################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AhdI AocI AscI Asp718I AspEI BbeI BbvCI BcgI BglI BsaHI BsiEI BsiWI BsmI BsrBI Bst1107I BstBI BstZ17I Bsu36I CpoI EagI Eam1105I EclXI Eco52I Eco81I EcoT22I FseI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PmeI PshAI Psp1406I PvuI RsrII SacI SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SwaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 927 CGCG CG^CG AccIII 1888 TCCGGA T^CCGGA AflII 3971 CTTAAG C^TTAAG AgeI 459 ACCGGT A^CCGGT Alw44I 4197 GTGCAC G^TGCAC ApaLI 4197 GTGCAC G^TGCAC AspHI 4197 GTGCAC G[AT]GC[AT]^C AviII 4493 TGCGCA TGC^GCA AvrII 2211 CCTAGG C^CTAGG BciVI 2374 GGATAC GTATCC(6/5) BfrI 3971 CTTAAG C^TTAAG BglII 4420 AGATCT A^GATCT BlnI 2211 CCTAGG C^CTAGG BlpI 657 GCTCAGC GC^T[ACGT]AGC Bpu1102I 657 GCTCAGC GC^T[ACGT]AGC BsaBI 2028 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 1888 TCCGGA T^CCGGA BsiHKAI 4197 GTGCAC G[AT]GC[AT]^C BsmBI 1372 GAGACG CGTCTC(1/5) BspDI 2492 ATCGAT AT^CGAT BspEI 1888 TCCGGA T^CCGGA BspLU11I 4465 ACATGT A^CATGT BssHII 926 GCGCGC G^CGCGC BstAPI 904 GCAAGCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 2856 GGTGACC G^GT[ACGT]ACC BstPI 2856 GGTGACC G^GT[ACGT]ACC BstUI 927 CGCG CG^CG CelII 657 GCTCAGC GC^T[ACGT]AGC ClaI 2492 ATCGAT AT^CGAT DrdI 1681 GACCCCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 2856 GGTGACC G^GT[ACGT]ACC EcoRV 1699 GATATC GAT^ATC FauI 1061 CCCGC CCCGC(4/6) FspI 4493 TGCGCA TGC^GCA HgaI 1374 GACGC GACGC(5/10) MamI 2028 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1888 TCCGGA T^CCGGA MvnI 927 CGCG CG^CG NcoI 1793 CCATGG C^CATGG NspI 4465 ACATGT [GA]CATG^[CT] PaeR7I 1986 CTCGAG C^TCGAG PinAI 459 ACCGGT A^CCGGT SapI 1960 GAAGAGC GCTCTTC(1/4) SpeI 801 ACTAGT A^CTAGT StuI 3756 AGGCCT AGG^CCT XbaI 3198 TCTAGA T^CTAGA XhoI 1986 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AflIII 933 ACACGT A^C[GA][CT]GT 4465 ACATGT Aor51HI 1919 AGCGCT AGC^GCT 3163 AGCGCT AseI 1468 ATTAAT AT^TAAT 3383 ATTAAT AsnI 1468 ATTAAT AT^TAAT 3383 ATTAAT BamHI 475 GGATCC G^GATCC 2603 GGATCC BclI 3694 TGATCA T^GATCA 3915 TGATCA BsgI 511 CTGCAC GTGCAG(16/14) 2531 GTGCAG Bsp1407I 468 TGTACA T^GTACA 4715 TGTACA BspHI 2658 TCATGA T^CATGA 4651 TCATGA BsrDI 909 CATTGC GCAATG(2/0) 4278 CATTGC BsrGI 468 TGTACA T^GTACA 4715 TGTACA BstXI 200 CCACAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1256 CCAGCACTGTGG DraIII 1627 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG 2851 CACAAGGTG Eco47III 1919 AGCGCT AGC^GCT 3163 AGCGCT EcoNI 294 CCTGCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2984 CCTACGACAGG FbaI 3694 TGATCA T^GATCA 3915 TGATCA HaeII 1919 AGCGCT [GA]GCGC^[CT] 3163 AGCGCT MslI 201 CACAGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 966 CACGAGTATG NaeI 3129 GCCGGC GCC^GGC 4218 GCCGGC NgoMIV 3129 GCCGGC G^CCGGC 4218 GCCGGC PshBI 1468 ATTAAT AT^TAAT 3383 ATTAAT PstI 1348 CTGCAG CTGCA^G 4175 CTGCAG RcaI 2658 TCATGA T^CATGA 4651 TCATGA SspBI 468 TGTACA T^GTACA 4715 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApaI ApoI Asp700I AspI AvaI AvaII BalI BanI BanII BbrPI BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflFI PflMI PleI PmaCI PmlI PpuMI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SexAI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XhoII XmnI