##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AgeI AscI AseI AsnI
AspI BbeI BciVI BfrI BlpI
Bpu1102I BsaBI BsaHI BseAI BsiEI
BsiWI BspEI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
CpoI DrdI EagI EclXI Eco52I
EcoO65I EcoRV EcoT22I FseI Hin1I
KasI KspI MamI MluI MroI
NarI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 605 GGTACC G^GTACC
AlwNI 2797 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 3511 AGCGCT AGC^GCT
ApaI 4089 GGGCCC GGGCC^C
Asp718I 605 GGTACC G^GTACC
AviII 2194 TGCGCA TGC^GCA
BamHI 587 GGATCC G^GATCC
BcgI 2543 GCAGAACTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1165 GCCAGAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmBI 742 GAGACG CGTCTC(1/5)
BsmI 2647 GAATGC GAATGC(1/-1)
BspDI 1677 ATCGAT AT^CGAT
BspHI 3084 TCATGA T^CATGA
BsrFI 53 GCCGGC [GA]^CCGG[CT]
BssSI 5030 CTCGTG CACGAG(-5/-1)
BstXI 415 CCAAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 53 GCCGGC [GA]^CCGG[CT]
ClaI 1677 ATCGAT AT^CGAT
Eco47III 3511 AGCGCT AGC^GCT
FspI 2194 TGCGCA TGC^GCA
HindIII 5069 AAGCTT A^AGCTT
HpaI 950 GTTAAC GTT^AAC
KpnI 605 GGTACC GGTAC^C
MfeI 3150 CAATTG C^AATTG
MunI 3150 CAATTG C^AATTG
NaeI 53 GCCGGC GCC^GGC
NcoI 4427 CCATGG C^CATGG
NdeI 2420 CATATG CA^TATG
NgoMIV 53 GCCGGC G^CCGGC
RcaI 3084 TCATGA T^CATGA
SexAI 3954 ACCTGGT A^CC[AT]GGT
SmaI 2809 CCCGGG CCC^GGG
XmaCI 2809 CCCGGG C^CCGGG
XmaI 2809 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AhdI 203 GACTGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1091 GACCTTCTGTC
Alw44I 4041 GTGCAC G^TGCAC
5581 GTGCAC
AocI 3268 CCTCAGG CC^T[ACGT]AGG
3894 CCTCAGG
ApaLI 4041 GTGCAC G^TGCAC
5581 GTGCAC
AspEI 203 GACTGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1091 GACCTTCTGTC
AvaI 2809 CCCGGG C^[CT]CG[GA]G
5401 CTCGGG
AvrII 592 CCTAGG C^CTAGG
4598 CCTAGG
BalI 477 TGGCCA TGG^CCA
3398 TGGCCA
BglII 1768 AGATCT A^GATCT
2128 AGATCT
BlnI 592 CCTAGG C^CTAGG
4598 CCTAGG
BsaI 1824 GGTCTC GGTCTC(1/5)
3573 GGTCTC
BsoBI 2809 CCCGGG C^[CT]CG[GA]G
5401 CTCGGG
BspLU11I 1354 ACATGT A^CATGT
4399 ACATGT
Bsu36I 3268 CCTCAGG CC^T[ACGT]AGG
3894 CCTCAGG
DraIII 3302 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
3634 CACTGAGTG
DsaI 1201 CCACGG C^C[GA][CT]GG
4427 CCATGG
Eam1105I 203 GACTGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1091 GACCTTCTGTC
Eco81I 3268 CCTCAGG CC^T[ACGT]AGG
3894 CCTCAGG
EcoNI 3955 CCTGGTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5235 CCTCCATGAGG
EcoRI 2576 GAATTC G^AATTC
3028 GAATTC
HaeII 3511 AGCGCT [GA]GCGC^[CT]
5037 GGCGCT
MluNI 477 TGGCCA TGG^CCA
3398 TGGCCA
MscI 477 TGGCCA TGG^CCA
3398 TGGCCA
PflMI 3298 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4342 CCATCTTATGG
SphI 578 GCATGC GCATG^C
2402 GCATGC
SspI 2998 AATATT AAT^ATT
4556 AATATT
StuI 2841 AGGCCT AGG^CCT
3166 AGGCCT
Van91I 3298 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4342 CCATCTTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI Asp700I AspHI
AvaII BanI BanII BbrPI BbsI
BbvCI BbvI BclI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspMI BsrBI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI