################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflII AgeI AscI AseI AsnI AspI BbeI BciVI BfrI BlpI Bpu1102I BsaBI BsaHI BseAI BsiEI BsiWI BspEI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII CpoI DrdI EagI EclXI Eco52I EcoO65I EcoRV EcoT22I FseI Hin1I KasI KspI MamI MluI MroI NarI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### Acc65I 605 GGTACC G^GTACC AlwNI 2797 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 3511 AGCGCT AGC^GCT ApaI 4089 GGGCCC GGGCC^C Asp718I 605 GGTACC G^GTACC AviII 2194 TGCGCA TGC^GCA BamHI 587 GGATCC G^GATCC BcgI 2543 GCAGAACTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 1165 GCCAGAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsmBI 742 GAGACG CGTCTC(1/5) BsmI 2647 GAATGC GAATGC(1/-1) BspDI 1677 ATCGAT AT^CGAT BspHI 3084 TCATGA T^CATGA BsrFI 53 GCCGGC [GA]^CCGG[CT] BssSI 5030 CTCGTG CACGAG(-5/-1) BstXI 415 CCAAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 53 GCCGGC [GA]^CCGG[CT] ClaI 1677 ATCGAT AT^CGAT Eco47III 3511 AGCGCT AGC^GCT FspI 2194 TGCGCA TGC^GCA HindIII 5069 AAGCTT A^AGCTT HpaI 950 GTTAAC GTT^AAC KpnI 605 GGTACC GGTAC^C MfeI 3150 CAATTG C^AATTG MunI 3150 CAATTG C^AATTG NaeI 53 GCCGGC GCC^GGC NcoI 4427 CCATGG C^CATGG NdeI 2420 CATATG CA^TATG NgoMIV 53 GCCGGC G^CCGGC RcaI 3084 TCATGA T^CATGA SexAI 3954 ACCTGGT A^CC[AT]GGT SmaI 2809 CCCGGG CCC^GGG XmaCI 2809 CCCGGG C^CCGGG XmaI 2809 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AhdI 203 GACTGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1091 GACCTTCTGTC Alw44I 4041 GTGCAC G^TGCAC 5581 GTGCAC AocI 3268 CCTCAGG CC^T[ACGT]AGG 3894 CCTCAGG ApaLI 4041 GTGCAC G^TGCAC 5581 GTGCAC AspEI 203 GACTGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1091 GACCTTCTGTC AvaI 2809 CCCGGG C^[CT]CG[GA]G 5401 CTCGGG AvrII 592 CCTAGG C^CTAGG 4598 CCTAGG BalI 477 TGGCCA TGG^CCA 3398 TGGCCA BglII 1768 AGATCT A^GATCT 2128 AGATCT BlnI 592 CCTAGG C^CTAGG 4598 CCTAGG BsaI 1824 GGTCTC GGTCTC(1/5) 3573 GGTCTC BsoBI 2809 CCCGGG C^[CT]CG[GA]G 5401 CTCGGG BspLU11I 1354 ACATGT A^CATGT 4399 ACATGT Bsu36I 3268 CCTCAGG CC^T[ACGT]AGG 3894 CCTCAGG DraIII 3302 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG 3634 CACTGAGTG DsaI 1201 CCACGG C^C[GA][CT]GG 4427 CCATGG Eam1105I 203 GACTGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1091 GACCTTCTGTC Eco81I 3268 CCTCAGG CC^T[ACGT]AGG 3894 CCTCAGG EcoNI 3955 CCTGGTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5235 CCTCCATGAGG EcoRI 2576 GAATTC G^AATTC 3028 GAATTC HaeII 3511 AGCGCT [GA]GCGC^[CT] 5037 GGCGCT MluNI 477 TGGCCA TGG^CCA 3398 TGGCCA MscI 477 TGGCCA TGG^CCA 3398 TGGCCA PflMI 3298 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4342 CCATCTTATGG SphI 578 GCATGC GCATG^C 2402 GCATGC SspI 2998 AATATT AAT^ATT 4556 AATATT StuI 2841 AGGCCT AGG^CCT 3166 AGGCCT Van91I 3298 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4342 CCATCTTATGG ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI ApoI Asp700I AspHI AvaII BanI BanII BbrPI BbsI BbvCI BbvI BclI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrBI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI