##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Aor51HI AscI
AseI AsnI Asp718I AspEI AviII
BbeI BbrPI BcgI BfrI BsaBI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BsrBI BsrFI BsrGI
BssHII BstBI BstEII BstPI BstUI
CfoI Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI FseI FspI HaeII
HhaI HinP1I KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NgoMIV NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI Sse8387I SspBI SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AcyI 961 GGCGTC G[GA]^CG[CT]C
Alw44I 4456 GTGCAC G^TGCAC
ApaLI 4456 GTGCAC G^TGCAC
Asp700I 592 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 4131 TGGCCA TGG^CCA
BamHI 2148 GGATCC G^GATCC
BbvCI 3644 GCTGAGG CCTCAGC(-5/-2)
BclI 357 TGATCA T^GATCA
BglII 2178 AGATCT A^GATCT
BsaHI 961 GGCGTC G[GA]^CG[CT]C
BsaWI 288 TCCGGT [AT]^CCGG[AT]
BssSI 1245 CTCGTG CACGAG(-5/-1)
Bst1107I 4986 GTATAC GTA^TAC
BstAPI 401 GCAGACAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 4986 GTATAC GTA^TAC
DsaI 3582 CCATGG C^C[GA][CT]GG
EaeI 4131 TGGCCA [CT]^GGCC[GA]
EcoRV 4511 GATATC GAT^ATC
EcoT22I 4597 ATGCAT ATGCA^T
FauI 294 CCCGC CCCGC(4/6)
FbaI 357 TGATCA T^GATCA
HgaI 962 GCGTC GACGC(5/10)
Hin1I 961 GGCGTC G[GA]^CG[CT]C
MluNI 4131 TGGCCA TGG^CCA
MscI 4131 TGGCCA TGG^CCA
NcoI 3582 CCATGG C^CATGG
NheI 4951 GCTAGC G^CTAGC
NsiI 4597 ATGCAT ATGCA^T
PshAI 3733 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1242 GAGCTC GAGCT^C
SexAI 183 ACCTGGT A^CC[AT]GGT
SnaBI 438 TACGTA TAC^GTA
SpeI 2046 ACTAGT A^CTAGT
SphI 4595 GCATGC GCATG^C
TaqI 4358 TCGA T^CGA
TthHB8I 4358 TCGA T^CGA
XcmI 2973 CCACCCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 592 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
ApaI 1145 GGGCCC GGGCC^C
2761 GGGCCC
AspI 3823 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3990 GACCTCGTC
AvaI 3175 CCCGGG C^[CT]CG[GA]G
4715 CCCGGG
BbsI 3325 GAAGAC GAAGAC(2/6)
3871 GTCTTC
BpuAI 3325 GAAGAC GAAGAC(2/6)
3871 GTCTTC
BsaAI 438 TACGTA [CT]AC^GT[GA]
1738 CACGTA
BsmBI 805 GAGACG CGTCTC(1/5)
1859 GAGACG
BsoBI 3175 CCCGGG C^[CT]CG[GA]G
4715 CCCGGG
BspHI 954 TCATGA T^CATGA
2402 TCATGA
DraI 990 TTTAAA TTT^AAA
1480 TTTAAA
DraIII 1524 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
3886 CACATTGTG
HindIII 1881 AAGCTT A^AGCTT
2960 AAGCTT
HpaI 2042 GTTAAC GTT^AAC
2063 GTTAAC
PflFI 3823 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3990 GACCTCGTC
PstI 2724 CTGCAG CTGCA^G
4705 CTGCAG
RcaI 954 TCATGA T^CATGA
2402 TCATGA
SmaI 3175 CCCGGG CCC^GGG
4715 CCCGGG
StuI 998 AGGCCT AGG^CCT
1497 AGGCCT
Tth111I 3823 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3990 GACCTCGTC
XmaCI 3175 CCCGGG C^CCGGG
4715 CCCGGG
XmaI 3175 CCCGGG C^CCGGG
4715 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaII AvrII BanI BanII
BbvI BciVI BcnI BfaI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspLU11I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII Cfr13I DdeI DpnII
DraII EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XhoII