##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AclI Aor51HI AscI
Asp718I AviII AvrII BbrPI BcgI
BlnI BsiWI BsmBI Bsp1407I BspDI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI EagI
EclXI Eco47III Eco52I EcoRV FseI
FspI HpaI KpnI KspI MfeI
MluI MunI NaeI NdeI NgoMIV
NotI NruI NspV PacI PaeR7I
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SphI Sse8387I SspBI SspI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AcyI 483 GGCGCC G[GA]^CG[CT]C
AflII 3114 CTTAAG C^TTAAG
AflIII 2491 ACACGT A^C[GA][CT]GT
AgeI 3825 ACCGGT A^CCGGT
AhdI 662 GACCAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 549 ATTAAT AT^TAAT
AsnI 549 ATTAAT AT^TAAT
Asp700I 93 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 662 GACCAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3866 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 332 GGATCC G^GATCC
BbeI 483 GGCGCC GGCGC^C
BbsI 434 GAAGAC GAAGAC(2/6)
BciVI 2748 GGATAC GTATCC(6/5)
BfrI 3114 CTTAAG C^TTAAG
BglII 2733 AGATCT A^GATCT
BlpI 3656 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3656 GCTCAGC GC^T[ACGT]AGC
BpuAI 434 GAAGAC GAAGAC(2/6)
BsaAI 693 TACGTA [CT]AC^GT[GA]
BsaBI 170 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 483 GGCGCC G[GA]^CG[CT]C
BsiEI 1839 CGACCG CG[GA][CT]^CG
BsmI 204 GAATGC GAATGC(1/-1)
BspHI 904 TCATGA T^CATGA
BssSI 1915 CACGAG CACGAG(-5/-1)
BstEII 2373 GGTGACC G^GT[ACGT]ACC
BstPI 2373 GGTGACC G^GT[ACGT]ACC
CelII 3656 GCTCAGC GC^T[ACGT]AGC
DrdI 2292 GACACTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 662 GACCAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2373 GGTGACC G^GT[ACGT]ACC
EcoRI 2786 GAATTC G^AATTC
EcoT22I 677 ATGCAT ATGCA^T
HaeII 483 GGCGCC [GA]GCGC^[CT]
Hin1I 483 GGCGCC G[GA]^CG[CT]C
HincII 2043 GTCAAC GT[CT]^[GA]AC
HindII 2043 GTCAAC GT[CT]^[GA]AC
HindIII 96 AAGCTT A^AGCTT
KasI 483 GGCGCC G^GCGCC
MamI 170 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 483 GGCGCC GG^CGCC
NheI 1937 GCTAGC G^CTAGC
NsiI 677 ATGCAT ATGCA^T
PflFI 3866 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3825 ACCGGT A^CCGGT
PshBI 549 ATTAAT AT^TAAT
PstI 3318 CTGCAG CTGCA^G
RcaI 904 TCATGA T^CATGA
SfoI 483 GGCGCC GGC^GCC
SmaI 952 CCCGGG CCC^GGG
SnaBI 693 TACGTA TAC^GTA
SpeI 1749 ACTAGT A^CTAGT
Tth111I 3866 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1035 CCAGTGGACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 952 CCCGGG C^CCGGG
XmaI 952 CCCGGG C^CCGGG
XmnI 93 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccIII 1109 TCCGGA T^CCGGA
2608 TCCGGA
Alw44I 816 GTGCAC G^TGCAC
2772 GTGCAC
AlwNI 2764 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
3157 CAGCTGCTG
ApaLI 816 GTGCAC G^TGCAC
2772 GTGCAC
BanI 483 GGCGCC G^G[CT][GA]CC
2876 GGCACC
BclI 172 TGATCA T^GATCA
1132 TGATCA
BsaI 660 GAGACC GGTCTC(1/5)
3019 GGTCTC
BseAI 1109 TCCGGA T^CCGGA
2608 TCCGGA
BspEI 1109 TCCGGA T^CCGGA
2608 TCCGGA
BsrFI 1996 ACCGGC [GA]^CCGG[CT]
3825 ACCGGT
BstAPI 643 GCATGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3783 GCACTAACTGC
Cfr10I 1996 ACCGGC [GA]^CCGG[CT]
3825 ACCGGT
FbaI 172 TGATCA T^GATCA
1132 TGATCA
MroI 1109 TCCGGA T^CCGGA
2608 TCCGGA
NspI 643 GCATGT [GA]CATG^[CT]
2327 GCATGT
PflMI 1382 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2199 CCAGCCCTTGG
SacI 1116 GAGCTC GAGCT^C
4281 GAGCTC
Van91I 1382 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2199 CCAGCCCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AocI ApaI ApoI
AspHI AvaI AvaII BalI BanII
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII