 ACTAGT A^CTAGT Sse8387I 2702 CCTGCAGG CCTGCA^GG Tth111I 477 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 416 CCAGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 6456 TCTAGA T^CTAGA XhoI 2394 CTCGAG C^TCGAG XmnI 2606 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccI 65 GTATAC GT^[AC][GT]AC 7110 GTAGAC AocI 1664 CCTGAGG CC^T[ACGT]AGG 4868 CCTCAGG BciVI 2104 GTATCC GTATCC(6/5) 6478 GTATCC BglII 3544 AGATCT A^GATCT 4075 AGATCT BsaWI 1167 ACCGGT [AT]^CCGG[AT] 1186 TCCGGT BsmI 1472 GAATGC GAATGC(1/-1) 3053 GAATGC BspHI 2692 TCATGA T^CATGA 5882 TCATGA BsrFI 1167 ACCGGT [GA]^CCGG[CT] 2243 GCCGGC Bsu36I 1664 CCTGAGG CC^T[ACGT]AGG 4868 CCTCAGG Cfr10I 1167 ACCGGT [GA]^CCGG[CT] 2243 GCCGGC DrdI 3222 GACGAGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3290 GACGGATGGGTC Eco81I 1664 CCTGAGG CC^T[ACGT]AGG 4868 CCTCAGG EcoNI 1319 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4540 CCTCCAGAAGG HgaI 35 GACGC GACGC(5/10) 2946 GACGC RcaI 2692 TCATGA T^CATGA 5882 TCATGA SacI 4888 GAGCTC GAGCT^C 4972 GAGCTC SapI 165 GAAGAGC GCTCTTC(1/4) 3734 GAAGAGC SphI 1070 GCATGC GCATG^C 3388 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 136 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbeI BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI