##################################
# NO CUT ENZYMES ( 38 enzymes) #
##################################
AccIII AclI AflII AscI AseI
AsnI BamHI BfrI BseAI BsiWI
BsmBI BspDI BspEI BssHII BstEII
BstPI ClaI CpoI EcoO65I EcoRV
FseI KspI MluI MroI NotI
NruI PacI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI SfiI
SnaBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 66 enzymes) #
###################################
AatII 3228 GACGTC GACGT^C
Acc65I 1440 GGTACC G^GTACC
AcsI 5908 GAATTC [GA]^AATT[CT]
AgeI 1167 ACCGGT A^CCGGT
AhdI 5193 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1212 GTGCAC G^TGCAC
Aor51HI 1361 AGCGCT AGC^GCT
ApaLI 1212 GTGCAC G^TGCAC
ApoI 5908 GAATTC [GA]^AATT[CT]
Asp700I 2606 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1440 GGTACC G^GTACC
AspEI 5193 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 477 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1912 TGCGCA TGC^GCA
AvrII 6574 CCTAGG C^CTAGG
BbrPI 1215 CACGTG CAC^GTG
BbvCI 3371 CCTCAGC CCTCAGC(-5/-2)
BcgI 4148 GCAGCGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3439 TGATCA T^GATCA
BlnI 6574 CCTAGG C^CTAGG
BsaAI 1215 CACGTG [CT]AC^GT[GA]
BsaBI 1448 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bst1107I 65 GTATAC GTA^TAC
BstBI 4156 TTCGAA TT^CGAA
BstZ17I 65 GTATAC GTA^TAC
DraI 5582 TTTAAA TTT^AAA
EagI 3156 CGGCCG C^GGCCG
Eam1105I 5193 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3156 CGGCCG C^GGCCG
Eco47III 1361 AGCGCT AGC^GCT
Eco52I 3156 CGGCCG C^GGCCG
EcoRI 5908 GAATTC G^AATTC
EcoT22I 1068 ATGCAT ATGCA^T
FbaI 3439 TGATCA T^GATCA
FspI 1912 TGCGCA TGC^GCA
HindIII 2407 AAGCTT A^AGCTT
HpaI 209 GTTAAC GTT^AAC
KpnI 1440 GGTACC GGTAC^C
MamI 1448 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3740 CAATTG C^AATTG
MunI 3740 CAATTG C^AATTG
NaeI 2243 GCCGGC GCC^GGC
NdeI 2491 CATATG CA^TATG
NgoMIV 2243 GCCGGC G^CCGGC
NheI 5512 GCTAGC G^CTAGC
NsiI 1068 ATGCAT ATGCA^T
NspV 4156 TTCGAA TT^CGAA
PaeR7I 2394 CTCGAG C^TCGAG
PflFI 477 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 416 CCAGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1167 ACCGGT A^CCGGT
PmaCI 1215 CACGTG CAC^GTG
PmlI 1215 CACGTG CAC^GTG
PshAI 3098 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 2702 CCTGCAGG CCTGCA^GG
ScaI 7218 AGTACT AGT^ACT
SexAI 3478 ACCTGGT A^CC[AT]GGT
SfuI 4156 TTCGAA TT^CGAA
SgrAI 1166 CACCGGTG C[GA]^CCGG[CT]G
SpeI 3311 ACTAGT A^CTAGT
Sse8387I 2702 CCTGCAGG CCTGCA^GG
Tth111I 477 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 416 CCAGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 6456 TCTAGA T^CTAGA
XhoI 2394 CTCGAG C^TCGAG
XmnI 2606 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 65 GTATAC GT^[AC][GT]AC
7110 GTAGAC
AocI 1664 CCTGAGG CC^T[ACGT]AGG
4868 CCTCAGG
BciVI 2104 GTATCC GTATCC(6/5)
6478 GTATCC
BglII 3544 AGATCT A^GATCT
4075 AGATCT
BsaWI 1167 ACCGGT [AT]^CCGG[AT]
1186 TCCGGT
BsmI 1472 GAATGC GAATGC(1/-1)
3053 GAATGC
BspHI 2692 TCATGA T^CATGA
5882 TCATGA
BsrFI 1167 ACCGGT [GA]^CCGG[CT]
2243 GCCGGC
Bsu36I 1664 CCTGAGG CC^T[ACGT]AGG
4868 CCTCAGG
Cfr10I 1167 ACCGGT [GA]^CCGG[CT]
2243 GCCGGC
DrdI 3222 GACGAGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3290 GACGGATGGGTC
Eco81I 1664 CCTGAGG CC^T[ACGT]AGG
4868 CCTCAGG
EcoNI 1319 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4540 CCTCCAGAAGG
HgaI 35 GACGC GACGC(5/10)
2946 GACGC
RcaI 2692 TCATGA T^CATGA
5882 TCATGA
SacI 4888 GAGCTC GAGCT^C
4972 GAGCTC
SapI 165 GAAGAGC GCTCTTC(1/4)
3734 GAAGAGC
SphI 1070 GCATGC GCATG^C
3388 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspLU11I BspMI BsrBI BsrDI BsrGI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI