################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AatII Acc65I AclI Aor51HI AscI Asp718I AviII AvrII BbrPI BcgI BlnI BsiWI BsmBI Bsp1407I BspDI BspLU11I BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI EagI EclXI Eco47III Eco52I EcoRV FseI FspI HpaI KpnI KspI MfeI MluI MunI NaeI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SphI Sse8387I SspBI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AcyI 483 GGCGCC G[GA]^CG[CT]C AflII 3114 CTTAAG C^TTAAG AflIII 2491 ACACGT A^C[GA][CT]GT AgeI 3825 ACCGGT A^CCGGT AhdI 662 GACCAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 549 ATTAAT AT^TAAT AsnI 549 ATTAAT AT^TAAT Asp700I 93 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 662 GACCAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3866 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 332 GGATCC G^GATCC BbeI 483 GGCGCC GGCGC^C BbsI 434 GAAGAC GAAGAC(2/6) BciVI 2748 GGATAC GTATCC(6/5) BfrI 3114 CTTAAG C^TTAAG BglII 2733 AGATCT A^GATCT BlpI 3656 GCTCAGC GC^T[ACGT]AGC Bpu1102I 3656 GCTCAGC GC^T[ACGT]AGC BpuAI 434 GAAGAC GAAGAC(2/6) BsaAI 693 TACGTA [CT]AC^GT[GA] BsaBI 170 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 483 GGCGCC G[GA]^CG[CT]C BsiEI 1839 CGACCG CG[GA][CT]^CG BsmI 204 GAATGC GAATGC(1/-1) BspHI 904 TCATGA T^CATGA BssSI 1915 CACGAG CACGAG(-5/-1) BstEII 2373 GGTGACC G^GT[ACGT]ACC BstPI 2373 GGTGACC G^GT[ACGT]ACC CelII 3656 GCTCAGC GC^T[ACGT]AGC DrdI 2292 GACACTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 662 GACCAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 2373 GGTGACC G^GT[ACGT]ACC EcoRI 2786 GAATTC G^AATTC EcoT22I 677 ATGCAT ATGCA^T HaeII 483 GGCGCC [GA]GCGC^[CT] Hin1I 483 GGCGCC G[GA]^CG[CT]C HincII 2043 GTCAAC GT[CT]^[GA]AC HindII 2043 GTCAAC GT[CT]^[GA]AC HindIII 96 AAGCTT A^AGCTT KasI 483 GGCGCC G^GCGCC MamI 170 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 483 GGCGCC GG^CGCC NheI 1937 GCTAGC G^CTAGC NsiI 677 ATGCAT ATGCA^T PflFI 3866 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 3825 ACCGGT A^CCGGT PshBI 549 ATTAAT AT^TAAT PstI 3318 CTGCAG CTGCA^G RcaI 904 TCATGA T^CATGA SfoI 483 GGCGCC GGC^GCC SmaI 952 CCCGGG CCC^GGG SnaBI 693 TACGTA TAC^GTA SpeI 1749 ACTAGT A^CTAGT Tth111I 3866 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1035 CCAGTGGACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 952 CCCGGG C^CCGGG XmaI 952 CCCGGG C^CCGGG XmnI 93 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccIII 1109 TCCGGA T^CCGGA 2608 TCCGGA Alw44I 816 GTGCAC G^TGCAC 2772 GTGCAC AlwNI 2764 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG 3157 CAGCTGCTG ApaLI 816 GTGCAC G^TGCAC 2772 GTGCAC BanI 483 GGCGCC G^G[CT][GA]CC 2876 GGCACC BclI 172 TGATCA T^GATCA 1132 TGATCA BsaI 660 GAGACC GGTCTC(1/5) 3019 GGTCTC BseAI 1109 TCCGGA T^CCGGA 2608 TCCGGA BspEI 1109 TCCGGA T^CCGGA 2608 TCCGGA BsrFI 1996 ACCGGC [GA]^CCGG[CT] 3825 ACCGGT BstAPI 643 GCATGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3783 GCACTAACTGC Cfr10I 1996 ACCGGC [GA]^CCGG[CT] 3825 ACCGGT FbaI 172 TGATCA T^GATCA 1132 TGATCA MroI 1109 TCCGGA T^CCGGA 2608 TCCGGA NspI 643 GCATGT [GA]CATG^[CT] 2327 GCATGT PflMI 1382 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2199 CCAGCCCTTGG SacI 1116 GAGCTC GAGCT^C 4281 GAGCTC Van91I 1382 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2199 CCAGCCCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI AocI ApaI ApoI AspHI AvaI AvaII BalI BanII BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII