################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## Acc65I AccIII AflII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AspI AvrII BalI BfrI BglII BlnI BsaBI BseAI BsiEI BspDI BspEI BspLU11I BstBI ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FseI HpaI KpnI MamI MfeI MluNI MroI MscI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI PvuI RsrII SalI SbfI SfiI SfuI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AatII 1718 GACGTC GACGT^C AccI 2289 GTATAC GT^[AC][GT]AC AclI 2362 AACGTT AA^CGTT AcsI 3156 GAATTC [GA]^AATT[CT] AocI 475 CCTCAGG CC^T[ACGT]AGG Aor51HI 201 AGCGCT AGC^GCT ApaI 2075 GGGCCC GGGCC^C ApoI 3156 GAATTC [GA]^AATT[CT] Asp700I 2274 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 1147 TGCGCA TGC^GCA BbeI 2476 GGCGCC GGCGC^C BbrPI 1885 CACGTG CAC^GTG BbvCI 749 GCTGAGG CCTCAGC(-5/-2) BcgI 1024 GCAGCCAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1771 GGATAC GTATCC(6/5) BclI 1710 TGATCA T^GATCA BsaAI 1885 CACGTG [CT]AC^GT[GA] BsiWI 2185 CGTACG C^GTACG BsmI 1602 GAATGC GAATGC(1/-1) BspHI 3160 TCATGA T^CATGA BssHII 72 GCGCGC G^CGCGC Bst1107I 2289 GTATAC GTA^TAC BstEII 2208 GGTCACC G^GT[ACGT]ACC BstPI 2208 GGTCACC G^GT[ACGT]ACC BstXI 1893 CCAGCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 2289 GTATAC GTA^TAC Bsu36I 475 CCTCAGG CC^T[ACGT]AGG DrdI 1802 GACGCCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 201 AGCGCT AGC^GCT Eco81I 475 CCTCAGG CC^T[ACGT]AGG EcoNI 1411 CCTCTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2208 GGTCACC G^GT[ACGT]ACC EcoRI 3156 GAATTC G^AATTC FbaI 1710 TGATCA T^GATCA FspI 1147 TGCGCA TGC^GCA HindIII 1520 AAGCTT A^AGCTT KasI 2476 GGCGCC G^GCGCC KspI 64 CCGCGG CCGC^GG MluI 88 ACGCGT A^CGCGT MslI 2136 CATCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 2476 GGCGCC GG^CGCC PflMI 622 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1885 CACGTG CAC^GTG PmlI 1885 CACGTG CAC^GTG Psp1406I 2362 AACGTT AA^CGTT RcaI 3160 TCATGA T^CATGA SacII 64 CCGCGG CCGC^GG ScaI 3170 AGTACT AGT^ACT SexAI 2711 ACCTGGT A^CC[AT]GGT SfoI 2476 GGCGCC GGC^GCC SgrAI 105 CGCCGGCG C[GA]^CCGG[CT]G StuI 714 AGGCCT AGG^CCT Van91I 622 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1158 CCAAGTACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 2274 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AflIII 88 ACGCGT A^C[GA][CT]GT 928 ACGTGT BamHI 2390 GGATCC G^GATCC 2538 GGATCC BlpI 157 GCTGAGC GC^T[ACGT]AGC 344 GCTGAGC Bpu1102I 157 GCTGAGC GC^T[ACGT]AGC 344 GCTGAGC BsaWI 678 ACCGGA [AT]^CCGG[AT] 1548 TCCGGT BsmBI 617 CGTCTC CGTCTC(1/5) 463 GAGACG Bsp1407I 2729 TGTACA T^GTACA 2784 TGTACA BspMI 2027 ACCTGC ACCTGC(4/8) 2254 GCAGGT BsrDI 1339 CATTGC GCAATG(2/0) 2103 CATTGC BsrGI 2729 TGTACA T^GTACA 2784 TGTACA BssSI 1673 CACGAG CACGAG(-5/-1) 3280 CACGAG BstAPI 142 GCACTCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 229 GCAGCGCCTGC CelII 157 GCTGAGC GC^T[ACGT]AGC 344 GCTGAGC DraIII 143 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG 2733 CACACAGTG HincII 2623 GTCAAC GT[CT]^[GA]AC 2683 GTCAAC HindII 2623 GTCAAC GT[CT]^[GA]AC 2683 GTCAAC MseI 2912 TTAA T^TAA 3346 TTAA NaeI 106 GCCGGC GCC^GGC 268 GCCGGC NcoI 96 CCATGG C^CATGG 768 CCATGG NgoMIV 106 GCCGGC G^CCGGC 268 GCCGGC NspI 1002 ACATGC [GA]CATG^[CT] 2039 ACATGC PstI 1876 CTGCAG CTGCA^G 2217 CTGCAG SapI 2169 GAAGAGC GCTCTTC(1/4) 3285 GAAGAGC SspBI 2729 TGTACA T^GTACA 2784 TGTACA Tru9I 2912 TTAA T^TAA 3346 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI