##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AhdI AocI AscI Asp718I AspEI
BbeI BbvCI BcgI BglI BsaHI
BsiEI BsiWI BsmI BsrBI Bst1107I
BstBI BstZ17I Bsu36I CpoI EagI
Eam1105I EclXI Eco52I Eco81I EcoT22I
FseI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PmeI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SwaI XcmI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 927 CGCG CG^CG
AccIII 1888 TCCGGA T^CCGGA
AflII 3971 CTTAAG C^TTAAG
AgeI 459 ACCGGT A^CCGGT
Alw44I 4197 GTGCAC G^TGCAC
ApaLI 4197 GTGCAC G^TGCAC
AspHI 4197 GTGCAC G[AT]GC[AT]^C
AviII 4493 TGCGCA TGC^GCA
AvrII 2211 CCTAGG C^CTAGG
BciVI 2374 GGATAC GTATCC(6/5)
BfrI 3971 CTTAAG C^TTAAG
BglII 4420 AGATCT A^GATCT
BlnI 2211 CCTAGG C^CTAGG
BlpI 657 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 657 GCTCAGC GC^T[ACGT]AGC
BsaBI 2028 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1888 TCCGGA T^CCGGA
BsiHKAI 4197 GTGCAC G[AT]GC[AT]^C
BsmBI 1372 GAGACG CGTCTC(1/5)
BspDI 2492 ATCGAT AT^CGAT
BspEI 1888 TCCGGA T^CCGGA
BspLU11I 4465 ACATGT A^CATGT
BssHII 926 GCGCGC G^CGCGC
BstAPI 904 GCAAGCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2856 GGTGACC G^GT[ACGT]ACC
BstPI 2856 GGTGACC G^GT[ACGT]ACC
BstUI 927 CGCG CG^CG
CelII 657 GCTCAGC GC^T[ACGT]AGC
ClaI 2492 ATCGAT AT^CGAT
DrdI 1681 GACCCCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2856 GGTGACC G^GT[ACGT]ACC
EcoRV 1699 GATATC GAT^ATC
FauI 1061 CCCGC CCCGC(4/6)
FspI 4493 TGCGCA TGC^GCA
HgaI 1374 GACGC GACGC(5/10)
MamI 2028 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1888 TCCGGA T^CCGGA
MvnI 927 CGCG CG^CG
NcoI 1793 CCATGG C^CATGG
NspI 4465 ACATGT [GA]CATG^[CT]
PaeR7I 1986 CTCGAG C^TCGAG
PinAI 459 ACCGGT A^CCGGT
SapI 1960 GAAGAGC GCTCTTC(1/4)
SpeI 801 ACTAGT A^CTAGT
StuI 3756 AGGCCT AGG^CCT
XbaI 3198 TCTAGA T^CTAGA
XhoI 1986 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 933 ACACGT A^C[GA][CT]GT
4465 ACATGT
Aor51HI 1919 AGCGCT AGC^GCT
3163 AGCGCT
AseI 1468 ATTAAT AT^TAAT
3383 ATTAAT
AsnI 1468 ATTAAT AT^TAAT
3383 ATTAAT
BamHI 475 GGATCC G^GATCC
2603 GGATCC
BclI 3694 TGATCA T^GATCA
3915 TGATCA
BsgI 511 CTGCAC GTGCAG(16/14)
2531 GTGCAG
Bsp1407I 468 TGTACA T^GTACA
4715 TGTACA
BspHI 2658 TCATGA T^CATGA
4651 TCATGA
BsrDI 909 CATTGC GCAATG(2/0)
4278 CATTGC
BsrGI 468 TGTACA T^GTACA
4715 TGTACA
BstXI 200 CCACAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1256 CCAGCACTGTGG
DraIII 1627 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
2851 CACAAGGTG
Eco47III 1919 AGCGCT AGC^GCT
3163 AGCGCT
EcoNI 294 CCTGCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2984 CCTACGACAGG
FbaI 3694 TGATCA T^GATCA
3915 TGATCA
HaeII 1919 AGCGCT [GA]GCGC^[CT]
3163 AGCGCT
MslI 201 CACAGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
966 CACGAGTATG
NaeI 3129 GCCGGC GCC^GGC
4218 GCCGGC
NgoMIV 3129 GCCGGC G^CCGGC
4218 GCCGGC
PshBI 1468 ATTAAT AT^TAAT
3383 ATTAAT
PstI 1348 CTGCAG CTGCA^G
4175 CTGCAG
RcaI 2658 TCATGA T^CATGA
4651 TCATGA
SspBI 468 TGTACA T^GTACA
4715 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI Asp700I AspI
AvaI AvaII BalI BanI BanII
BbrPI BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BsaWI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflFI PflMI PleI PmaCI
PmlI PpuMI PvuII RsaI Sau3AI
Sau96I ScaI ScrFI SexAI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XhoII
XmnI