##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI Alw44I ApaLI AscI AseI
AsnI BbeI BcgI BciVI BfrI
BsaHI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspHI BspLU11I BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DrdI EagI EclXI Eco52I EcoRI
EcoRV EcoT22I FseI Hin1I HpaI
KasI KspI MluI MroI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 793 GGTACC G^GTACC
AccI 4389 GTCTAC GT^[AC][GT]AC
Aor51HI 2881 AGCGCT AGC^GCT
Asp700I 702 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 793 GGTACC G^GTACC
BamHI 2306 GGATCC G^GATCC
BbrPI 1942 CACGTG CAC^GTG
BglII 1838 AGATCT A^GATCT
BsaBI 78 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrBI 4702 CCGCTC CCGCTC(-3/-3)
BstEII 945 GGTCACC G^GT[ACGT]ACC
BstPI 945 GGTCACC G^GT[ACGT]ACC
DraI 1293 TTTAAA TTT^AAA
Eco47III 2881 AGCGCT AGC^GCT
EcoO65I 945 GGTCACC G^GT[ACGT]ACC
HindIII 1103 AAGCTT A^AGCTT
KpnI 793 GGTACC GGTAC^C
MamI 78 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2369 CAATTG C^AATTG
MunI 2369 CAATTG C^AATTG
PmaCI 1942 CACGTG CAC^GTG
PmlI 1942 CACGTG CAC^GTG
SbfI 3348 CCTGCAGG CCTGCA^GG
ScaI 2592 AGTACT AGT^ACT
SexAI 2181 ACCAGGT A^CC[AT]GGT
SphI 4402 GCATGC GCATG^C
Sse8387I 3348 CCTGCAGG CCTGCA^GG
XbaI 1275 TCTAGA T^CTAGA
XmnI 702 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 1941 ACACGT A^C[GA][CT]GT
4936 ACACGT
AhdI 991 GACGAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1330 GACCAACTGTC
AspEI 991 GACGAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1330 GACCAACTGTC
AspI 874 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3997 GACCCTGTC
BclI 1646 TGATCA T^GATCA
2342 TGATCA
BsmBI 4546 CGTCTC CGTCTC(1/5)
3875 GAGACG
BssSI 301 CACGAG CACGAG(-5/-1)
2576 CACGAG
BstXI 2330 CCAGCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3597 CCATGTGTATGG
DraIII 301 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2892 CACTATGTG
Eam1105I 991 GACGAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1330 GACCAACTGTC
FbaI 1646 TGATCA T^GATCA
2342 TGATCA
NaeI 1846 GCCGGC GCC^GGC
4959 GCCGGC
NgoMIV 1846 GCCGGC G^CCGGC
4959 GCCGGC
PflFI 874 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3997 GACCCTGTC
PshAI 2692 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3504 GACTGTGGTC
SacI 235 GAGCTC GAGCT^C
316 GAGCTC
SapI 5018 GAAGAGC GCTCTTC(1/4)
4704 GCTCTTC
Tth111I 874 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3997 GACCCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII AviII AvrII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrDI BsrFI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI