################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AatII Acc65I AccIII AcyI AgeI Alw44I ApaLI AscI AseI AsnI Asp718I AspI AvrII BamHI BbeI BcgI BclI BlnI BsaHI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI EcoT22I FbaI FseI Hin1I HpaI KasI KpnI MfeI MluI MroI MunI NarI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI PvuI RsrII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 1237 GTAGAC GT^[AC][GT]AC Asp700I 2791 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbrPI 2133 CACGTG CAC^GTG BbsI 227 GTCTTC GAAGAC(2/6) BciVI 6080 GTATCC GTATCC(6/5) BglI 4777 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 4845 AGATCT A^GATCT BpuAI 227 GTCTTC GAAGAC(2/6) BsaAI 2133 CACGTG [CT]AC^GT[GA] BsaBI 3046 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 2252 CGGCCG CG[GA][CT]^CG BsmI 1875 GCATTC GAATGC(1/-1) BsrBI 2249 GAGCGG CCGCTC(-3/-3) BssSI 3483 CACGAG CACGAG(-5/-1) BstAPI 1703 GCAGCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 481 GGTCACC G^GT[ACGT]ACC BstPI 481 GGTCACC G^GT[ACGT]ACC DraIII 2097 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 473 GACTCCGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2252 CGGCCG C^GGCCG EclXI 2252 CGGCCG C^GGCCG Eco52I 2252 CGGCCG C^GGCCG EcoO65I 481 GGTCACC G^GT[ACGT]ACC HincII 2261 GTTGAC GT[CT]^[GA]AC HindII 2261 GTTGAC GT[CT]^[GA]AC MamI 3046 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 1003 GCCGGC GCC^GGC NgoMIV 1003 GCCGGC G^CCGGC NheI 4726 GCTAGC G^CTAGC PmaCI 2133 CACGTG CAC^GTG PmlI 2133 CACGTG CAC^GTG PshAI 3999 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 296 CAGCTG CAG^CTG ScaI 1394 AGTACT AGT^ACT SexAI 1225 ACCAGGT A^CC[AT]GGT SmaI 611 CCCGGG CCC^GGG StuI 2609 AGGCCT AGG^CCT XmaCI 611 CCCGGG C^CCGGG XmaI 611 CCCGGG C^CCGGG XmnI 2791 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AclI 1902 AACGTT AA^CGTT 4296 AACGTT AflII 5038 CTTAAG C^TTAAG 5075 CTTAAG AhdI 5456 GACTGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5636 GACTGGCTGTC Aor51HI 2281 AGCGCT AGC^GCT 3174 AGCGCT ApaI 570 GGGCCC GGGCC^C 4332 GGGCCC AspEI 5456 GACTGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5636 GACTGGCTGTC BalI 459 TGGCCA TGG^CCA 4450 TGGCCA BbvCI 560 CCTCAGC CCTCAGC(-5/-2) 5328 CCTCAGC BfrI 5038 CTTAAG C^TTAAG 5075 CTTAAG BspHI 3079 TCATGA T^CATGA 3428 TCATGA BspLU11I 3235 ACATGT A^CATGT 3283 ACATGT BsrFI 1003 GCCGGC [GA]^CCGG[CT] 1387 GCCGGT Cfr10I 1003 GCCGGC [GA]^CCGG[CT] 1387 GCCGGT Eam1105I 5456 GACTGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5636 GACTGGCTGTC Eco47III 2281 AGCGCT AGC^GCT 3174 AGCGCT EcoNI 4527 CCTTAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5283 CCTTCCTGAGG HgaI 4832 GACGC GACGC(5/10) 565 GCGTC KspI 477 CCGCGG CCGC^GG 3196 CCGCGG MluNI 459 TGGCCA TGG^CCA 4450 TGGCCA MscI 459 TGGCCA TGG^CCA 4450 TGGCCA NcoI 2865 CCATGG C^CATGG 3908 CCATGG NdeI 1299 CATATG CA^TATG 5490 CATATG PflMI 262 CCACGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1114 CCACAGGCTGG Psp1406I 1902 AACGTT AA^CGTT 4296 AACGTT RcaI 3079 TCATGA T^CATGA 3428 TCATGA SacII 477 CCGCGG CCGC^GG 3196 CCGCGG Van91I 262 CCACGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1114 CCACAGGCTGG XbaI 3087 TCTAGA T^CTAGA 5675 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII AviII BanI BanII BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoO109I EcoRI EcoRII EcoRV EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII