##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI ApaI AscI Asp718I AspI
AviII BanI BbeI BbrPI BclI
BglI BsaAI BsaBI BsaHI BseAI
Bsp1407I BspDI BspEI BsrBI BsrDI
BsrFI BsrGI BssHII BstAPI BstEII
BstPI Cfr10I ClaI EagI EclXI
Eco52I EcoO65I EcoRI FbaI FseI
FspI Hin1I KasI KpnI KspI
MamI MluI MroI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI SacII SalI
ScaI SfiI SfoI SgrAI SmaI
SnaBI SpeI SspBI StuI Tth111I
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AocI 1118 CCTCAGG CC^T[ACGT]AGG
Aor51HI 826 AGCGCT AGC^GCT
BamHI 4460 GGATCC G^GATCC
BbsI 3838 GTCTTC GAAGAC(2/6)
BcgI 4021 GCATCACTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1772 AGATCT A^GATCT
BlpI 3014 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3014 GCTAAGC GC^T[ACGT]AGC
BpuAI 3838 GTCTTC GAAGAC(2/6)
BsaWI 2425 TCCGGT [AT]^CCGG[AT]
BsgI 2147 GTGCAG GTGCAG(16/14)
BsiEI 35 CGGTCG CG[GA][CT]^CG
BsiWI 25 CGTACG C^GTACG
BsmBI 139 GAGACG CGTCTC(1/5)
BsmI 30 GCATTC GAATGC(1/-1)
Bst1107I 1548 GTATAC GTA^TAC
BstBI 1379 TTCGAA TT^CGAA
BstXI 2037 CCAGGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1548 GTATAC GTA^TAC
Bsu36I 1118 CCTCAGG CC^T[ACGT]AGG
CelII 3014 GCTAAGC GC^T[ACGT]AGC
CpoI 178 CGGTCCG CG^G[AT]CCG
DsaI 171 CCACGG C^C[GA][CT]GG
Eco47III 826 AGCGCT AGC^GCT
Eco81I 1118 CCTCAGG CC^T[ACGT]AGG
HincII 2812 GTTAAC GT[CT]^[GA]AC
HindII 2812 GTTAAC GT[CT]^[GA]AC
HpaI 2812 GTTAAC GTT^AAC
MfeI 885 CAATTG C^AATTG
MunI 885 CAATTG C^AATTG
NspV 1379 TTCGAA TT^CGAA
PacI 1298 TTAATTAA TTAAT^TAA
PaeR7I 1707 CTCGAG C^TCGAG
RsrII 178 CGGTCCG CG^G[AT]CCG
SacI 566 GAGCTC GAGCT^C
SbfI 3351 CCTGCAGG CCTGCA^GG
SfuI 1379 TTCGAA TT^CGAA
Sse8387I 3351 CCTGCAGG CCTGCA^GG
SspI 1860 AATATT AAT^ATT
SwaI 1875 ATTTAAAT ATTT^AAAT
TfiI 3987 GAATC G^A[AT]TC
XbaI 4427 TCTAGA T^CTAGA
XhoI 1707 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 44 enzymes) #
###################################
AflII 404 CTTAAG C^TTAAG
1497 CTTAAG
AflIII 3463 ACATGT A^C[GA][CT]GT
3625 ACATGT
AhdI 770 GACGTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2503 GACTCCCAGTC
Alw44I 942 GTGCAC G^TGCAC
1222 GTGCAC
ApaLI 942 GTGCAC G^TGCAC
1222 GTGCAC
AseI 1297 ATTAAT AT^TAAT
2229 ATTAAT
AsnI 1297 ATTAAT AT^TAAT
2229 ATTAAT
Asp700I 824 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2913 GAAATGCTTC
AspEI 770 GACGTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2503 GACTCCCAGTC
AvrII 242 CCTAGG C^CTAGG
3434 CCTAGG
BalI 297 TGGCCA TGG^CCA
3481 TGGCCA
BbvCI 89 GCTGAGG CCTCAGC(-5/-2)
1953 GCTGAGG
BciVI 2352 GGATAC GTATCC(6/5)
4361 GGATAC
BfrI 404 CTTAAG C^TTAAG
1497 CTTAAG
BlnI 242 CCTAGG C^CTAGG
3434 CCTAGG
BpmI 2434 CTCCAG CTGGAG(16/14)
3021 CTGGAG
BspHI 1136 TCATGA T^CATGA
2934 TCATGA
BspLU11I 3463 ACATGT A^CATGT
3625 ACATGT
BssSI 569 CTCGTG CACGAG(-5/-1)
3344 CTCGTG
BstYI 1772 AGATCT [GA]^GATC[CT]
4460 GGATCC
DrdI 920 GACACCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3370 GACCACATGGTC
Eam1105I 770 GACGTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2503 GACTCCCAGTC
EcoNI 325 CCTCTGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3429 CCTTTCCTAGG
EcoRV 2017 GATATC GAT^ATC
3040 GATATC
EcoT22I 2389 ATGCAT ATGCA^T
4019 ATGCAT
FauI 3169 CCCGC CCCGC(4/6)
3221 GCGGG
HaeII 75 AGCGCC [GA]GCGC^[CT]
826 AGCGCT
HgaI 141 GACGC GACGC(5/10)
3334 GACGC
HindIII 1103 AAGCTT A^AGCTT
3812 AAGCTT
MflI 1772 AGATCT [GA]^GATC[CT]
4460 GGATCC
MluNI 297 TGGCCA TGG^CCA
3481 TGGCCA
MscI 297 TGGCCA TGG^CCA
3481 TGGCCA
NheI 3961 GCTAGC G^CTAGC
4173 GCTAGC
NsiI 2389 ATGCAT ATGCA^T
4019 ATGCAT
PflMI 851 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1342 CCAGCACTTGG
PshBI 1297 ATTAAT AT^TAAT
2229 ATTAAT
PvuII 311 CAGCTG CAG^CTG
641 CAGCTG
RcaI 1136 TCATGA T^CATGA
2934 TCATGA
SapI 4062 GAAGAGC GCTCTTC(1/4)
877 GCTCTTC
SexAI 1087 ACCTGGT A^CC[AT]GGT
1530 ACCTGGT
SphI 706 GCATGC GCATG^C
1539 GCATGC
Van91I 851 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1342 CCAGCACTTGG
XhoII 1772 AGATCT [GA]^GATC[CT]
4460 GGATCC
XmnI 824 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2913 GAAATGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanII BbvI BcnI
BfaI BmyI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I