##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AccIII AflII AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvrII BalI
BfrI BglII BlnI BsaBI BseAI
BsiEI BspDI BspEI BspLU11I BstBI
ClaI CpoI DraI EagI Eam1105I
EclXI Eco52I EcoRV EcoT22I FseI
HpaI KpnI MamI MfeI MluNI
MroI MscI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI PvuI RsrII SalI SbfI
SfiI SfuI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 1718 GACGTC GACGT^C
AccI 2289 GTATAC GT^[AC][GT]AC
AclI 2362 AACGTT AA^CGTT
AcsI 3156 GAATTC [GA]^AATT[CT]
AocI 475 CCTCAGG CC^T[ACGT]AGG
Aor51HI 201 AGCGCT AGC^GCT
ApaI 2075 GGGCCC GGGCC^C
ApoI 3156 GAATTC [GA]^AATT[CT]
Asp700I 2274 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1147 TGCGCA TGC^GCA
BbeI 2476 GGCGCC GGCGC^C
BbrPI 1885 CACGTG CAC^GTG
BbvCI 749 GCTGAGG CCTCAGC(-5/-2)
BcgI 1024 GCAGCCAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1771 GGATAC GTATCC(6/5)
BclI 1710 TGATCA T^GATCA
BsaAI 1885 CACGTG [CT]AC^GT[GA]
BsiWI 2185 CGTACG C^GTACG
BsmI 1602 GAATGC GAATGC(1/-1)
BspHI 3160 TCATGA T^CATGA
BssHII 72 GCGCGC G^CGCGC
Bst1107I 2289 GTATAC GTA^TAC
BstEII 2208 GGTCACC G^GT[ACGT]ACC
BstPI 2208 GGTCACC G^GT[ACGT]ACC
BstXI 1893 CCAGCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2289 GTATAC GTA^TAC
Bsu36I 475 CCTCAGG CC^T[ACGT]AGG
DrdI 1802 GACGCCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 201 AGCGCT AGC^GCT
Eco81I 475 CCTCAGG CC^T[ACGT]AGG
EcoNI 1411 CCTCTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2208 GGTCACC G^GT[ACGT]ACC
EcoRI 3156 GAATTC G^AATTC
FbaI 1710 TGATCA T^GATCA
FspI 1147 TGCGCA TGC^GCA
HindIII 1520 AAGCTT A^AGCTT
KasI 2476 GGCGCC G^GCGCC
KspI 64 CCGCGG CCGC^GG
MluI 88 ACGCGT A^CGCGT
MslI 2136 CATCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 2476 GGCGCC GG^CGCC
PflMI 622 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1885 CACGTG CAC^GTG
PmlI 1885 CACGTG CAC^GTG
Psp1406I 2362 AACGTT AA^CGTT
RcaI 3160 TCATGA T^CATGA
SacII 64 CCGCGG CCGC^GG
ScaI 3170 AGTACT AGT^ACT
SexAI 2711 ACCTGGT A^CC[AT]GGT
SfoI 2476 GGCGCC GGC^GCC
SgrAI 105 CGCCGGCG C[GA]^CCGG[CT]G
StuI 714 AGGCCT AGG^CCT
Van91I 622 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1158 CCAAGTACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2274 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 88 ACGCGT A^C[GA][CT]GT
928 ACGTGT
BamHI 2390 GGATCC G^GATCC
2538 GGATCC
BlpI 157 GCTGAGC GC^T[ACGT]AGC
344 GCTGAGC
Bpu1102I 157 GCTGAGC GC^T[ACGT]AGC
344 GCTGAGC
BsaWI 678 ACCGGA [AT]^CCGG[AT]
1548 TCCGGT
BsmBI 617 CGTCTC CGTCTC(1/5)
463 GAGACG
Bsp1407I 2729 TGTACA T^GTACA
2784 TGTACA
BspMI 2027 ACCTGC ACCTGC(4/8)
2254 GCAGGT
BsrDI 1339 CATTGC GCAATG(2/0)
2103 CATTGC
BsrGI 2729 TGTACA T^GTACA
2784 TGTACA
BssSI 1673 CACGAG CACGAG(-5/-1)
3280 CACGAG
BstAPI 142 GCACTCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
229 GCAGCGCCTGC
CelII 157 GCTGAGC GC^T[ACGT]AGC
344 GCTGAGC
DraIII 143 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
2733 CACACAGTG
HincII 2623 GTCAAC GT[CT]^[GA]AC
2683 GTCAAC
HindII 2623 GTCAAC GT[CT]^[GA]AC
2683 GTCAAC
MseI 2912 TTAA T^TAA
3346 TTAA
NaeI 106 GCCGGC GCC^GGC
268 GCCGGC
NcoI 96 CCATGG C^CATGG
768 CCATGG
NgoMIV 106 GCCGGC G^CCGGC
268 GCCGGC
NspI 1002 ACATGC [GA]CATG^[CT]
2039 ACATGC
PstI 1876 CTGCAG CTGCA^G
2217 CTGCAG
SapI 2169 GAAGAGC GCTCTTC(1/4)
3285 GAAGAGC
SspBI 2729 TGTACA T^GTACA
2784 TGTACA
Tru9I 2912 TTAA T^TAA
3346 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmaCI XmaI