##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AccIII AclI AgeI AhdI AscI
AspEI AspI AviII AvrII BamHI
BbeI BbvCI BcgI BciVI BclI
BlnI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoO65I FbaI FseI
FspI HpaI KasI KspI MluI
MroI NarI NheI NotI NspV
PacI PflFI PinAI PpuMI PshAI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI Tth111I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 816 GACGTC GACGT^C
Acc65I 3347 GGTACC G^GTACC
AccII 1347 CGCG CG^CG
AflII 2763 CTTAAG C^TTAAG
Alw44I 3298 GTGCAC G^TGCAC
AocI 1062 CCTGAGG CC^T[ACGT]AGG
Aor51HI 751 AGCGCT AGC^GCT
ApaLI 3298 GTGCAC G^TGCAC
AseI 5539 ATTAAT AT^TAAT
AsnI 5539 ATTAAT AT^TAAT
Asp718I 3347 GGTACC G^GTACC
BalI 760 TGGCCA TGG^CCA
BbrPI 447 CACGTG CAC^GTG
BfrI 2763 CTTAAG C^TTAAG
BglI 898 GCCTGACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2021 AGATCT A^GATCT
BlpI 120 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 120 GCTGAGC GC^T[ACGT]AGC
BsaAI 447 CACGTG [CT]AC^GT[GA]
BsaBI 1850 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2374 CGTCTC CGTCTC(1/5)
Bsp1407I 5824 TGTACA T^GTACA
BsrFI 2107 GCCGGC [GA]^CCGG[CT]
BsrGI 5824 TGTACA T^GTACA
BstUI 1347 CGCG CG^CG
Bsu36I 1062 CCTGAGG CC^T[ACGT]AGG
CelII 120 GCTGAGC GC^T[ACGT]AGC
CfoI 752 GCGC GCG^C
Cfr10I 2107 GCCGGC [GA]^CCGG[CT]
EaeI 760 TGGCCA [CT]^GGCC[GA]
Eco47III 751 AGCGCT AGC^GCT
Eco81I 1062 CCTGAGG CC^T[ACGT]AGG
EcoNI 4230 CCTGTTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 309 GATATC GAT^ATC
HaeII 751 AGCGCT [GA]GCGC^[CT]
HgaI 3648 GACGC GACGC(5/10)
HhaI 752 GCGC GCG^C
HinP1I 752 GCGC G^CGC
KpnI 3347 GGTACC GGTAC^C
MamI 1850 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 760 TGGCCA TGG^CCA
MscI 760 TGGCCA TGG^CCA
MspA1I 2034 CAGCTG C[AC]G^C[GT]G
MvnI 1347 CGCG CG^CG
NaeI 2107 GCCGGC GCC^GGC
NgoMIV 2107 GCCGGC G^CCGGC
NruI 1346 TCGCGA TCG^CGA
PaeR7I 360 CTCGAG C^TCGAG
PmaCI 447 CACGTG CAC^GTG
PmeI 3064 GTTTAAAC GTTT^AAAC
PmlI 447 CACGTG CAC^GTG
PshBI 5539 ATTAAT AT^TAAT
PvuII 2034 CAGCTG CAG^CTG
SapI 210 GCTCTTC GCTCTTC(1/4)
SfiI 897 GGCCTGACGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 5824 TGTACA T^GTACA
XcmI 4519 CCATTAGATTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 360 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 4881 GTAGAC GT^[AC][GT]AC
5271 GTAGAC
AcyI 816 GACGTC G[GA]^CG[CT]C
3648 GACGCC
AflIII 2813 ACATGT A^C[GA][CT]GT
5116 ACATGT
ApaI 2243 GGGCCC GGGCC^C
4452 GGGCCC
BcnI 527 CCGGG CC^[GC]GG
4636 CCGGG
BsaHI 816 GACGTC G[GA]^CG[CT]C
3648 GACGCC
BseRI 2126 CTCCTC GAGGAG(10/8)
549 GAGGAG
BsgI 936 CTGCAC GTGCAG(16/14)
3628 CTGCAC
BspLU11I 2813 ACATGT A^CATGT
5116 ACATGT
BsrBI 208 CCGCTC CCGCTC(-3/-3)
3899 CCGCTC
EcoRI 6 GAATTC G^AATTC
2399 GAATTC
EcoT14I 11 CCATGG C^C[AT][AT]GG
2349 CCATGG
Hin1I 816 GACGTC G[GA]^CG[CT]C
3648 GACGCC
HincII 1224 GTCAAC GT[CT]^[GA]AC
4861 GTCAAC
HindII 1224 GTCAAC GT[CT]^[GA]AC
4861 GTCAAC
HindIII 2996 AAGCTT A^AGCTT
4017 AAGCTT
MfeI 845 CAATTG C^AATTG
3229 CAATTG
MunI 845 CAATTG C^AATTG
3229 CAATTG
NciI 527 CCGGG CC^[GC]GG
4636 CCGGG
NcoI 11 CCATGG C^CATGG
2349 CCATGG
NdeI 790 CATATG CA^TATG
3563 CATATG
PflMI 2033 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2578 CCACTTACTGG
StuI 2470 AGGCCT AGG^CCT
3460 AGGCCT
StyI 11 CCATGG C^C[AT][AT]GG
2349 CCATGG
Van91I 2033 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2578 CCACTTACTGG
XbaI 3022 TCTAGA T^CTAGA
4146 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaI
AvaII BanI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoO109I EcoRII EcoT22I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
NsiI NspI PleI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI