##################################
# NO CUT ENZYMES (   38 enzymes) #
##################################

AccIII      AclI        AflII       AscI        AseI        
AsnI        BamHI       BfrI        BseAI       BsiWI       
BsmBI       BspDI       BspEI       BssHII      BstEII      
BstPI       ClaI        CpoI        EcoO65I     EcoRV       
FseI        KspI        MluI        MroI        NotI        
NruI        PacI        PmeI        PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SfiI        
SnaBI       SspI        SwaI        


###################################
# ONE CUT ENZYMES (   66 enzymes) #
###################################

AatII        3228  GACGTC               GACGT^C
Acc65I       1440  GGTACC               G^GTACC
AcsI         5908  GAATTC               [GA]^AATT[CT]
AgeI         1167  ACCGGT               A^CCGGT
AhdI         5193  GACTGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1212  GTGCAC               G^TGCAC
Aor51HI      1361  AGCGCT               AGC^GCT
ApaLI        1212  GTGCAC               G^TGCAC
ApoI         5908  GAATTC               [GA]^AATT[CT]
Asp700I      2606  GAAGCATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1440  GGTACC               G^GTACC
AspEI        5193  GACTGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          477  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1912  TGCGCA               TGC^GCA
AvrII        6574  CCTAGG               C^CTAGG
BbrPI        1215  CACGTG               CAC^GTG
BbvCI        3371  CCTCAGC              CCTCAGC(-5/-2)
BcgI         4148  GCAGCGGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         3439  TGATCA               T^GATCA
BlnI         6574  CCTAGG               C^CTAGG
BsaAI        1215  CACGTG               [CT]AC^GT[GA]
BsaBI        1448  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bst1107I       65  GTATAC               GTA^TAC
BstBI        4156  TTCGAA               TT^CGAA
BstZ17I        65  GTATAC               GTA^TAC
DraI         5582  TTTAAA               TTT^AAA
EagI         3156  CGGCCG               C^GGCCG
Eam1105I     5193  GACTGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        3156  CGGCCG               C^GGCCG
Eco47III     1361  AGCGCT               AGC^GCT
Eco52I       3156  CGGCCG               C^GGCCG
EcoRI        5908  GAATTC               G^AATTC
EcoT22I      1068  ATGCAT               ATGCA^T
FbaI         3439  TGATCA               T^GATCA
FspI         1912  TGCGCA               TGC^GCA
HindIII      2407  AAGCTT               A^AGCTT
HpaI          209  GTTAAC               GTT^AAC
KpnI         1440  GGTACC               GGTAC^C
MamI         1448  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         3740  CAATTG               C^AATTG
MunI         3740  CAATTG               C^AATTG
NaeI         2243  GCCGGC               GCC^GGC
NdeI         2491  CATATG               CA^TATG
NgoMIV       2243  GCCGGC               G^CCGGC
NheI         5512  GCTAGC               G^CTAGC
NsiI         1068  ATGCAT               ATGCA^T
NspV         4156  TTCGAA               TT^CGAA
PaeR7I       2394  CTCGAG               C^TCGAG
PflFI         477  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         416  CCAGCGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1167  ACCGGT               A^CCGGT
PmaCI        1215  CACGTG               CAC^GTG
PmlI         1215  CACGTG               CAC^GTG
PshAI        3098  GACCCAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI         2702  CCTGCAGG             CCTGCA^GG
ScaI         7218  AGTACT               AGT^ACT
SexAI        3478  ACCTGGT              A^CC[AT]GGT
SfuI         4156  TTCGAA               TT^CGAA
SgrAI        1166  CACCGGTG             C[GA]^CCGG[CT]G
SpeI         3311  ACTAGT               A^CTAGT
Sse8387I     2702  CCTGCAGG             CCTGCA^GG
Tth111I       477  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        416  CCAGCGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         6456  TCTAGA               T^CTAGA
XhoI         2394  CTCGAG               C^TCGAG
XmnI         2606  GAAGCATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AccI           65  GTATAC               GT^[AC][GT]AC
             7110  GTAGAC              
AocI         1664  CCTGAGG              CC^T[ACGT]AGG
             4868  CCTCAGG             
BciVI        2104  GTATCC               GTATCC(6/5)
             6478  GTATCC              
BglII        3544  AGATCT               A^GATCT
             4075  AGATCT              
BsaWI        1167  ACCGGT               [AT]^CCGG[AT]
             1186  TCCGGT              
BsmI         1472  GAATGC               GAATGC(1/-1)
             3053  GAATGC              
BspHI        2692  TCATGA               T^CATGA
             5882  TCATGA              
BsrFI        1167  ACCGGT               [GA]^CCGG[CT]
             2243  GCCGGC              
Bsu36I       1664  CCTGAGG              CC^T[ACGT]AGG
             4868  CCTCAGG             
Cfr10I       1167  ACCGGT               [GA]^CCGG[CT]
             2243  GCCGGC              
DrdI         3222  GACGAGGACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3290  GACGGATGGGTC        
Eco81I       1664  CCTGAGG              CC^T[ACGT]AGG
             4868  CCTCAGG             
EcoNI        1319  CCTGGTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4540  CCTCCAGAAGG         
HgaI           35  GACGC                GACGC(5/10)
             2946  GACGC               
RcaI         2692  TCATGA               T^CATGA
             5882  TCATGA              
SacI         4888  GAGCTC               GAGCT^C
             4972  GAGCTC              
SapI          165  GAAGAGC              GCTCTTC(1/4)
             3734  GAAGAGC             
SphI         1070  GCATGC               GCATG^C
             3388  GCATGC              


#########################################
# 3 OR MORE CUT ENZYMES (  136 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApaI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbeI        BbsI        BbvI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    Bsp1407I    
BspLU11I    BspMI       BsrBI       BsrDI       BsrGI       
BsrI        BssKI       BssSI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NarI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PleI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfoI        SmaI        
SmlI        SspBI       StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII       XmaCI       
XmaI