##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspI AvrII BamHI BbeI
BcgI BclI BlnI BsaHI BsaWI
BseAI BsiWI Bsp1407I BspDI BspEI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI EcoT22I FbaI FseI
Hin1I HpaI KasI KpnI MfeI
MluI MroI MunI NarI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI PvuI
RsrII SalI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 1237 GTAGAC GT^[AC][GT]AC
Asp700I 2791 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 2133 CACGTG CAC^GTG
BbsI 227 GTCTTC GAAGAC(2/6)
BciVI 6080 GTATCC GTATCC(6/5)
BglI 4777 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4845 AGATCT A^GATCT
BpuAI 227 GTCTTC GAAGAC(2/6)
BsaAI 2133 CACGTG [CT]AC^GT[GA]
BsaBI 3046 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2252 CGGCCG CG[GA][CT]^CG
BsmI 1875 GCATTC GAATGC(1/-1)
BsrBI 2249 GAGCGG CCGCTC(-3/-3)
BssSI 3483 CACGAG CACGAG(-5/-1)
BstAPI 1703 GCAGCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 481 GGTCACC G^GT[ACGT]ACC
BstPI 481 GGTCACC G^GT[ACGT]ACC
DraIII 2097 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 473 GACTCCGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2252 CGGCCG C^GGCCG
EclXI 2252 CGGCCG C^GGCCG
Eco52I 2252 CGGCCG C^GGCCG
EcoO65I 481 GGTCACC G^GT[ACGT]ACC
HincII 2261 GTTGAC GT[CT]^[GA]AC
HindII 2261 GTTGAC GT[CT]^[GA]AC
MamI 3046 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1003 GCCGGC GCC^GGC
NgoMIV 1003 GCCGGC G^CCGGC
NheI 4726 GCTAGC G^CTAGC
PmaCI 2133 CACGTG CAC^GTG
PmlI 2133 CACGTG CAC^GTG
PshAI 3999 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 296 CAGCTG CAG^CTG
ScaI 1394 AGTACT AGT^ACT
SexAI 1225 ACCAGGT A^CC[AT]GGT
SmaI 611 CCCGGG CCC^GGG
StuI 2609 AGGCCT AGG^CCT
XmaCI 611 CCCGGG C^CCGGG
XmaI 611 CCCGGG C^CCGGG
XmnI 2791 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AclI 1902 AACGTT AA^CGTT
4296 AACGTT
AflII 5038 CTTAAG C^TTAAG
5075 CTTAAG
AhdI 5456 GACTGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5636 GACTGGCTGTC
Aor51HI 2281 AGCGCT AGC^GCT
3174 AGCGCT
ApaI 570 GGGCCC GGGCC^C
4332 GGGCCC
AspEI 5456 GACTGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5636 GACTGGCTGTC
BalI 459 TGGCCA TGG^CCA
4450 TGGCCA
BbvCI 560 CCTCAGC CCTCAGC(-5/-2)
5328 CCTCAGC
BfrI 5038 CTTAAG C^TTAAG
5075 CTTAAG
BspHI 3079 TCATGA T^CATGA
3428 TCATGA
BspLU11I 3235 ACATGT A^CATGT
3283 ACATGT
BsrFI 1003 GCCGGC [GA]^CCGG[CT]
1387 GCCGGT
Cfr10I 1003 GCCGGC [GA]^CCGG[CT]
1387 GCCGGT
Eam1105I 5456 GACTGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5636 GACTGGCTGTC
Eco47III 2281 AGCGCT AGC^GCT
3174 AGCGCT
EcoNI 4527 CCTTAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5283 CCTTCCTGAGG
HgaI 4832 GACGC GACGC(5/10)
565 GCGTC
KspI 477 CCGCGG CCGC^GG
3196 CCGCGG
MluNI 459 TGGCCA TGG^CCA
4450 TGGCCA
MscI 459 TGGCCA TGG^CCA
4450 TGGCCA
NcoI 2865 CCATGG C^CATGG
3908 CCATGG
NdeI 1299 CATATG CA^TATG
5490 CATATG
PflMI 262 CCACGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1114 CCACAGGCTGG
Psp1406I 1902 AACGTT AA^CGTT
4296 AACGTT
RcaI 3079 TCATGA T^CATGA
3428 TCATGA
SacII 477 CCGCGG CCGC^GG
3196 CCGCGG
Van91I 262 CCACGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1114 CCACAGGCTGG
XbaI 3087 TCTAGA T^CTAGA
5675 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII AviII BanI
BanII BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRI EcoRII
EcoRV EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII