##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AccII AccIII AcyI AgeI
Aor51HI ApaI AscI AspI AvaI
AviII BbeI BbvCI BcgI BcnI
BglI BsaHI BseAI BsiWI BsoBI
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BssHII BstAPI BstBI BstUI
Cfr10I ClaI CpoI EagI EclXI
Eco47III Eco52I EcoT22I FseI FspI
HaeII Hin1I KasI KspI MfeI
MluI MroI MunI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PvuI
RcaI RsrII SacII SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
Sse8387I StuI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 4014 GGTACC G^GTACC
AhdI 5527 GACAGGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2065 GTGCAC G^TGCAC
AlwNI 929 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 643 CCTCAGG CC^T[ACGT]AGG
ApaLI 2065 GTGCAC G^TGCAC
Asp718I 4014 GGTACC G^GTACC
AspEI 5527 GACAGGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 5288 TGGCCA TGG^CCA
BamHI 985 GGATCC G^GATCC
BanII 2504 GAGCTC G[GA]GC[CT]^C
BlpI 4338 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 4338 GCTTAGC GC^T[ACGT]AGC
BsaI 298 GGTCTC GGTCTC(1/5)
BsiEI 155 CGACCG CG[GA][CT]^CG
BsmBI 5419 CGTCTC CGTCTC(1/5)
Bsp1407I 6257 TGTACA T^GTACA
BsrGI 6257 TGTACA T^GTACA
BssSI 2250 CTCGTG CACGAG(-5/-1)
BstEII 3129 GGTAACC G^GT[ACGT]ACC
BstPI 3129 GGTAACC G^GT[ACGT]ACC
Bsu36I 643 CCTCAGG CC^T[ACGT]AGG
CelII 4338 GCTTAGC GC^T[ACGT]AGC
CfoI 707 GCGC GCG^C
DraII 639 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 4932 GACATTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1772 CCATGG C^C[GA][CT]GG
EaeI 5288 TGGCCA [CT]^GGCC[GA]
Eam1105I 5527 GACAGGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 643 CCTCAGG CC^T[ACGT]AGG
EcoO109I 639 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 3129 GGTAACC G^GT[ACGT]ACC
EcoRV 3220 GATATC GAT^ATC
FauI 606 CCCGC CCCGC(4/6)
HgaI 5418 GCGTC GACGC(5/10)
HhaI 707 GCGC GCG^C
HinP1I 707 GCGC G^CGC
KpnI 4014 GGTACC GGTAC^C
MluNI 5288 TGGCCA TGG^CCA
MscI 5288 TGGCCA TGG^CCA
NcoI 1772 CCATGG C^CATGG
NdeI 485 CATATG CA^TATG
PpuMI 639 AGGACCT [GA]G^G[AT]CC[CT]
PstI 404 CTGCAG CTGCA^G
PvuII 1735 CAGCTG CAG^CTG
SacI 2504 GAGCTC GAGCT^C
SalI 5788 GTCGAC G^TCGAC
ScaI 4670 AGTACT AGT^ACT
SnaBI 2806 TACGTA TAC^GTA
SphI 4659 GCATGC GCATG^C
SspBI 6257 TGTACA T^GTACA
SwaI 2080 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AclI 250 AACGTT AA^CGTT
5208 AACGTT
AflIII 1399 ACACGT A^C[GA][CT]GT
4025 ACACGT
AvrII 4430 CCTAGG C^CTAGG
4645 CCTAGG
BbrPI 1400 CACGTG CAC^GTG
4026 CACGTG
BbsI 2182 GAAGAC GAAGAC(2/6)
3937 GAAGAC
BciVI 3999 GTATCC GTATCC(6/5)
6169 GTATCC
BclI 1792 TGATCA T^GATCA
6310 TGATCA
BlnI 4430 CCTAGG C^CTAGG
4645 CCTAGG
BpuAI 2182 GAAGAC GAAGAC(2/6)
3937 GAAGAC
BsaWI 157 ACCGGA [AT]^CCGG[AT]
697 ACCGGA
BsmI 6013 GAATGC GAATGC(1/-1)
2900 GCATTC
BspMI 3844 ACCTGC ACCTGC(4/8)
2488 GCAGGT
Bst1107I 2652 GTATAC GTA^TAC
5264 GTATAC
BstXI 2339 CCAAGCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5872 CCATACAGGTGG
BstZ17I 2652 GTATAC GTA^TAC
5264 GTATAC
DraIII 2963 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
5683 CACTGTGTG
EcoNI 1662 CCTGATTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4627 CCTTTTGTAGG
EcoRI 4806 GAATTC G^AATTC
5256 GAATTC
FbaI 1792 TGATCA T^GATCA
6310 TGATCA
HindIII 816 AAGCTT A^AGCTT
3988 AAGCTT
HpaI 209 GTTAAC GTT^AAC
4853 GTTAAC
MspA1I 1735 CAGCTG C[AC]G^C[GT]G
2635 CAGCGG
NspI 4343 GCATGT [GA]CATG^[CT]
4659 GCATGC
PmaCI 1400 CACGTG CAC^GTG
4026 CACGTG
PmlI 1400 CACGTG CAC^GTG
4026 CACGTG
Psp1406I 250 AACGTT AA^CGTT
5208 AACGTT
SapI 2501 GAAGAGC GCTCTTC(1/4)
3848 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI ApoI AseI AsnI
Asp700I AspHI AvaII BanI BbvI
BfaI BfrI BglII BmyI BpmI
BsaAI BsaBI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV PflMI PleI PshBI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SpeI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XcmI XhoII XmnI